Metagenomic analysis reveals the presence of prunus virus I in diseased Clematis vitalba: first record of this virus in Italy
Prunus virus I (PrVI) was detected for the first time in Clematis vitalba in Italy using high-throughput sequencing and the complete genome of this isolate, named Clv-1, was assembled and characterized. The results of the bioinformatic analyses were further validated with RT-PCR assays using PrVI-sp...
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Firenze University Press
2024-02-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/14925 |
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| author | Giuseppe PARRELLA Elisa TROIANO Anna MIGNANO |
| author_facet | Giuseppe PARRELLA Elisa TROIANO Anna MIGNANO |
| author_sort | Giuseppe PARRELLA |
| collection | DOAJ |
| description | Prunus virus I (PrVI) was detected for the first time in Clematis vitalba in Italy using high-throughput sequencing and the complete genome of this isolate, named Clv-1, was assembled and characterized. The results of the bioinformatic analyses were further validated with RT-PCR assays using PrVI-specific primers and Sanger dideoxy sequencing. The Clv-1 genome included three RNA segments of nucleotide lengths of 3468 (RNA1), 2892 (RNA2) and 2225 (RNA3), with five predicted open reading frames. Phylogenetic analyses showed close relationships with other PrVI isolates from different geographical origins, including European and non-European countries. This new pathogen record extends the information on the geographical distribution of PrVI, and possibly reflects the international movement of infected clematis germplasm due to global trade. Further surveys on the presence and distribution of PrVI in weeds and crops, such as the two PrVI hosts sweet cherry and peach, are required in the countries where PrVI has been detected. |
| format | Article |
| id | doaj-art-b1a008b2c90140a5bec2beaaab9d8cc4 |
| institution | DOAJ |
| issn | 0031-9465 1593-2095 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj-art-b1a008b2c90140a5bec2beaaab9d8cc42025-08-20T03:05:25ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952024-02-016313710.36253/phyto-1492513754Metagenomic analysis reveals the presence of prunus virus I in diseased Clematis vitalba: first record of this virus in ItalyGiuseppe PARRELLA0https://orcid.org/0000-0002-0412-4014Elisa TROIANO1https://orcid.org/0000-0001-7755-4915Anna MIGNANO2https://orcid.org/0000-0001-5365-7186Institute for Sustainable Plant Protection of the National Research Council (IPSP-CNR), Piazzale Enrico Fermi 1 – 80055 Portici (NA)Institute for Sustainable Plant Protection of the National Research Council (IPSP-CNR), Piazzale Enrico Fermi 1 – 80055 Portici (NA)Institute for Sustainable Plant Protection of the National Research Council (IPSP-CNR), Piazzale Enrico Fermi 1 – 80055 Portici (NA)Prunus virus I (PrVI) was detected for the first time in Clematis vitalba in Italy using high-throughput sequencing and the complete genome of this isolate, named Clv-1, was assembled and characterized. The results of the bioinformatic analyses were further validated with RT-PCR assays using PrVI-specific primers and Sanger dideoxy sequencing. The Clv-1 genome included three RNA segments of nucleotide lengths of 3468 (RNA1), 2892 (RNA2) and 2225 (RNA3), with five predicted open reading frames. Phylogenetic analyses showed close relationships with other PrVI isolates from different geographical origins, including European and non-European countries. This new pathogen record extends the information on the geographical distribution of PrVI, and possibly reflects the international movement of infected clematis germplasm due to global trade. Further surveys on the presence and distribution of PrVI in weeds and crops, such as the two PrVI hosts sweet cherry and peach, are required in the countries where PrVI has been detected.https://oajournals.fupress.net/index.php/pm/article/view/14925prvibromoviridaeclematis spphigh-throughput sequencingemerging viruses |
| spellingShingle | Giuseppe PARRELLA Elisa TROIANO Anna MIGNANO Metagenomic analysis reveals the presence of prunus virus I in diseased Clematis vitalba: first record of this virus in Italy Phytopathologia Mediterranea prvi bromoviridae clematis spp high-throughput sequencing emerging viruses |
| title | Metagenomic analysis reveals the presence of prunus virus I in diseased Clematis vitalba: first record of this virus in Italy |
| title_full | Metagenomic analysis reveals the presence of prunus virus I in diseased Clematis vitalba: first record of this virus in Italy |
| title_fullStr | Metagenomic analysis reveals the presence of prunus virus I in diseased Clematis vitalba: first record of this virus in Italy |
| title_full_unstemmed | Metagenomic analysis reveals the presence of prunus virus I in diseased Clematis vitalba: first record of this virus in Italy |
| title_short | Metagenomic analysis reveals the presence of prunus virus I in diseased Clematis vitalba: first record of this virus in Italy |
| title_sort | metagenomic analysis reveals the presence of prunus virus i in diseased clematis vitalba first record of this virus in italy |
| topic | prvi bromoviridae clematis spp high-throughput sequencing emerging viruses |
| url | https://oajournals.fupress.net/index.php/pm/article/view/14925 |
| work_keys_str_mv | AT giuseppeparrella metagenomicanalysisrevealsthepresenceofprunusvirusiindiseasedclematisvitalbafirstrecordofthisvirusinitaly AT elisatroiano metagenomicanalysisrevealsthepresenceofprunusvirusiindiseasedclematisvitalbafirstrecordofthisvirusinitaly AT annamignano metagenomicanalysisrevealsthepresenceofprunusvirusiindiseasedclematisvitalbafirstrecordofthisvirusinitaly |