Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time
Summary: Lymph nodes regulate immunity and maintain fluid balance in health and disease. Here, we present a protocol that uses normothermic perfusion to sustain patient-derived lymph nodes ex vivo for up to 24 h to study their structure and function. We describe steps for setting up both thermoregul...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000309 |
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| author | Amy Llewellyn Rachel Barrow-McGee Julia Stevenson Jasmine Gore Kalnisha Naidoo |
| author_facet | Amy Llewellyn Rachel Barrow-McGee Julia Stevenson Jasmine Gore Kalnisha Naidoo |
| author_sort | Amy Llewellyn |
| collection | DOAJ |
| description | Summary: Lymph nodes regulate immunity and maintain fluid balance in health and disease. Here, we present a protocol that uses normothermic perfusion to sustain patient-derived lymph nodes ex vivo for up to 24 h to study their structure and function. We describe steps for setting up both thermoregulatory and perfusion circuits, cannulating human lymph nodes, and perfusion. This protocol can be used to study how human lymph nodes change in cancer and other diseases, and/or in response to perturbations, including drugs.For complete details on the use and execution of this protocol, please refer to Barrow-McGee et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-b180fb526534438b86970af5ce287682 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-b180fb526534438b86970af5ce2876822025-02-07T04:48:20ZengElsevierSTAR Protocols2666-16672025-03-0161103624Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real timeAmy Llewellyn0Rachel Barrow-McGee1Julia Stevenson2Jasmine Gore3Kalnisha Naidoo4Translational Pathology, Comprehensive Cancer Centre, School of Cancer and Pharmaceutical Sciences, King’s College London, London, UKToby Robins Breast Cancer Now Research Centre, Breast Cancer Research Division, The Institute of Cancer Research, London, UKKing’s Health Partners Cancer Biobank, Guy’s Comprehensive Cancer Centre, London, UKTranslational Pathology, Comprehensive Cancer Centre, School of Cancer and Pharmaceutical Sciences, King’s College London, London, UKTranslational Pathology, Comprehensive Cancer Centre, School of Cancer and Pharmaceutical Sciences, King’s College London, London, UK; Department of Cellular Pathology, King’s College Hospital, London, UK; Corresponding authorSummary: Lymph nodes regulate immunity and maintain fluid balance in health and disease. Here, we present a protocol that uses normothermic perfusion to sustain patient-derived lymph nodes ex vivo for up to 24 h to study their structure and function. We describe steps for setting up both thermoregulatory and perfusion circuits, cannulating human lymph nodes, and perfusion. This protocol can be used to study how human lymph nodes change in cancer and other diseases, and/or in response to perturbations, including drugs.For complete details on the use and execution of this protocol, please refer to Barrow-McGee et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000309CancerImmunologyTissue Engineering |
| spellingShingle | Amy Llewellyn Rachel Barrow-McGee Julia Stevenson Jasmine Gore Kalnisha Naidoo Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time STAR Protocols Cancer Immunology Tissue Engineering |
| title | Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time |
| title_full | Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time |
| title_fullStr | Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time |
| title_full_unstemmed | Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time |
| title_short | Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time |
| title_sort | protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time |
| topic | Cancer Immunology Tissue Engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000309 |
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