A Role for Bcl-2 in Notch1-Dependent Transcription in Thymic Lymphoma Cells
Notch1 is a transcription factor important for T-cell development. Notch1 is active in double negative (DN) thymocytes, while being depressed in double positive (DP) thymocytes. Synchronously, the expression of Bcl-2 becomes downregulated during the transition from DN to DP thymocytes. We previously...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Advances in Hematology |
| Online Access: | http://dx.doi.org/10.1155/2012/435241 |
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| author | Ronit Vogt Sionov Shlomit Kfir-Erenfeld Rachel Spokoini Eitan Yefenof |
| author_facet | Ronit Vogt Sionov Shlomit Kfir-Erenfeld Rachel Spokoini Eitan Yefenof |
| author_sort | Ronit Vogt Sionov |
| collection | DOAJ |
| description | Notch1 is a transcription factor important for T-cell development. Notch1 is active in double negative (DN) thymocytes, while being depressed in double positive (DP) thymocytes. Synchronously, the expression of Bcl-2 becomes downregulated during the transition from DN to DP thymocytes. We previously observed that overexpression of an intracellular active Notch1 (ICN) in Bcl-2-positive 2B4 T cells leads to the transcription of Notch1-regulated genes. However, these genes were not induced in Bcl-2-negative DP PD1.6 thymic lymphoma cells overexpressing ICN. Here we show that, when Bcl-2 is simultaneously introduced into these cells, Notch-regulated genes are transcribed. Only in the presence of both Bcl-2 and ICN, PD1.6 thymic lymphoma cells become resistant to glucocorticoid (GC)-induced apoptosis. Our data suggest that Bcl-2 plays a role in modulating Notch1 function in T cells. |
| format | Article |
| id | doaj-art-b197bdca6f164c65bf9649458fb0497c |
| institution | OA Journals |
| issn | 1687-9104 1687-9112 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Hematology |
| spelling | doaj-art-b197bdca6f164c65bf9649458fb0497c2025-08-20T02:23:35ZengWileyAdvances in Hematology1687-91041687-91122012-01-01201210.1155/2012/435241435241A Role for Bcl-2 in Notch1-Dependent Transcription in Thymic Lymphoma CellsRonit Vogt Sionov0Shlomit Kfir-Erenfeld1Rachel Spokoini2Eitan Yefenof3The Lautenberg Center for General and Tumor Immunology, Institute for Medical Research Israel-Canada (IMRIC), Hebrew University-Hadassah Medical School, Jerusalem 91120, IsraelThe Lautenberg Center for General and Tumor Immunology, Institute for Medical Research Israel-Canada (IMRIC), Hebrew University-Hadassah Medical School, Jerusalem 91120, IsraelThe Lautenberg Center for General and Tumor Immunology, Institute for Medical Research Israel-Canada (IMRIC), Hebrew University-Hadassah Medical School, Jerusalem 91120, IsraelThe Lautenberg Center for General and Tumor Immunology, Institute for Medical Research Israel-Canada (IMRIC), Hebrew University-Hadassah Medical School, Jerusalem 91120, IsraelNotch1 is a transcription factor important for T-cell development. Notch1 is active in double negative (DN) thymocytes, while being depressed in double positive (DP) thymocytes. Synchronously, the expression of Bcl-2 becomes downregulated during the transition from DN to DP thymocytes. We previously observed that overexpression of an intracellular active Notch1 (ICN) in Bcl-2-positive 2B4 T cells leads to the transcription of Notch1-regulated genes. However, these genes were not induced in Bcl-2-negative DP PD1.6 thymic lymphoma cells overexpressing ICN. Here we show that, when Bcl-2 is simultaneously introduced into these cells, Notch-regulated genes are transcribed. Only in the presence of both Bcl-2 and ICN, PD1.6 thymic lymphoma cells become resistant to glucocorticoid (GC)-induced apoptosis. Our data suggest that Bcl-2 plays a role in modulating Notch1 function in T cells.http://dx.doi.org/10.1155/2012/435241 |
| spellingShingle | Ronit Vogt Sionov Shlomit Kfir-Erenfeld Rachel Spokoini Eitan Yefenof A Role for Bcl-2 in Notch1-Dependent Transcription in Thymic Lymphoma Cells Advances in Hematology |
| title | A Role for Bcl-2 in Notch1-Dependent Transcription in Thymic Lymphoma Cells |
| title_full | A Role for Bcl-2 in Notch1-Dependent Transcription in Thymic Lymphoma Cells |
| title_fullStr | A Role for Bcl-2 in Notch1-Dependent Transcription in Thymic Lymphoma Cells |
| title_full_unstemmed | A Role for Bcl-2 in Notch1-Dependent Transcription in Thymic Lymphoma Cells |
| title_short | A Role for Bcl-2 in Notch1-Dependent Transcription in Thymic Lymphoma Cells |
| title_sort | role for bcl 2 in notch1 dependent transcription in thymic lymphoma cells |
| url | http://dx.doi.org/10.1155/2012/435241 |
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