Establishing a universal IVRP method for quadrivalent HPV vaccines to replace in vivo potency tests
Abstract Several human papillomavirus (HPV) L1-based virus-like particle (VLP) vaccines are in development to meet future global vaccination needs. Type-specific monoclonal antibodies with good reactivity to all types of vaccines are urgently needed to evaluate vaccine potency. In this study, bindin...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-03-01
|
| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-025-01106-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850133277018423296 |
|---|---|
| author | Jinpan Hu Zijing Jia Meng Wang Lingling Nie Wangjun Fu Qingfeng Zhang Haiyang Qin Jianhui Nie Xiaoyu Xu Lingjie Xu Fengze Wang Yingping Chen Bo Xing Tao Li Danfeng Li Shaowei Li Ningshao Xia Xiangxi Wang Weijin Huang |
| author_facet | Jinpan Hu Zijing Jia Meng Wang Lingling Nie Wangjun Fu Qingfeng Zhang Haiyang Qin Jianhui Nie Xiaoyu Xu Lingjie Xu Fengze Wang Yingping Chen Bo Xing Tao Li Danfeng Li Shaowei Li Ningshao Xia Xiangxi Wang Weijin Huang |
| author_sort | Jinpan Hu |
| collection | DOAJ |
| description | Abstract Several human papillomavirus (HPV) L1-based virus-like particle (VLP) vaccines are in development to meet future global vaccination needs. Type-specific monoclonal antibodies with good reactivity to all types of vaccines are urgently needed to evaluate vaccine potency. In this study, binding activity, neutralizing activity, conformational sensitivity, immunodominance in human serum, and versatility were compared among antibodies. A broad-spectrum binding antibody (C4-F5-127) was selected as the capture antibody; four type-specific neutralizing antibodies (6-F5-77, 11-F5-187, 16-F5-196, and 18-F5-203) were selected as detection antibodies for HPV6, 11, 16, and 18, respectively. These antibodies formed a standardized and universal in vitro relative potency (IVRP) assay kit. High-resolution cryo-electron microscopy (cryo-EM) structures of HPV6-6-F5-77, HPV11-11-F5-187, HPV16-16-F5-196 and HPV18-18-F5-203 complexes define the location and nature of epitopes, revealing serotype specific binding modes and neutralization mechanisms. The IVRP results were correlated with potency data from mouse models, offering an efficient alternative to in vivo potency experiments. |
| format | Article |
| id | doaj-art-b1ae507f91bf4ebfacd0717e5cec922c |
| institution | OA Journals |
| issn | 2059-0105 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj-art-b1ae507f91bf4ebfacd0717e5cec922c2025-08-20T02:32:00ZengNature Portfolionpj Vaccines2059-01052025-03-0110111110.1038/s41541-025-01106-zEstablishing a universal IVRP method for quadrivalent HPV vaccines to replace in vivo potency testsJinpan Hu0Zijing Jia1Meng Wang2Lingling Nie3Wangjun Fu4Qingfeng Zhang5Haiyang Qin6Jianhui Nie7Xiaoyu Xu8Lingjie Xu9Fengze Wang10Yingping Chen11Bo Xing12Tao Li13Danfeng Li14Shaowei Li15Ningshao Xia16Xiangxi Wang17Weijin Huang18National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen UniversityCAS Key Laboratory of Infection and Immunity, National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of SciencesDivision of HIV/AIDS and Sexually Transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC)Division of HIV/AIDS and Sexually Transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC)CAS Key Laboratory of Infection and Immunity, National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of SciencesDivision of HIV/AIDS and Sexually Transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC)Division of HIV/AIDS and Sexually Transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC)Division of HIV/AIDS and Sexually Transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC)Vazyme Biotech Co. LtdVazyme Biotech Co. LtdVazyme Biotech Co. LtdDepartment of Immunization Program, Zhejiang Provincial Center for Disease Control and PreventionDepartment of Immunization Program, Zhejiang Provincial Center for Disease Control and PreventionDivision of HIV/AIDS and Sexually Transmitted Virus Vaccines, National Institutes for Food and Drug Control (NIFDC)Guangxi Institute for Drug ControlNational Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen UniversityNational Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen UniversityCAS Key Laboratory of Infection and Immunity, National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of SciencesNational Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Public Health, Xiamen UniversityAbstract Several human papillomavirus (HPV) L1-based virus-like particle (VLP) vaccines are in development to meet future global vaccination needs. Type-specific monoclonal antibodies with good reactivity to all types of vaccines are urgently needed to evaluate vaccine potency. In this study, binding activity, neutralizing activity, conformational sensitivity, immunodominance in human serum, and versatility were compared among antibodies. A broad-spectrum binding antibody (C4-F5-127) was selected as the capture antibody; four type-specific neutralizing antibodies (6-F5-77, 11-F5-187, 16-F5-196, and 18-F5-203) were selected as detection antibodies for HPV6, 11, 16, and 18, respectively. These antibodies formed a standardized and universal in vitro relative potency (IVRP) assay kit. High-resolution cryo-electron microscopy (cryo-EM) structures of HPV6-6-F5-77, HPV11-11-F5-187, HPV16-16-F5-196 and HPV18-18-F5-203 complexes define the location and nature of epitopes, revealing serotype specific binding modes and neutralization mechanisms. The IVRP results were correlated with potency data from mouse models, offering an efficient alternative to in vivo potency experiments.https://doi.org/10.1038/s41541-025-01106-z |
| spellingShingle | Jinpan Hu Zijing Jia Meng Wang Lingling Nie Wangjun Fu Qingfeng Zhang Haiyang Qin Jianhui Nie Xiaoyu Xu Lingjie Xu Fengze Wang Yingping Chen Bo Xing Tao Li Danfeng Li Shaowei Li Ningshao Xia Xiangxi Wang Weijin Huang Establishing a universal IVRP method for quadrivalent HPV vaccines to replace in vivo potency tests npj Vaccines |
| title | Establishing a universal IVRP method for quadrivalent HPV vaccines to replace in vivo potency tests |
| title_full | Establishing a universal IVRP method for quadrivalent HPV vaccines to replace in vivo potency tests |
| title_fullStr | Establishing a universal IVRP method for quadrivalent HPV vaccines to replace in vivo potency tests |
| title_full_unstemmed | Establishing a universal IVRP method for quadrivalent HPV vaccines to replace in vivo potency tests |
| title_short | Establishing a universal IVRP method for quadrivalent HPV vaccines to replace in vivo potency tests |
| title_sort | establishing a universal ivrp method for quadrivalent hpv vaccines to replace in vivo potency tests |
| url | https://doi.org/10.1038/s41541-025-01106-z |
| work_keys_str_mv | AT jinpanhu establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT zijingjia establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT mengwang establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT linglingnie establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT wangjunfu establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT qingfengzhang establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT haiyangqin establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT jianhuinie establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT xiaoyuxu establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT lingjiexu establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT fengzewang establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT yingpingchen establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT boxing establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT taoli establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT danfengli establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT shaoweili establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT ningshaoxia establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT xiangxiwang establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests AT weijinhuang establishingauniversalivrpmethodforquadrivalenthpvvaccinestoreplaceinvivopotencytests |