Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases
Rapid detection of infectious diseases is critical for global public health prevention and control. However, the use of traditional molecular diagnostic methods, including PCR, has been limited because of their cumbersome procedures, complex equipment requirements, operation at different temperature...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1601644/full |
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| author | Qingshang Bi Mengru Liu Mengru Liu Li Yan Jun Cheng Qingyang Sun Yuzhu Dai Lingli Zou |
| author_facet | Qingshang Bi Mengru Liu Mengru Liu Li Yan Jun Cheng Qingyang Sun Yuzhu Dai Lingli Zou |
| author_sort | Qingshang Bi |
| collection | DOAJ |
| description | Rapid detection of infectious diseases is critical for global public health prevention and control. However, the use of traditional molecular diagnostic methods, including PCR, has been limited because of their cumbersome procedures, complex equipment requirements, operation at different temperatures, and the level of expertise required for operation. Isothermal amplification technology (IAT) provides a rapid, sensitive, specific, simple and less costly method for diagnosing infectious diseases, which has led to revolutionary breakthroughs in molecular diagnostics. This paper summarizes recent progress in IAT technology, which focuses on the principles and applications of core technologies such as NASBA, LAMP, RPA, and RAA. In addition, the combination of IATs with the CRISPR/Cas system, which further revolutionizes nucleic acid detection technology, is explored in this review. |
| format | Article |
| id | doaj-art-b1d0c7d440664d82ade07678fe82eefe |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-b1d0c7d440664d82ade07678fe82eefe2025-08-20T03:29:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.16016441601644Progress in the application of isothermal amplification technology in the diagnosis of infectious diseasesQingshang Bi0Mengru Liu1Mengru Liu2Li Yan3Jun Cheng4Qingyang Sun5Yuzhu Dai6Lingli Zou7Department of Clinical Laboratory, Third Sanatorium, Air Force Healthcare Center for Special Services, Hangzhou, ChinaSchool of Laboratory Medicine, Bengbu Medical University, Bengbu, ChinaDepartment of Clinical Laboratory, The 903rd Hospital of The People’s Liberation Army, Zhejiang, ChinaDepartment of Health Economic, The 903rd Hospital of The People’s Liberation Army, Zhejiang, ChinaDepartment of Clinical Laboratory, The 903rd Hospital of The People’s Liberation Army, Zhejiang, ChinaDepartment of Clinical Laboratory, The 903rd Hospital of The People’s Liberation Army, Zhejiang, ChinaDepartment of Clinical Laboratory, The 903rd Hospital of The People’s Liberation Army, Zhejiang, ChinaDepartment of Clinical Laboratory, Third Sanatorium, Air Force Healthcare Center for Special Services, Hangzhou, ChinaRapid detection of infectious diseases is critical for global public health prevention and control. However, the use of traditional molecular diagnostic methods, including PCR, has been limited because of their cumbersome procedures, complex equipment requirements, operation at different temperatures, and the level of expertise required for operation. Isothermal amplification technology (IAT) provides a rapid, sensitive, specific, simple and less costly method for diagnosing infectious diseases, which has led to revolutionary breakthroughs in molecular diagnostics. This paper summarizes recent progress in IAT technology, which focuses on the principles and applications of core technologies such as NASBA, LAMP, RPA, and RAA. In addition, the combination of IATs with the CRISPR/Cas system, which further revolutionizes nucleic acid detection technology, is explored in this review.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1601644/fullisothermal amplification technologyinfectious diseasesCRISPR/Cas systemmolecular diagnosticsnucleic acid amplification |
| spellingShingle | Qingshang Bi Mengru Liu Mengru Liu Li Yan Jun Cheng Qingyang Sun Yuzhu Dai Lingli Zou Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases Frontiers in Microbiology isothermal amplification technology infectious diseases CRISPR/Cas system molecular diagnostics nucleic acid amplification |
| title | Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases |
| title_full | Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases |
| title_fullStr | Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases |
| title_full_unstemmed | Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases |
| title_short | Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases |
| title_sort | progress in the application of isothermal amplification technology in the diagnosis of infectious diseases |
| topic | isothermal amplification technology infectious diseases CRISPR/Cas system molecular diagnostics nucleic acid amplification |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1601644/full |
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