Identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory mice
Inclusion body nephropathy (IBN) and kidney fibrosis in aged immunodeficient mice and, to lesser extent, in immunocompetent mice have been recently linked to infection of mouse kidney parvovirus (MKPV), also known as murine chapparvovirus (MuCPV). Knowledge about its prevalence and the complete geno...
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1798288 |
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| author | Zhongming Ge Sebastian E. Carrasco Yan Feng Vasudevan Bakthavatchalu Damodaran Annamalai Robin Kramer Sureshkumar Muthupalani James G. Fox |
| author_facet | Zhongming Ge Sebastian E. Carrasco Yan Feng Vasudevan Bakthavatchalu Damodaran Annamalai Robin Kramer Sureshkumar Muthupalani James G. Fox |
| author_sort | Zhongming Ge |
| collection | DOAJ |
| description | Inclusion body nephropathy (IBN) and kidney fibrosis in aged immunodeficient mice and, to lesser extent, in immunocompetent mice have been recently linked to infection of mouse kidney parvovirus (MKPV), also known as murine chapparvovirus (MuCPV). Knowledge about its prevalence and the complete genome sequence of more MKPV strains is essential for understanding phylogenetic relationships and pathogenicity among MKPV strains. In the present study using PCR and genome walking, we determined the complete 4440-nucleotide genome of a new MKPV strain, namely MIT-WI1, which was identified in IBN-affected Il2rg−/–Rag2−/– c-Kit W–sh/W–sh mice housed in the vivarium at Whitehead Institute for Biomedical Research (WI). The overall nucleotide (>94%) and deduced amino acid sequences (>98%) of p10, p15, NS1 (replicase), NS2 and VP1 (capsid protein) within the MIT-WI1 genome, are closely related to MKPV/MuCPV strains described in laboratory and wild Mus musculus mice. In addition, PCR and qPCR assays using newly designed primers conserved among the known MKPV/MuCPV genomes were developed and utilized to assess MKPV status in selected laboratory mice. MKPV was also detected in immunodeficient (NSG) and immunocompetent (Crl:CD1(ICR), UTXflox) mouse strains/stocks. The abundance of the MKPV genome copies was significantly correlated with the severity of IBN. Our data indicate that MKPV is present in selected mouse strains/stocks, and provides new insights into the genome evolution of MKPV. |
| format | Article |
| id | doaj-art-b1a193be5b8d4b45a35db8ed16ff91bf |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
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| series | Emerging Microbes and Infections |
| spelling | doaj-art-b1a193be5b8d4b45a35db8ed16ff91bf2025-08-20T02:26:37ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911814182310.1080/22221751.2020.1798288Identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory miceZhongming Ge0Sebastian E. Carrasco1Yan Feng2Vasudevan Bakthavatchalu3Damodaran Annamalai4Robin Kramer5Sureshkumar Muthupalani6James G. Fox7Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USADivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USADivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USADivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USADivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USADivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USADivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USADivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USAInclusion body nephropathy (IBN) and kidney fibrosis in aged immunodeficient mice and, to lesser extent, in immunocompetent mice have been recently linked to infection of mouse kidney parvovirus (MKPV), also known as murine chapparvovirus (MuCPV). Knowledge about its prevalence and the complete genome sequence of more MKPV strains is essential for understanding phylogenetic relationships and pathogenicity among MKPV strains. In the present study using PCR and genome walking, we determined the complete 4440-nucleotide genome of a new MKPV strain, namely MIT-WI1, which was identified in IBN-affected Il2rg−/–Rag2−/– c-Kit W–sh/W–sh mice housed in the vivarium at Whitehead Institute for Biomedical Research (WI). The overall nucleotide (>94%) and deduced amino acid sequences (>98%) of p10, p15, NS1 (replicase), NS2 and VP1 (capsid protein) within the MIT-WI1 genome, are closely related to MKPV/MuCPV strains described in laboratory and wild Mus musculus mice. In addition, PCR and qPCR assays using newly designed primers conserved among the known MKPV/MuCPV genomes were developed and utilized to assess MKPV status in selected laboratory mice. MKPV was also detected in immunodeficient (NSG) and immunocompetent (Crl:CD1(ICR), UTXflox) mouse strains/stocks. The abundance of the MKPV genome copies was significantly correlated with the severity of IBN. Our data indicate that MKPV is present in selected mouse strains/stocks, and provides new insights into the genome evolution of MKPV.https://www.tandfonline.com/doi/10.1080/22221751.2020.1798288MKPVgenome organizationIBNPCR diagnosis |
| spellingShingle | Zhongming Ge Sebastian E. Carrasco Yan Feng Vasudevan Bakthavatchalu Damodaran Annamalai Robin Kramer Sureshkumar Muthupalani James G. Fox Identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory mice Emerging Microbes and Infections MKPV genome organization IBN PCR diagnosis |
| title | Identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory mice |
| title_full | Identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory mice |
| title_fullStr | Identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory mice |
| title_full_unstemmed | Identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory mice |
| title_short | Identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory mice |
| title_sort | identification of a new strain of mouse kidney parvovirus associated with inclusion body nephropathy in immunocompromised laboratory mice |
| topic | MKPV genome organization IBN PCR diagnosis |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1798288 |
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