Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication
Our investigations focused on spindle dynamics/displacement in frozen-thawed human oocytes. In each oocyte, prior to freezing and after thawing and culturing, the presence/location of the spindle was determined with the Polscope technique. A total of 259 oocytes have been thawed with a survival rate...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/785421 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563861861957632 |
|---|---|
| author | János Konc Katalin Kanyo Rita Kriston József Zeke Sándor Cseh |
| author_facet | János Konc Katalin Kanyo Rita Kriston József Zeke Sándor Cseh |
| author_sort | János Konc |
| collection | DOAJ |
| description | Our investigations focused on spindle dynamics/displacement in frozen-thawed human oocytes. In each oocyte, prior to freezing and after thawing and culturing, the presence/location of the spindle was determined with the Polscope technique. A total of 259 oocytes have been thawed with a survival rate of 81.1%. From the 210 survived oocytes, 165 were fertilized (78.6%) and 89.1% of them cleaved. A total of 143 embryos were transferred into 63 patients resulting in 11 clinical pregnancies (17.5%), 7 of which resulted in live birth of 8 babies (1 twin pregnancy). We were able to detect the spindle in 221 of 259 oocytes (85.3%). After thawing and culturing the oocytes, we were able to visualize the spindle in 177 of 210 oocytes (84.3%). In 83 of these 177 oocytes, the spindle was observed to be in the same location as it was before cryopreservation (46.9%). However, in 94 of these 177 oocytes (53.1%), the spindle reformed in a different position/location relative to the polar body. Our results show that after thawing and culture in half of the spindle-positive oocytes the spindle was detected in a new location, indicating that the spindle and the polar body move relative to each other. |
| format | Article |
| id | doaj-art-b1bab09c77324416827d0358f6eb7fb3 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-b1bab09c77324416827d0358f6eb7fb32025-02-03T01:12:21ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/785421785421Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short CommunicationJános Konc0Katalin Kanyo1Rita Kriston2József Zeke3Sándor Cseh4Infertility and IVF Center of Buda, Szent Janos Hospital, Budapest 1125, HungaryInfertility and IVF Center of Buda, Szent Janos Hospital, Budapest 1125, HungaryInfertility and IVF Center of Buda, Szent Janos Hospital, Budapest 1125, HungaryInfertility and IVF Center of Buda, Szent Janos Hospital, Budapest 1125, HungaryFaculty of Veterinary Science, Szent István University, István u. 2, Budapest 1078, HungaryOur investigations focused on spindle dynamics/displacement in frozen-thawed human oocytes. In each oocyte, prior to freezing and after thawing and culturing, the presence/location of the spindle was determined with the Polscope technique. A total of 259 oocytes have been thawed with a survival rate of 81.1%. From the 210 survived oocytes, 165 were fertilized (78.6%) and 89.1% of them cleaved. A total of 143 embryos were transferred into 63 patients resulting in 11 clinical pregnancies (17.5%), 7 of which resulted in live birth of 8 babies (1 twin pregnancy). We were able to detect the spindle in 221 of 259 oocytes (85.3%). After thawing and culturing the oocytes, we were able to visualize the spindle in 177 of 210 oocytes (84.3%). In 83 of these 177 oocytes, the spindle was observed to be in the same location as it was before cryopreservation (46.9%). However, in 94 of these 177 oocytes (53.1%), the spindle reformed in a different position/location relative to the polar body. Our results show that after thawing and culture in half of the spindle-positive oocytes the spindle was detected in a new location, indicating that the spindle and the polar body move relative to each other.http://dx.doi.org/10.1100/2012/785421 |
| spellingShingle | János Konc Katalin Kanyo Rita Kriston József Zeke Sándor Cseh Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication The Scientific World Journal |
| title | Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication |
| title_full | Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication |
| title_fullStr | Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication |
| title_full_unstemmed | Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication |
| title_short | Freezing of Oocytes and Its Effect on the Displacement of the Meiotic Spindle: Short Communication |
| title_sort | freezing of oocytes and its effect on the displacement of the meiotic spindle short communication |
| url | http://dx.doi.org/10.1100/2012/785421 |
| work_keys_str_mv | AT janoskonc freezingofoocytesanditseffectonthedisplacementofthemeioticspindleshortcommunication AT katalinkanyo freezingofoocytesanditseffectonthedisplacementofthemeioticspindleshortcommunication AT ritakriston freezingofoocytesanditseffectonthedisplacementofthemeioticspindleshortcommunication AT jozsefzeke freezingofoocytesanditseffectonthedisplacementofthemeioticspindleshortcommunication AT sandorcseh freezingofoocytesanditseffectonthedisplacementofthemeioticspindleshortcommunication |