Chromosome-level genome assembly, annotation, and population genomic resource of argali (Ovis ammon)
Abstract Argali stands as the largest species among wild sheep in Central and East Asia, with a concerning rate of decline estimated at 30%. The intraspecific taxonomy of argali remains contentious due to limited genomic data and unclear geographic separation. In this study, we constructed a chromos...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04400-6 |
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| author | Mu-Yang Wang Bao-Lin Zhang Qi-Qi Liang Xin-Ming Lian Ke Zhang Qi-En Yang Wei-Kang Yang |
| author_facet | Mu-Yang Wang Bao-Lin Zhang Qi-Qi Liang Xin-Ming Lian Ke Zhang Qi-En Yang Wei-Kang Yang |
| author_sort | Mu-Yang Wang |
| collection | DOAJ |
| description | Abstract Argali stands as the largest species among wild sheep in Central and East Asia, with a concerning rate of decline estimated at 30%. The intraspecific taxonomy of argali remains contentious due to limited genomic data and unclear geographic separation. In this study, we constructed a chromosome-level genome assembly and annotation for the Tibetan argali (O. a. hodgsoni), together with population genomic resequencing of 32 individuals representing four subspecies. The contig-level genome was 2.64 Gb in size, with a contig N50 length of 71.69 Mb and an estimated genomic completeness of 96.01%. Using Hi-C sequencing data scaffolding, 99.90% of initially assembled sequences were mapped and oriented onto 28 pseudo-chromosomes except the Y chromosome. Annotation uncovered 21,564 protein-coding genes and 46.38% repeat sequences. The average coverage of the population resequencing data was 23.74 with mean mapping ratio up to of 97.19%. The high-quality genome assembly and annotation of the Tibetan argali, coupled with the high-depth population genomic data, will serve as a valuable genetic resource for studies on the taxonomy and conservation of argali. |
| format | Article |
| id | doaj-art-b1c0584ab4704ebdb614c4748772aff7 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
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| spelling | doaj-art-b1c0584ab4704ebdb614c4748772aff72025-01-12T12:07:35ZengNature PortfolioScientific Data2052-44632025-01-0112111010.1038/s41597-025-04400-6Chromosome-level genome assembly, annotation, and population genomic resource of argali (Ovis ammon)Mu-Yang Wang0Bao-Lin Zhang1Qi-Qi Liang2Xin-Ming Lian3Ke Zhang4Qi-En Yang5Wei-Kang Yang6Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of SciencesKey Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, Chinese Academy of SciencesBeijing Bio Huaxing Gene Technology Co., LTDsKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of SciencesKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of SciencesKey Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of SciencesKey Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of SciencesAbstract Argali stands as the largest species among wild sheep in Central and East Asia, with a concerning rate of decline estimated at 30%. The intraspecific taxonomy of argali remains contentious due to limited genomic data and unclear geographic separation. In this study, we constructed a chromosome-level genome assembly and annotation for the Tibetan argali (O. a. hodgsoni), together with population genomic resequencing of 32 individuals representing four subspecies. The contig-level genome was 2.64 Gb in size, with a contig N50 length of 71.69 Mb and an estimated genomic completeness of 96.01%. Using Hi-C sequencing data scaffolding, 99.90% of initially assembled sequences were mapped and oriented onto 28 pseudo-chromosomes except the Y chromosome. Annotation uncovered 21,564 protein-coding genes and 46.38% repeat sequences. The average coverage of the population resequencing data was 23.74 with mean mapping ratio up to of 97.19%. The high-quality genome assembly and annotation of the Tibetan argali, coupled with the high-depth population genomic data, will serve as a valuable genetic resource for studies on the taxonomy and conservation of argali.https://doi.org/10.1038/s41597-025-04400-6 |
| spellingShingle | Mu-Yang Wang Bao-Lin Zhang Qi-Qi Liang Xin-Ming Lian Ke Zhang Qi-En Yang Wei-Kang Yang Chromosome-level genome assembly, annotation, and population genomic resource of argali (Ovis ammon) Scientific Data |
| title | Chromosome-level genome assembly, annotation, and population genomic resource of argali (Ovis ammon) |
| title_full | Chromosome-level genome assembly, annotation, and population genomic resource of argali (Ovis ammon) |
| title_fullStr | Chromosome-level genome assembly, annotation, and population genomic resource of argali (Ovis ammon) |
| title_full_unstemmed | Chromosome-level genome assembly, annotation, and population genomic resource of argali (Ovis ammon) |
| title_short | Chromosome-level genome assembly, annotation, and population genomic resource of argali (Ovis ammon) |
| title_sort | chromosome level genome assembly annotation and population genomic resource of argali ovis ammon |
| url | https://doi.org/10.1038/s41597-025-04400-6 |
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