A lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans

Abstract Lectins are produced in almost all life forms, can interact with targets (glycans) in a cross-kingdom manner and have served as valuable tools for studying glycobiology. Previously, a bacterial lectin, named Streptomyces hemagglutinin (SHA), was found to agglutinate human type B erythrocyte...

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Main Authors: Janine C. Quijano, Honoka Natsuyama, Alonso Tapia, Karine Bagramyan, Jose A. Ortiz, Jacob Mares, Markus Kalkum, Yoko Fujita-Yamaguchi, Hsun Teresa Ku
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-024-80889-y
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author Janine C. Quijano
Honoka Natsuyama
Alonso Tapia
Karine Bagramyan
Jose A. Ortiz
Jacob Mares
Markus Kalkum
Yoko Fujita-Yamaguchi
Hsun Teresa Ku
author_facet Janine C. Quijano
Honoka Natsuyama
Alonso Tapia
Karine Bagramyan
Jose A. Ortiz
Jacob Mares
Markus Kalkum
Yoko Fujita-Yamaguchi
Hsun Teresa Ku
author_sort Janine C. Quijano
collection DOAJ
description Abstract Lectins are produced in almost all life forms, can interact with targets (glycans) in a cross-kingdom manner and have served as valuable tools for studying glycobiology. Previously, a bacterial lectin, named Streptomyces hemagglutinin (SHA), was found to agglutinate human type B erythrocytes. However, the binding of SHA to mammalian cell types other than human erythrocytes has not been explored. To address this, we produced a recombinant fusion protein, with the mCherry reporter protein proceeding the SHA protein (referred to as mCherry-SHA), and performed co-immunofluorescence staining analysis. We focused on the normal pancreas in this study because glycans on pancreatic cells have been associated with initiation and progression of pancreatic cancer, a deadly disease. We found that only acinar, but not ductal or endocrine cells were stained positively with mCherry-SHA from embryonic day (E) 18.5 to 35 weeks old mice; in contrast, E12.5 and E15.5 pancreas display minimal mCherry-SHA binding. In adult humans, mCherry-SHA also targeted acinar cells specifically; however, only tissue from blood type B donors, but not type A or O donors, showed positivity. Together, these results demonstrate that SHA can bind to normal murine and human pancreatic acinar cells and that SHA-binding glycans are developmentally regulated.
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spelling doaj-art-b1b75bac6d9c47f29df26e0234613e1b2025-01-26T12:28:20ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-024-80889-yA lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humansJanine C. Quijano0Honoka Natsuyama1Alonso Tapia2Karine Bagramyan3Jose A. Ortiz4Jacob Mares5Markus Kalkum6Yoko Fujita-Yamaguchi7Hsun Teresa Ku8Department of Translational Research and Cellular Therapeutics, Arthur Riggs Diabetes and Metabolism Research InstituteDepartment of Translational Research and Cellular Therapeutics, Arthur Riggs Diabetes and Metabolism Research InstituteThe Irell and Manella Graduate School of Biological SciencesDepartment of Immunology and Theranostics, Arthur Riggs Diabetes and Metabolism Research InstituteDepartment of Translational Research and Cellular Therapeutics, Arthur Riggs Diabetes and Metabolism Research InstituteDepartment of Translational Research and Cellular Therapeutics, Arthur Riggs Diabetes and Metabolism Research InstituteDepartment of Immunology and Theranostics, Arthur Riggs Diabetes and Metabolism Research InstituteDepartment of Diabetes Complications and Metabolism, Beckman Research Institute, Arthur Riggs Diabetes and Metabolism Research Institute, City of HopeDepartment of Translational Research and Cellular Therapeutics, Arthur Riggs Diabetes and Metabolism Research InstituteAbstract Lectins are produced in almost all life forms, can interact with targets (glycans) in a cross-kingdom manner and have served as valuable tools for studying glycobiology. Previously, a bacterial lectin, named Streptomyces hemagglutinin (SHA), was found to agglutinate human type B erythrocytes. However, the binding of SHA to mammalian cell types other than human erythrocytes has not been explored. To address this, we produced a recombinant fusion protein, with the mCherry reporter protein proceeding the SHA protein (referred to as mCherry-SHA), and performed co-immunofluorescence staining analysis. We focused on the normal pancreas in this study because glycans on pancreatic cells have been associated with initiation and progression of pancreatic cancer, a deadly disease. We found that only acinar, but not ductal or endocrine cells were stained positively with mCherry-SHA from embryonic day (E) 18.5 to 35 weeks old mice; in contrast, E12.5 and E15.5 pancreas display minimal mCherry-SHA binding. In adult humans, mCherry-SHA also targeted acinar cells specifically; however, only tissue from blood type B donors, but not type A or O donors, showed positivity. Together, these results demonstrate that SHA can bind to normal murine and human pancreatic acinar cells and that SHA-binding glycans are developmentally regulated.https://doi.org/10.1038/s41598-024-80889-yBacterial lectinStreptomyces sp. 27S5PancreasAcinar cells
spellingShingle Janine C. Quijano
Honoka Natsuyama
Alonso Tapia
Karine Bagramyan
Jose A. Ortiz
Jacob Mares
Markus Kalkum
Yoko Fujita-Yamaguchi
Hsun Teresa Ku
A lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans
Scientific Reports
Bacterial lectin
Streptomyces sp. 27S5
Pancreas
Acinar cells
title A lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans
title_full A lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans
title_fullStr A lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans
title_full_unstemmed A lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans
title_short A lectin produced by a Streptomyces species targets mammalian pancreatic acinar cells in mice and humans
title_sort lectin produced by a streptomyces species targets mammalian pancreatic acinar cells in mice and humans
topic Bacterial lectin
Streptomyces sp. 27S5
Pancreas
Acinar cells
url https://doi.org/10.1038/s41598-024-80889-y
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