De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Male and Female Gonads in <i>Acrossocheilus parallens</i>
<i>Acrossocheilus parallens</i> has become an important commercial aquaculture species in southern China due to its high nutritional content and ornamental value. However, at present, there is very little research on its gonad development and reproductive regulation, which has restricted...
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MDPI AG
2025-03-01
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| Online Access: | https://www.mdpi.com/2076-2615/15/6/806 |
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| author | Weiqian Liang Lanyuan Liu Dingxian Chen Kaifeng Wang Shengyue Lin Weijian Chen Sixun Li Binhua Deng Qiang Li Chong Han |
| author_facet | Weiqian Liang Lanyuan Liu Dingxian Chen Kaifeng Wang Shengyue Lin Weijian Chen Sixun Li Binhua Deng Qiang Li Chong Han |
| author_sort | Weiqian Liang |
| collection | DOAJ |
| description | <i>Acrossocheilus parallens</i> has become an important commercial aquaculture species in southern China due to its high nutritional content and ornamental value. However, at present, there is very little research on its gonad development and reproductive regulation, which has restricted the development of its aquaculture industry. In this research, the gonadal transcriptome sequencing data of female and male <i>A. parallens</i> were first analyzed and compared. A total of 67,251 unigenes were successfully assembled and a total of 34,069 unigenes were annotated. After the comparative transcriptome analysis, a sum of 14,514 differentially expressed genes (DEGs) were identified between the male and female gonads, with 9111 having significantly high expression in the testes and 5403 having high expression in the ovaries. Additionally, 82 DEGs related to reproduction, gonad development and differentiation in the gonads were identified and the differential expression profiles of partial genes were further validated using real-time fluorescence quantitative PCR. These results provide basic data for further research on the functions of the genes and pathways related to sex differentiation and gonad development in <i>A. parallens</i>. |
| format | Article |
| id | doaj-art-b192840e36ad42f19f2d407e8a4c66a4 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-b192840e36ad42f19f2d407e8a4c66a42025-08-20T03:43:50ZengMDPI AGAnimals2076-26152025-03-0115680610.3390/ani15060806De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Male and Female Gonads in <i>Acrossocheilus parallens</i>Weiqian Liang0Lanyuan Liu1Dingxian Chen2Kaifeng Wang3Shengyue Lin4Weijian Chen5Sixun Li6Binhua Deng7Qiang Li8Chong Han9School of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaSchool of Life Sciences, Guangzhou University, Guangzhou 510006, China<i>Acrossocheilus parallens</i> has become an important commercial aquaculture species in southern China due to its high nutritional content and ornamental value. However, at present, there is very little research on its gonad development and reproductive regulation, which has restricted the development of its aquaculture industry. In this research, the gonadal transcriptome sequencing data of female and male <i>A. parallens</i> were first analyzed and compared. A total of 67,251 unigenes were successfully assembled and a total of 34,069 unigenes were annotated. After the comparative transcriptome analysis, a sum of 14,514 differentially expressed genes (DEGs) were identified between the male and female gonads, with 9111 having significantly high expression in the testes and 5403 having high expression in the ovaries. Additionally, 82 DEGs related to reproduction, gonad development and differentiation in the gonads were identified and the differential expression profiles of partial genes were further validated using real-time fluorescence quantitative PCR. These results provide basic data for further research on the functions of the genes and pathways related to sex differentiation and gonad development in <i>A. parallens</i>.https://www.mdpi.com/2076-2615/15/6/806<i>Acrossocheilus parallens</i>transcriptome analysissex differentiationgonad developmentreproduction |
| spellingShingle | Weiqian Liang Lanyuan Liu Dingxian Chen Kaifeng Wang Shengyue Lin Weijian Chen Sixun Li Binhua Deng Qiang Li Chong Han De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Male and Female Gonads in <i>Acrossocheilus parallens</i> Animals <i>Acrossocheilus parallens</i> transcriptome analysis sex differentiation gonad development reproduction |
| title | De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Male and Female Gonads in <i>Acrossocheilus parallens</i> |
| title_full | De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Male and Female Gonads in <i>Acrossocheilus parallens</i> |
| title_fullStr | De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Male and Female Gonads in <i>Acrossocheilus parallens</i> |
| title_full_unstemmed | De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Male and Female Gonads in <i>Acrossocheilus parallens</i> |
| title_short | De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Male and Female Gonads in <i>Acrossocheilus parallens</i> |
| title_sort | de novo assembly characterization and comparative transcriptome analysis of the mature male and female gonads in i acrossocheilus parallens i |
| topic | <i>Acrossocheilus parallens</i> transcriptome analysis sex differentiation gonad development reproduction |
| url | https://www.mdpi.com/2076-2615/15/6/806 |
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