Detection of <i>Ehrlichia</i> spp. and <i>Theileria</i> spp. in <i>Hyalomma anatolicum</i> ticks collected in Tajikistan
The objectives of our study were to survey the prevalence of genetic markers for Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. in Hyalomma anatolicum ticks collected in southwestern Tajikistan and to perform sequencing and phylogenetic analysis of fragments of the...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2020-03-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2480 |
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| author | M. Yu. Kartashov Yu. V. Kononova I. D. Petrova N. L. Tupota T. P. Mikryukova V. A. Ternovoi F. H. Tishkova V. B. Loktev |
| author_facet | M. Yu. Kartashov Yu. V. Kononova I. D. Petrova N. L. Tupota T. P. Mikryukova V. A. Ternovoi F. H. Tishkova V. B. Loktev |
| author_sort | M. Yu. Kartashov |
| collection | DOAJ |
| description | The objectives of our study were to survey the prevalence of genetic markers for Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. in Hyalomma anatolicum ticks collected in southwestern Tajikistan and to perform sequencing and phylogenetic analysis of fragments of the 16S rRNA gene and groESL ope-ron from Ehrlichia spp. and fragments of the 18S rRNA gene of Theileria spp. detected in H. anatolicum ticks. Hyalomma anatolicum ticks collected in the Tursunzade and Rudaki districts of Tajikistan were tested for DNA of Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. by PCR with specific primers. The amplified fragments were sequenced and analyzed. DNA of Ehrlichia spp. (3.3 %) and Theileria spp. (3.3 %) was detected only in H. anatolicum ticks collected from the Rudaki district, and DNA of Ehrlichia spp. (0.7 %) was found in H. anatolicum ticks from the Tursunzade district. Sequence analysis of fragments of the 16S rRNA gene and groESL operon from Ehrlichia spp. revealed high similarity to Ehrlichia spp. The Tajik isolates of Theileria spp. were genotyped as Theileriaannulata based on the analysis of 18S rRNA gene sequences. The phylogenetic analysis demonstrates that Ehrlichia spp. isolates are highly similar to Ehrlichia spp. circulating in China and Brazil. The isolate Tajikistan-5 is closely related to the putative novel species Ehrlichia mineirensis. The Tajik isolates of Theileria spp. were clustered with T. annulata isolates from Turkey, Iran, Pakistan, and China by phylogenetic analyses. |
| format | Article |
| id | doaj-art-b1773324f2ef4c7faa1f83010169feed |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2020-03-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-b1773324f2ef4c7faa1f83010169feed2025-02-01T09:58:08ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592020-03-01241555910.18699/VJ20.5951022Detection of <i>Ehrlichia</i> spp. and <i>Theileria</i> spp. in <i>Hyalomma anatolicum</i> ticks collected in TajikistanM. Yu. Kartashov0Yu. V. Kononova1I. D. Petrova2N. L. Tupota3T. P. Mikryukova4V. A. Ternovoi5F. H. Tishkova6V. B. Loktev7State Research Center for Virology and Biotechnology “Vector"; Novosibirsk State UniversityState Research Center for Virology and Biotechnology “Vector"; Tomsk State UniversityState Research Center for Virology and Biotechnology “Vector"State Research Center for Virology and Biotechnology “Vector"State Research Center for Virology and Biotechnology “Vector"; Tomsk State UniversityState Research Center for Virology and Biotechnology “Vector"; Tomsk State UniversityTajik Research Institute of Preventive MedicineState Research Center for Virology and Biotechnology “Vector"; Novosibirsk State University; Tomsk State University; Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesThe objectives of our study were to survey the prevalence of genetic markers for Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. in Hyalomma anatolicum ticks collected in southwestern Tajikistan and to perform sequencing and phylogenetic analysis of fragments of the 16S rRNA gene and groESL ope-ron from Ehrlichia spp. and fragments of the 18S rRNA gene of Theileria spp. detected in H. anatolicum ticks. Hyalomma anatolicum ticks collected in the Tursunzade and Rudaki districts of Tajikistan were tested for DNA of Rickettsia spp., Ehrlichia spp., Anaplasma spp., Babesia spp., and Theileria spp. by PCR with specific primers. The amplified fragments were sequenced and analyzed. DNA of Ehrlichia spp. (3.3 %) and Theileria spp. (3.3 %) was detected only in H. anatolicum ticks collected from the Rudaki district, and DNA of Ehrlichia spp. (0.7 %) was found in H. anatolicum ticks from the Tursunzade district. Sequence analysis of fragments of the 16S rRNA gene and groESL operon from Ehrlichia spp. revealed high similarity to Ehrlichia spp. The Tajik isolates of Theileria spp. were genotyped as Theileriaannulata based on the analysis of 18S rRNA gene sequences. The phylogenetic analysis demonstrates that Ehrlichia spp. isolates are highly similar to Ehrlichia spp. circulating in China and Brazil. The isolate Tajikistan-5 is closely related to the putative novel species Ehrlichia mineirensis. The Tajik isolates of Theileria spp. were clustered with T. annulata isolates from Turkey, Iran, Pakistan, and China by phylogenetic analyses.https://vavilov.elpub.ru/jour/article/view/2480hyalomma anatolicumtick-borne infectionsehrlichia spp.theileria spp.tajikistan |
| spellingShingle | M. Yu. Kartashov Yu. V. Kononova I. D. Petrova N. L. Tupota T. P. Mikryukova V. A. Ternovoi F. H. Tishkova V. B. Loktev Detection of <i>Ehrlichia</i> spp. and <i>Theileria</i> spp. in <i>Hyalomma anatolicum</i> ticks collected in Tajikistan Вавиловский журнал генетики и селекции hyalomma anatolicum tick-borne infections ehrlichia spp. theileria spp. tajikistan |
| title | Detection of <i>Ehrlichia</i> spp. and <i>Theileria</i> spp. in <i>Hyalomma anatolicum</i> ticks collected in Tajikistan |
| title_full | Detection of <i>Ehrlichia</i> spp. and <i>Theileria</i> spp. in <i>Hyalomma anatolicum</i> ticks collected in Tajikistan |
| title_fullStr | Detection of <i>Ehrlichia</i> spp. and <i>Theileria</i> spp. in <i>Hyalomma anatolicum</i> ticks collected in Tajikistan |
| title_full_unstemmed | Detection of <i>Ehrlichia</i> spp. and <i>Theileria</i> spp. in <i>Hyalomma anatolicum</i> ticks collected in Tajikistan |
| title_short | Detection of <i>Ehrlichia</i> spp. and <i>Theileria</i> spp. in <i>Hyalomma anatolicum</i> ticks collected in Tajikistan |
| title_sort | detection of i ehrlichia i spp and i theileria i spp in i hyalomma anatolicum i ticks collected in tajikistan |
| topic | hyalomma anatolicum tick-borne infections ehrlichia spp. theileria spp. tajikistan |
| url | https://vavilov.elpub.ru/jour/article/view/2480 |
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