Pretreatment of Vine Shoot Biomass by Choline Chloride-Based Deep Eutectic Solvents to Promote Biomass Fractionation and Enhance Sugar Production
Vine shoots hold promise as a biomass source for fermentable sugars with efficient fractionation and conversion processes. The study explores vine shoots as a biomass source for fermentable sugars through pretreatment with two deep eutectic solvents mixtures: choline chloride:lactic acid 1:5 (ChCl:L...
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MDPI AG
2024-09-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/11/9/935 |
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| author | Raquel Cañadas Aleta Duque Alberto Bahíllo Raquel Iglesias Paloma Manzanares |
| author_facet | Raquel Cañadas Aleta Duque Alberto Bahíllo Raquel Iglesias Paloma Manzanares |
| author_sort | Raquel Cañadas |
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| description | Vine shoots hold promise as a biomass source for fermentable sugars with efficient fractionation and conversion processes. The study explores vine shoots as a biomass source for fermentable sugars through pretreatment with two deep eutectic solvents mixtures: choline chloride:lactic acid 1:5 (ChCl:LA) and choline chloride:ethylene glycol 1:2 (ChCl:EG). Pretreatment conditions, such as temperature/time, solid/liquid ratio, and biomass particle size, were studied. Chemical composition, recovery yields, delignification extent, and carbohydrate conversion were evaluated, including the influence of washing solvents. Temperature and particle size notably affected hemicellulose and lignin dissolution, especially with ChCl:LA. Pretreatment yielded enriched cellulose substrates, with high carbohydrate conversion rates up to 75.2% for cellulose and 99.9% for xylan with ChCl:LA, and 54.6% for cellulose and 60.2% for xylan with ChCl:EG. A 50% acetone/water mixture increased the delignification ratios to 31.5%. The results underscore the potential of this pretreatment for vine shoot fractionation, particularly at 30% solid load, while acknowledging the need for further process enhancement. |
| format | Article |
| id | doaj-art-b1be01c0fa624d7295dfbf42fd955705 |
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| issn | 2306-5354 |
| language | English |
| publishDate | 2024-09-01 |
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| series | Bioengineering |
| spelling | doaj-art-b1be01c0fa624d7295dfbf42fd9557052025-08-20T01:56:10ZengMDPI AGBioengineering2306-53542024-09-0111993510.3390/bioengineering11090935Pretreatment of Vine Shoot Biomass by Choline Chloride-Based Deep Eutectic Solvents to Promote Biomass Fractionation and Enhance Sugar ProductionRaquel Cañadas0Aleta Duque1Alberto Bahíllo2Raquel Iglesias3Paloma Manzanares4Advanced Biofuels and Bioproducts Unit, Department of Energy, Research Centre for Energy, Environment and Technology (CIEMAT), 28040 Madrid, SpainAdvanced Biofuels and Bioproducts Unit, Department of Energy, Research Centre for Energy, Environment and Technology (CIEMAT), 28040 Madrid, SpainSustainable Thermochemical Valorization Unit, Department of Energy, Research Centre for Energy, Environment and Technology (CIEMAT), 28040 Madrid, SpainAdvanced Biofuels and Bioproducts Unit, Department of Energy, Research Centre for Energy, Environment and Technology (CIEMAT), 28040 Madrid, SpainAdvanced Biofuels and Bioproducts Unit, Department of Energy, Research Centre for Energy, Environment and Technology (CIEMAT), 28040 Madrid, SpainVine shoots hold promise as a biomass source for fermentable sugars with efficient fractionation and conversion processes. The study explores vine shoots as a biomass source for fermentable sugars through pretreatment with two deep eutectic solvents mixtures: choline chloride:lactic acid 1:5 (ChCl:LA) and choline chloride:ethylene glycol 1:2 (ChCl:EG). Pretreatment conditions, such as temperature/time, solid/liquid ratio, and biomass particle size, were studied. Chemical composition, recovery yields, delignification extent, and carbohydrate conversion were evaluated, including the influence of washing solvents. Temperature and particle size notably affected hemicellulose and lignin dissolution, especially with ChCl:LA. Pretreatment yielded enriched cellulose substrates, with high carbohydrate conversion rates up to 75.2% for cellulose and 99.9% for xylan with ChCl:LA, and 54.6% for cellulose and 60.2% for xylan with ChCl:EG. A 50% acetone/water mixture increased the delignification ratios to 31.5%. The results underscore the potential of this pretreatment for vine shoot fractionation, particularly at 30% solid load, while acknowledging the need for further process enhancement.https://www.mdpi.com/2306-5354/11/9/935agricultural residuebiomass fractionationgreen solventenzymatic hydrolysislignin |
| spellingShingle | Raquel Cañadas Aleta Duque Alberto Bahíllo Raquel Iglesias Paloma Manzanares Pretreatment of Vine Shoot Biomass by Choline Chloride-Based Deep Eutectic Solvents to Promote Biomass Fractionation and Enhance Sugar Production Bioengineering agricultural residue biomass fractionation green solvent enzymatic hydrolysis lignin |
| title | Pretreatment of Vine Shoot Biomass by Choline Chloride-Based Deep Eutectic Solvents to Promote Biomass Fractionation and Enhance Sugar Production |
| title_full | Pretreatment of Vine Shoot Biomass by Choline Chloride-Based Deep Eutectic Solvents to Promote Biomass Fractionation and Enhance Sugar Production |
| title_fullStr | Pretreatment of Vine Shoot Biomass by Choline Chloride-Based Deep Eutectic Solvents to Promote Biomass Fractionation and Enhance Sugar Production |
| title_full_unstemmed | Pretreatment of Vine Shoot Biomass by Choline Chloride-Based Deep Eutectic Solvents to Promote Biomass Fractionation and Enhance Sugar Production |
| title_short | Pretreatment of Vine Shoot Biomass by Choline Chloride-Based Deep Eutectic Solvents to Promote Biomass Fractionation and Enhance Sugar Production |
| title_sort | pretreatment of vine shoot biomass by choline chloride based deep eutectic solvents to promote biomass fractionation and enhance sugar production |
| topic | agricultural residue biomass fractionation green solvent enzymatic hydrolysis lignin |
| url | https://www.mdpi.com/2306-5354/11/9/935 |
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