Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCR

ABSTRACT Freshwater mussels are traditionally monitored by visual observations which is time‐consuming and can be difficult depending on water conditions. Environmental DNA (eDNA) is an attractive alternative since it can detect a species in the water without visual observations. Furthermore, since...

Full description

Saved in:
Bibliographic Details
Main Authors: Per Sundberg, Alizz Axberg, Nauras Daragmeh, Niklas Wengström, Marina Panova
Format: Article
Language:English
Published: Wiley 2024-11-01
Series:Environmental DNA
Subjects:
Online Access:https://doi.org/10.1002/edn3.70046
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832582970339229696
author Per Sundberg
Alizz Axberg
Nauras Daragmeh
Niklas Wengström
Marina Panova
author_facet Per Sundberg
Alizz Axberg
Nauras Daragmeh
Niklas Wengström
Marina Panova
author_sort Per Sundberg
collection DOAJ
description ABSTRACT Freshwater mussels are traditionally monitored by visual observations which is time‐consuming and can be difficult depending on water conditions. Environmental DNA (eDNA) is an attractive alternative since it can detect a species in the water without visual observations. Furthermore, since the DNA can potentially travel downstream in the river systems, presence of a species can be detected even away from the population of mussels. In this paper, we evaluate and describe how dPCR (digital PCR) technique can be used to efficiently monitor four freshwater mussel species: the freshwater pearl mussel Margaritifera margaritifera from Margaritiferidae family and three species from Unionidae family: the depressed river mussel Pseudanodonta complanata, the painter's mussel Unio pictorum, and the thick shelled river mussel Unio crassus, which are all regarded endangered in many regions worldwide. dPCR assays for the four mussel species were developed and tested in silico before conducting field surveys. The verification step in the field was carried out in two Swedish rivers with confirmed populations of the freshwater pearl mussel (M. margaritifera). Furthermore, two other rivers with unknown presence of the endangered freshwater mussels were surveyed for occurrence of the four mussel species, using the capacity to multiplex several species simultaneously in a dPCR reaction. We furthermore showed that the target DNA concentrations probably depend on the season and water level, which may largely affect the detection probability and interpretation of the results in terms of population size.
format Article
id doaj-art-b1cfd1ef880c453eaa297db10ce47395
institution Kabale University
issn 2637-4943
language English
publishDate 2024-11-01
publisher Wiley
record_format Article
series Environmental DNA
spelling doaj-art-b1cfd1ef880c453eaa297db10ce473952025-01-29T05:11:50ZengWileyEnvironmental DNA2637-49432024-11-0166n/an/a10.1002/edn3.70046Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCRPer Sundberg0Alizz Axberg1Nauras Daragmeh2Niklas Wengström3Marina Panova4Department of Marine Sciences University of Gothenburg Goteborg SwedenDepartment of Marine Sciences University of Gothenburg Goteborg SwedenDepartment of Marine Sciences University of Gothenburg Goteborg SwedenSwedish Anglers Association Gothenburg SwedenDepartment of Marine Sciences University of Gothenburg Goteborg SwedenABSTRACT Freshwater mussels are traditionally monitored by visual observations which is time‐consuming and can be difficult depending on water conditions. Environmental DNA (eDNA) is an attractive alternative since it can detect a species in the water without visual observations. Furthermore, since the DNA can potentially travel downstream in the river systems, presence of a species can be detected even away from the population of mussels. In this paper, we evaluate and describe how dPCR (digital PCR) technique can be used to efficiently monitor four freshwater mussel species: the freshwater pearl mussel Margaritifera margaritifera from Margaritiferidae family and three species from Unionidae family: the depressed river mussel Pseudanodonta complanata, the painter's mussel Unio pictorum, and the thick shelled river mussel Unio crassus, which are all regarded endangered in many regions worldwide. dPCR assays for the four mussel species were developed and tested in silico before conducting field surveys. The verification step in the field was carried out in two Swedish rivers with confirmed populations of the freshwater pearl mussel (M. margaritifera). Furthermore, two other rivers with unknown presence of the endangered freshwater mussels were surveyed for occurrence of the four mussel species, using the capacity to multiplex several species simultaneously in a dPCR reaction. We furthermore showed that the target DNA concentrations probably depend on the season and water level, which may largely affect the detection probability and interpretation of the results in terms of population size.https://doi.org/10.1002/edn3.70046digital PCReDNAendangered speciesfreshwater musselmonitoring
spellingShingle Per Sundberg
Alizz Axberg
Nauras Daragmeh
Niklas Wengström
Marina Panova
Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCR
Environmental DNA
digital PCR
eDNA
endangered species
freshwater mussel
monitoring
title Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCR
title_full Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCR
title_fullStr Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCR
title_full_unstemmed Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCR
title_short Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCR
title_sort monitoring of endangered freshwater mussels in sweden using digital pcr
topic digital PCR
eDNA
endangered species
freshwater mussel
monitoring
url https://doi.org/10.1002/edn3.70046
work_keys_str_mv AT persundberg monitoringofendangeredfreshwatermusselsinswedenusingdigitalpcr
AT alizzaxberg monitoringofendangeredfreshwatermusselsinswedenusingdigitalpcr
AT naurasdaragmeh monitoringofendangeredfreshwatermusselsinswedenusingdigitalpcr
AT niklaswengstrom monitoringofendangeredfreshwatermusselsinswedenusingdigitalpcr
AT marinapanova monitoringofendangeredfreshwatermusselsinswedenusingdigitalpcr