Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes Progression

Type 2 diabetes is considered as a chronic inflammatory disease in which the dense microvasculature reorganizes with disease progression and is highly correlated with β cell mass and islet function. In this study, we constructed rat models of type 2 diabetes and used ultrasound localization microsco...

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Main Authors: Tao Zhang, Jipeng Yan, Xinhuan Zhou, Bihan Wu, Chao Zhang, Mengxing Tang, Pintong Huang
Format: Article
Language:English
Published: American Association for the Advancement of Science (AAAS) 2025-01-01
Series:Cyborg and Bionic Systems
Online Access:https://spj.science.org/doi/10.34133/cbsystems.0117
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author Tao Zhang
Jipeng Yan
Xinhuan Zhou
Bihan Wu
Chao Zhang
Mengxing Tang
Pintong Huang
author_facet Tao Zhang
Jipeng Yan
Xinhuan Zhou
Bihan Wu
Chao Zhang
Mengxing Tang
Pintong Huang
author_sort Tao Zhang
collection DOAJ
description Type 2 diabetes is considered as a chronic inflammatory disease in which the dense microvasculature reorganizes with disease progression and is highly correlated with β cell mass and islet function. In this study, we constructed rat models of type 2 diabetes and used ultrasound localization microscopy (ULM) imaging to noninvasively map the pancreatic microvasculature at microscopy resolution in vivo to reflect β cell loss and islet function deterioration, and evaluate the efficacy after anti-cytokine immunotherapy. It was unveiled that ULM morphological and hemodynamic parameters have a strong link with β cell loss and deterioration of pancreatic islet function. This correlation aligns with the observed pathological alterations in the microvessels of islet and demonstrated that ULM can effectively mirror the functionality of β cells during rapid fluctuations in blood glucose levels by observing changes in mean velocity. Furthermore, it was revealed that treatment with anti-cytokine immunotherapy enhances the function and health of β cells by restoring the microvascular environment. Remarkable improvements in vessel morphology (measured by fractal dimension) and hemodynamics (indicated by mean velocity and vessel density) were noted following the anti-cytokine immunotherapy, signifying a significant enhancement at the treatment’s conclusion (P < 0.05). These observations suggested that ULM technology holds promise as a visible and efficient tool for monitoring the effectiveness of anti-cytokine immunotherapy in managing type 2 diabetes. Pancreatic microvessel-based ULM may serve as a novel noninvasive method to assess β cells, providing a valuable clinical tool for tracking the progression of type 2 diabetes.
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spelling doaj-art-b1b89ff62a3b4b80a687204e04b4e9a82025-08-20T03:12:20ZengAmerican Association for the Advancement of Science (AAAS)Cyborg and Bionic Systems2692-76322025-01-01610.34133/cbsystems.0117Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes ProgressionTao Zhang0Jipeng Yan1Xinhuan Zhou2Bihan Wu3Chao Zhang4Mengxing Tang5Pintong Huang6Department of Ultrasound in Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China.Ultrasound Lab for Imaging and Sensing, Department of Bioengineering, Imperial College London, London, UK.Ultrasound Lab for Imaging and Sensing, Department of Bioengineering, Imperial College London, London, UK.Department of Ultrasound in Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China.Department of Ultrasound in Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China.Ultrasound Lab for Imaging and Sensing, Department of Bioengineering, Imperial College London, London, UK.Department of Ultrasound in Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China.Type 2 diabetes is considered as a chronic inflammatory disease in which the dense microvasculature reorganizes with disease progression and is highly correlated with β cell mass and islet function. In this study, we constructed rat models of type 2 diabetes and used ultrasound localization microscopy (ULM) imaging to noninvasively map the pancreatic microvasculature at microscopy resolution in vivo to reflect β cell loss and islet function deterioration, and evaluate the efficacy after anti-cytokine immunotherapy. It was unveiled that ULM morphological and hemodynamic parameters have a strong link with β cell loss and deterioration of pancreatic islet function. This correlation aligns with the observed pathological alterations in the microvessels of islet and demonstrated that ULM can effectively mirror the functionality of β cells during rapid fluctuations in blood glucose levels by observing changes in mean velocity. Furthermore, it was revealed that treatment with anti-cytokine immunotherapy enhances the function and health of β cells by restoring the microvascular environment. Remarkable improvements in vessel morphology (measured by fractal dimension) and hemodynamics (indicated by mean velocity and vessel density) were noted following the anti-cytokine immunotherapy, signifying a significant enhancement at the treatment’s conclusion (P < 0.05). These observations suggested that ULM technology holds promise as a visible and efficient tool for monitoring the effectiveness of anti-cytokine immunotherapy in managing type 2 diabetes. Pancreatic microvessel-based ULM may serve as a novel noninvasive method to assess β cells, providing a valuable clinical tool for tracking the progression of type 2 diabetes.https://spj.science.org/doi/10.34133/cbsystems.0117
spellingShingle Tao Zhang
Jipeng Yan
Xinhuan Zhou
Bihan Wu
Chao Zhang
Mengxing Tang
Pintong Huang
Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes Progression
Cyborg and Bionic Systems
title Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes Progression
title_full Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes Progression
title_fullStr Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes Progression
title_full_unstemmed Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes Progression
title_short Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes Progression
title_sort application of ultrasound localization microscopy in evaluating the type 2 diabetes progression
url https://spj.science.org/doi/10.34133/cbsystems.0117
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