Rpv34: the noval Plasmopara viticola resistance locus in 'Moldova'

Downy mildew (DM), caused by Plasmopara viticola, is one of the most serious diseases that affects grape production worldwide. Utilizing host resistance, commonly known as 'R' genes, is considered the most effective way to cure the disease. The identification of the R gene in well-known ta...

Full description

Saved in:
Bibliographic Details
Main Authors: Yan Sun, Bohan Yang, Minmin Li, Changjiang Liu, Yonggang Yin, Nan Jia, Xinyu Wang, Qingming Zeng, Yong Guo, Yingjie Wang, Guohong Wu, Qinqin Gu, Jianfeng Lou, Peining Fu, Bin Han
Format: Article
Language:English
Published: Maximum Academic Press 2024-01-01
Series:Fruit Research
Subjects:
Online Access:https://www.maxapress.com/article/doi/10.48130/frures-0024-0017
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850199928849039360
author Yan Sun
Bohan Yang
Minmin Li
Changjiang Liu
Yonggang Yin
Nan Jia
Xinyu Wang
Qingming Zeng
Yong Guo
Yingjie Wang
Guohong Wu
Qinqin Gu
Jianfeng Lou
Peining Fu
Bin Han
author_facet Yan Sun
Bohan Yang
Minmin Li
Changjiang Liu
Yonggang Yin
Nan Jia
Xinyu Wang
Qingming Zeng
Yong Guo
Yingjie Wang
Guohong Wu
Qinqin Gu
Jianfeng Lou
Peining Fu
Bin Han
author_sort Yan Sun
collection DOAJ
description Downy mildew (DM), caused by Plasmopara viticola, is one of the most serious diseases that affects grape production worldwide. Utilizing host resistance, commonly known as 'R' genes, is considered the most effective way to cure the disease. The identification of the R gene in well-known table grape cultivars is conducive to breeding varieties with better quality. In this study, a population of F1 with 246 progenies was obtained from the cross of two widely grown table grapes 'Moldova' and 'Shine Muscat', in which 'Moldova' appeared resistant to P. viticola resistance. According to the sequencing data from GBTS, a high-density genetic map containing 826 SNPs, which spanned 1,515.99 cM, was constructed. Through the analysis of MapQTL and GWAS, a resistant locus for P. viticola was mapped, located at 27.534−30.729 Mbp in chr18, and was named Rpv34. A total of 21 Resistance Gene Analogues (RGA) distributed in three clusters were identified in this locus. A SNP marker chr18_29062596 was proved to be highly associated with resistance to P. viticola. This work will improve the mechanism of resistance understanding of P. viticola in grapevine and facilitate the use of resistant germplasm in breeding.
format Article
id doaj-art-b17231fc2fa84548a5166dcd2fdc3c09
institution OA Journals
issn 2769-4615
language English
publishDate 2024-01-01
publisher Maximum Academic Press
record_format Article
series Fruit Research
spelling doaj-art-b17231fc2fa84548a5166dcd2fdc3c092025-08-20T02:12:30ZengMaximum Academic PressFruit Research2769-46152024-01-01411910.48130/frures-0024-0017frures-0024-0017Rpv34: the noval Plasmopara viticola resistance locus in 'Moldova'Yan Sun0Bohan Yang1Minmin Li2Changjiang Liu3Yonggang Yin4Nan Jia5Xinyu Wang6Qingming Zeng7Yong Guo8Yingjie Wang9Guohong Wu10Qinqin Gu11Jianfeng Lou12Peining Fu13Bin Han14Changli Research Institute of Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, Hebei, ChinaCenter for Viticulture and Enology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaChangli Research Institute of Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, Hebei, ChinaChangli Research Institute of Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, Hebei, ChinaChangli Research Institute of Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, Hebei, ChinaChangli Research Institute of Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, Hebei, ChinaChangli Research Institute of Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, Hebei, ChinaChangli Research Institute of Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, Hebei, ChinaChangli Research Institute of Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, Hebei, ChinaChangli Research Institute of Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, Hebei, ChinaXinjiang Uygur Autonomous Region Grape and Melon Research Institute, Turpan, Xinjiang Uygur Autonomous 838200, ChinaShanghai Agricultural Technology Extension and Service Center, Shanghai 201103, ChinaShanghai Agricultural Technology Extension and Service Center, Shanghai 201103, ChinaCenter for Viticulture and Enology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaChangli Research Institute of Fruit Trees, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, Hebei, ChinaDowny mildew (DM), caused by Plasmopara viticola, is one of the most serious diseases that affects grape production worldwide. Utilizing host resistance, commonly known as 'R' genes, is considered the most effective way to cure the disease. The identification of the R gene in well-known table grape cultivars is conducive to breeding varieties with better quality. In this study, a population of F1 with 246 progenies was obtained from the cross of two widely grown table grapes 'Moldova' and 'Shine Muscat', in which 'Moldova' appeared resistant to P. viticola resistance. According to the sequencing data from GBTS, a high-density genetic map containing 826 SNPs, which spanned 1,515.99 cM, was constructed. Through the analysis of MapQTL and GWAS, a resistant locus for P. viticola was mapped, located at 27.534−30.729 Mbp in chr18, and was named Rpv34. A total of 21 Resistance Gene Analogues (RGA) distributed in three clusters were identified in this locus. A SNP marker chr18_29062596 was proved to be highly associated with resistance to P. viticola. This work will improve the mechanism of resistance understanding of P. viticola in grapevine and facilitate the use of resistant germplasm in breeding.https://www.maxapress.com/article/doi/10.48130/frures-0024-0017grapevineplasmopara viticola resistanceqtl mappinggwas
spellingShingle Yan Sun
Bohan Yang
Minmin Li
Changjiang Liu
Yonggang Yin
Nan Jia
Xinyu Wang
Qingming Zeng
Yong Guo
Yingjie Wang
Guohong Wu
Qinqin Gu
Jianfeng Lou
Peining Fu
Bin Han
Rpv34: the noval Plasmopara viticola resistance locus in 'Moldova'
Fruit Research
grapevine
plasmopara viticola resistance
qtl mapping
gwas
title Rpv34: the noval Plasmopara viticola resistance locus in 'Moldova'
title_full Rpv34: the noval Plasmopara viticola resistance locus in 'Moldova'
title_fullStr Rpv34: the noval Plasmopara viticola resistance locus in 'Moldova'
title_full_unstemmed Rpv34: the noval Plasmopara viticola resistance locus in 'Moldova'
title_short Rpv34: the noval Plasmopara viticola resistance locus in 'Moldova'
title_sort rpv34 the noval plasmopara viticola resistance locus in moldova
topic grapevine
plasmopara viticola resistance
qtl mapping
gwas
url https://www.maxapress.com/article/doi/10.48130/frures-0024-0017
work_keys_str_mv AT yansun rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT bohanyang rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT minminli rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT changjiangliu rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT yonggangyin rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT nanjia rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT xinyuwang rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT qingmingzeng rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT yongguo rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT yingjiewang rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT guohongwu rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT qinqingu rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT jianfenglou rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT peiningfu rpv34thenovalplasmoparaviticolaresistancelocusinmoldova
AT binhan rpv34thenovalplasmoparaviticolaresistancelocusinmoldova