Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain
We investigated the potential of antibody-vectorialized superparamagnetic iron oxide (SPIO) particles as cellular specific magnetic resonance contrast agents to image lymphocyte populations within the central nervous system (CNS), with the final goal of obtaining a reliable tool for noninvasively de...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2012-03-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2011.00032 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841561876306067456 |
|---|---|
| author | Alessandro Luchetti Davide Milani Francesca Ruffini Rossella Galli Andrea Falini Angelo Quattrini Giuseppe Scotti Giancarlo Comi Gianvito Martino Roberto Furlan Letterio S. Politi |
| author_facet | Alessandro Luchetti Davide Milani Francesca Ruffini Rossella Galli Andrea Falini Angelo Quattrini Giuseppe Scotti Giancarlo Comi Gianvito Martino Roberto Furlan Letterio S. Politi |
| author_sort | Alessandro Luchetti |
| collection | DOAJ |
| description | We investigated the potential of antibody-vectorialized superparamagnetic iron oxide (SPIO) particles as cellular specific magnetic resonance contrast agents to image lymphocyte populations within the central nervous system (CNS), with the final goal of obtaining a reliable tool for noninvasively detecting and tracking specific cellular populations in vivo. We used superparamagnetic particles bound to a monoclonal antibody. The particle is the contrast agent, by means of its T 2 * relaxation properties; the antibody is the targeting vector, responsible for homing the particle to target a surface antigen. To investigate the efficiency of particle vectorialization by these antibodies, we compared two types of antibody-vectorialized CD3-specific particles in vivo. We successfully employed vectorialized SPIO particles to image B220 + cells in a murine model of B-cell lymphoma. Likewise, we were able to identify CD3 + infiltrates in a murine model of multiple sclerosis. The specificity of the technique was confirmed by immunohistochemistry and electron microscopy of corresponding sections. Our findings suggest that indirect binding of the antibody to a streptavidinated particle allows for enhanced particle vectorialization compared to covalent binding of the antibody to the particle. |
| format | Article |
| id | doaj-art-b1a9a6a71347412db8c7e06e9da944cf |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2012-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-b1a9a6a71347412db8c7e06e9da944cf2025-01-03T01:22:47ZengSAGE PublishingMolecular Imaging1536-01212012-03-011110.2310/7290.2011.0003210.2310_7290.2011.00032Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse BrainAlessandro LuchettiDavide MilaniFrancesca RuffiniRossella GalliAndrea FaliniAngelo QuattriniGiuseppe ScottiGiancarlo ComiGianvito MartinoRoberto FurlanLetterio S. PolitiWe investigated the potential of antibody-vectorialized superparamagnetic iron oxide (SPIO) particles as cellular specific magnetic resonance contrast agents to image lymphocyte populations within the central nervous system (CNS), with the final goal of obtaining a reliable tool for noninvasively detecting and tracking specific cellular populations in vivo. We used superparamagnetic particles bound to a monoclonal antibody. The particle is the contrast agent, by means of its T 2 * relaxation properties; the antibody is the targeting vector, responsible for homing the particle to target a surface antigen. To investigate the efficiency of particle vectorialization by these antibodies, we compared two types of antibody-vectorialized CD3-specific particles in vivo. We successfully employed vectorialized SPIO particles to image B220 + cells in a murine model of B-cell lymphoma. Likewise, we were able to identify CD3 + infiltrates in a murine model of multiple sclerosis. The specificity of the technique was confirmed by immunohistochemistry and electron microscopy of corresponding sections. Our findings suggest that indirect binding of the antibody to a streptavidinated particle allows for enhanced particle vectorialization compared to covalent binding of the antibody to the particle.https://doi.org/10.2310/7290.2011.00032 |
| spellingShingle | Alessandro Luchetti Davide Milani Francesca Ruffini Rossella Galli Andrea Falini Angelo Quattrini Giuseppe Scotti Giancarlo Comi Gianvito Martino Roberto Furlan Letterio S. Politi Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain Molecular Imaging |
| title | Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain |
| title_full | Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain |
| title_fullStr | Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain |
| title_full_unstemmed | Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain |
| title_short | Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain |
| title_sort | monoclonal antibodies conjugated with superparamagnetic iron oxide particles allow magnetic resonance imaging detection of lymphocytes in the mouse brain |
| url | https://doi.org/10.2310/7290.2011.00032 |
| work_keys_str_mv | AT alessandroluchetti monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain AT davidemilani monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain AT francescaruffini monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain AT rossellagalli monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain AT andreafalini monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain AT angeloquattrini monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain AT giuseppescotti monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain AT giancarlocomi monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain AT gianvitomartino monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain AT robertofurlan monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain AT letteriospoliti monoclonalantibodiesconjugatedwithsuperparamagneticironoxideparticlesallowmagneticresonanceimagingdetectionoflymphocytesinthemousebrain |