The gut microbiota in mice with erythropoietin—induced abdominal aortic aneurysm
Background In recent years, a novel animal abdominal aortic aneurysm (AAA) model was established by administering erythropoietin (EPO) to wild-type (WT) mice. However, the influence of EPO on the murine fecal microbiota remains uninvestigated. Therefore, this study aims to explore the potential asso...
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PeerJ Inc.
2025-04-01
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| author | Xinyi Lyu Mingjun Jiang Jiahao Shi Qi Liu Xilian Liu Yulan Li Shu-Qin Ding Xianpeng Dai |
| author_facet | Xinyi Lyu Mingjun Jiang Jiahao Shi Qi Liu Xilian Liu Yulan Li Shu-Qin Ding Xianpeng Dai |
| author_sort | Xinyi Lyu |
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| description | Background In recent years, a novel animal abdominal aortic aneurysm (AAA) model was established by administering erythropoietin (EPO) to wild-type (WT) mice. However, the influence of EPO on the murine fecal microbiota remains uninvestigated. Therefore, this study aims to explore the potential association between gut microbiota changes and AAA development in this model. Methods and results Adult male C57BL/6 mice were used to establish the AAA model by intraperitoneal administration of recombinant human EPO at a dosage of 10,000 IU/kg daily for 28 consecutive days. Hematoxylin and eosin (H&E) and Elastin Van Gieson (EVG) staining revealed that EPO administration increased aortic wall thickness and diameter, accompanied by enhanced degradation of the elastic lamina. The 16S rRNA—sequencing data were deposited in the Sequence Read Archive (PRJNA1172300). LEfSe analysis revealed that Akkermansia, Lawsonibacter, Clostridium, and Neglectibacter were significantly associated with EPO-induced AAA development, while Lactobacillus, Alistipes, Limosilactobacillus, and Eisenbergiella showed significant negative correlations. Analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) prediction module revealed significant differences in metabolic pathways between the two groups, including alanine, aspartate and glutamate metabolism; cysteine and methionine metabolism; pyrimidine metabolism; carbon metabolism; ABC transporters; and oxidative phosphorylation pathways. Conclusions EPO-induced gut dysbiosis, particularly changes in Akkermansia, Lactobacillus, and Alistipes abundance, may contribute to AAA formation via inflammation, oxidative stress, and metabolic dysfunction. While this model advances AAA research, its limitations underscore the need for human validation and mechanistic studies. Future work should prioritize multi-omics integration and cross-model comparisons to unravel the complex microbiota-AAA axis. |
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| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-b19829eb866e4605ada5bec2b3ca32d72025-08-20T02:16:46ZengPeerJ Inc.PeerJ2167-83592025-04-0113e1922210.7717/peerj.19222The gut microbiota in mice with erythropoietin—induced abdominal aortic aneurysmXinyi Lyu0Mingjun Jiang1Jiahao Shi2Qi Liu3Xilian Liu4Yulan Li5Shu-Qin Ding6Xianpeng Dai7Department of Vascular Surgery, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaDepartment of Vascular Surgery, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaDepartment of Vascular Surgery, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaDepartment of Vascular Surgery, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaDepartment of Endocrinology and Metabolism, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaDepartment of Endocrinology and Metabolism, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaClinical Laboratory, The First Affiliated Hospital of Bengbu Medical University, Anhui University, Bengbu, Anhui, ChinaDepartment of Vascular Surgery, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaBackground In recent years, a novel animal abdominal aortic aneurysm (AAA) model was established by administering erythropoietin (EPO) to wild-type (WT) mice. However, the influence of EPO on the murine fecal microbiota remains uninvestigated. Therefore, this study aims to explore the potential association between gut microbiota changes and AAA development in this model. Methods and results Adult male C57BL/6 mice were used to establish the AAA model by intraperitoneal administration of recombinant human EPO at a dosage of 10,000 IU/kg daily for 28 consecutive days. Hematoxylin and eosin (H&E) and Elastin Van Gieson (EVG) staining revealed that EPO administration increased aortic wall thickness and diameter, accompanied by enhanced degradation of the elastic lamina. The 16S rRNA—sequencing data were deposited in the Sequence Read Archive (PRJNA1172300). LEfSe analysis revealed that Akkermansia, Lawsonibacter, Clostridium, and Neglectibacter were significantly associated with EPO-induced AAA development, while Lactobacillus, Alistipes, Limosilactobacillus, and Eisenbergiella showed significant negative correlations. Analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) prediction module revealed significant differences in metabolic pathways between the two groups, including alanine, aspartate and glutamate metabolism; cysteine and methionine metabolism; pyrimidine metabolism; carbon metabolism; ABC transporters; and oxidative phosphorylation pathways. Conclusions EPO-induced gut dysbiosis, particularly changes in Akkermansia, Lactobacillus, and Alistipes abundance, may contribute to AAA formation via inflammation, oxidative stress, and metabolic dysfunction. While this model advances AAA research, its limitations underscore the need for human validation and mechanistic studies. Future work should prioritize multi-omics integration and cross-model comparisons to unravel the complex microbiota-AAA axis.https://peerj.com/articles/19222.pdfAbdominal aortic aneurysmErythropoietinGut microbiota16S rRNA-sequencing |
| spellingShingle | Xinyi Lyu Mingjun Jiang Jiahao Shi Qi Liu Xilian Liu Yulan Li Shu-Qin Ding Xianpeng Dai The gut microbiota in mice with erythropoietin—induced abdominal aortic aneurysm PeerJ Abdominal aortic aneurysm Erythropoietin Gut microbiota 16S rRNA-sequencing |
| title | The gut microbiota in mice with erythropoietin—induced abdominal aortic aneurysm |
| title_full | The gut microbiota in mice with erythropoietin—induced abdominal aortic aneurysm |
| title_fullStr | The gut microbiota in mice with erythropoietin—induced abdominal aortic aneurysm |
| title_full_unstemmed | The gut microbiota in mice with erythropoietin—induced abdominal aortic aneurysm |
| title_short | The gut microbiota in mice with erythropoietin—induced abdominal aortic aneurysm |
| title_sort | gut microbiota in mice with erythropoietin induced abdominal aortic aneurysm |
| topic | Abdominal aortic aneurysm Erythropoietin Gut microbiota 16S rRNA-sequencing |
| url | https://peerj.com/articles/19222.pdf |
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