Pullulan/Collagen Scaffolds Promote Chronic Wound Healing via Mesenchymal Stem Cells
This study investigated the development of Pullulan/Collagen nanofiber scaffolds integrated with mesenchymal stem cells (MSCs) to enhance chronic wound healing. The combination of these biopolymers aims to optimize the scaffold properties for cell growth, viability, and tissue regeneration. Material...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Micro |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8023/4/4/37 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850241804621840384 |
|---|---|
| author | Elçin Tören Adnan Ahmed Mazari |
| author_facet | Elçin Tören Adnan Ahmed Mazari |
| author_sort | Elçin Tören |
| collection | DOAJ |
| description | This study investigated the development of Pullulan/Collagen nanofiber scaffolds integrated with mesenchymal stem cells (MSCs) to enhance chronic wound healing. The combination of these biopolymers aims to optimize the scaffold properties for cell growth, viability, and tissue regeneration. Materials and Methods: Pullulan, Collagen, and Pullulan/Collagen composite nanofibers were fabricated using electrospinning. The fibers were characterized using scanning electron microscopy (SEM) to determine the fiber diameter, and Fourier-transform infrared spectroscopy (FTIR) was employed to assess the molecular interactions. Cell viability was evaluated using MSCs cultured on the scaffolds and apoptosis assays were conducted to assess cell health. Distilled water was used as the solvent to maximize biocompatibility. Results: SEM analysis revealed that Pullulan nanofibers exhibited a larger average diameter (274 ± 20 nm) compared to Collagen fibers (167.03 ± 40.04 nm), while the Pullulan/Collagen composite fibers averaged 280 ± 102 nm. FTIR confirmed the molecular interactions between Pullulan and Collagen. Regarding biocompatibility, the Pullulan/Collagen scaffold demonstrated superior cell viability at 99% compared to 91% for Pullulan alone. Apoptosis assays indicated significantly lower necrosis rates for the composite scaffold (1.29%) than for the Pullulan-only scaffolds (2.35%). Conclusion: The use of distilled water as a solvent played a critical role in increasing cell viability and facilitating healthy proliferation of MSCs without cellular damage. Additionally, the reduced platelet activation and macrophage activity (0.75-fold for both) further supported the biocompatibility of the Pullulan/Collagen scaffold, demonstrating its potential for tissue engineering and chronic wound healing applications. |
| format | Article |
| id | doaj-art-b1b3be351b6d474784b3cb82233ced4c |
| institution | OA Journals |
| issn | 2673-8023 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micro |
| spelling | doaj-art-b1b3be351b6d474784b3cb82233ced4c2025-08-20T02:00:29ZengMDPI AGMicro2673-80232024-10-014459962010.3390/micro4040037Pullulan/Collagen Scaffolds Promote Chronic Wound Healing via Mesenchymal Stem CellsElçin Tören0Adnan Ahmed Mazari1Faculty of Textile Engineering, Technical University of Liberec, 1402/2, 461 17 Liberec, Czech RepublicFaculty of Textile Engineering, Technical University of Liberec, 1402/2, 461 17 Liberec, Czech RepublicThis study investigated the development of Pullulan/Collagen nanofiber scaffolds integrated with mesenchymal stem cells (MSCs) to enhance chronic wound healing. The combination of these biopolymers aims to optimize the scaffold properties for cell growth, viability, and tissue regeneration. Materials and Methods: Pullulan, Collagen, and Pullulan/Collagen composite nanofibers were fabricated using electrospinning. The fibers were characterized using scanning electron microscopy (SEM) to determine the fiber diameter, and Fourier-transform infrared spectroscopy (FTIR) was employed to assess the molecular interactions. Cell viability was evaluated using MSCs cultured on the scaffolds and apoptosis assays were conducted to assess cell health. Distilled water was used as the solvent to maximize biocompatibility. Results: SEM analysis revealed that Pullulan nanofibers exhibited a larger average diameter (274 ± 20 nm) compared to Collagen fibers (167.03 ± 40.04 nm), while the Pullulan/Collagen composite fibers averaged 280 ± 102 nm. FTIR confirmed the molecular interactions between Pullulan and Collagen. Regarding biocompatibility, the Pullulan/Collagen scaffold demonstrated superior cell viability at 99% compared to 91% for Pullulan alone. Apoptosis assays indicated significantly lower necrosis rates for the composite scaffold (1.29%) than for the Pullulan-only scaffolds (2.35%). Conclusion: The use of distilled water as a solvent played a critical role in increasing cell viability and facilitating healthy proliferation of MSCs without cellular damage. Additionally, the reduced platelet activation and macrophage activity (0.75-fold for both) further supported the biocompatibility of the Pullulan/Collagen scaffold, demonstrating its potential for tissue engineering and chronic wound healing applications.https://www.mdpi.com/2673-8023/4/4/37pullulancollagenmesenchymal stem cells (MSCs)electrospinningchronic wound healingnanofiber wound dressing |
| spellingShingle | Elçin Tören Adnan Ahmed Mazari Pullulan/Collagen Scaffolds Promote Chronic Wound Healing via Mesenchymal Stem Cells Micro pullulan collagen mesenchymal stem cells (MSCs) electrospinning chronic wound healing nanofiber wound dressing |
| title | Pullulan/Collagen Scaffolds Promote Chronic Wound Healing via Mesenchymal Stem Cells |
| title_full | Pullulan/Collagen Scaffolds Promote Chronic Wound Healing via Mesenchymal Stem Cells |
| title_fullStr | Pullulan/Collagen Scaffolds Promote Chronic Wound Healing via Mesenchymal Stem Cells |
| title_full_unstemmed | Pullulan/Collagen Scaffolds Promote Chronic Wound Healing via Mesenchymal Stem Cells |
| title_short | Pullulan/Collagen Scaffolds Promote Chronic Wound Healing via Mesenchymal Stem Cells |
| title_sort | pullulan collagen scaffolds promote chronic wound healing via mesenchymal stem cells |
| topic | pullulan collagen mesenchymal stem cells (MSCs) electrospinning chronic wound healing nanofiber wound dressing |
| url | https://www.mdpi.com/2673-8023/4/4/37 |
| work_keys_str_mv | AT elcintoren pullulancollagenscaffoldspromotechronicwoundhealingviamesenchymalstemcells AT adnanahmedmazari pullulancollagenscaffoldspromotechronicwoundhealingviamesenchymalstemcells |