Antibodies directed to antigens secreted by murine epithelioid macrophages modulate BCG-induced granulomata
The authors have previously shown that epithelioid cells isolated from mice secrete a factor, called macrophage deactivating factor (MDF), that promptly deactivates superoxide release by activated macrophages and neutrophils. In this paper some biological properties of a polyclonal rat antiserum dir...
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| Format: | Article |
| Language: | English |
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Wiley
1993-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935193000316 |
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| author | Mario Mariano Tania Aguiar Celia R. W. Carneiro Edilberto Postol Venânclo A. F. Alves Ricardo Ribeiro-dos-Santos |
| author_facet | Mario Mariano Tania Aguiar Celia R. W. Carneiro Edilberto Postol Venânclo A. F. Alves Ricardo Ribeiro-dos-Santos |
| author_sort | Mario Mariano |
| collection | DOAJ |
| description | The authors have previously shown that epithelioid cells isolated from mice secrete a factor, called macrophage deactivating factor (MDF), that promptly deactivates superoxide release by activated macrophages and neutrophils. In this paper some biological properties of a polyclonal rat antiserum directed to MDF and other substances secreted by these cells are described. The immunoglobulin fraction of this antiserum reacted, by immunocytochemical methods, with epitopes in the cell membrane of macrophages adherent to coverslips subcutaneously implanted for 14 days; but not for 5 days. It also reacted with antigens within and outside cells in BCG-induced granulomas. This antiserum blocked completely the macrophage deactivating activity of epithelioid cell culture supernatants. Anti-IL-10 monoclonal antibody, did not block MDF activity. The administration of the immunoglobulin fraction from immunized rats to C5 deficient mice bearing BCG-induced granulomatas in the footpad, significantly reduced the size of the lesions. A marked necrosis of inflammatory cells and mononuclear cells phagocyting debris of necrotic cells were observed in these lesions. |
| format | Article |
| id | doaj-art-b16cc829ba11441cb2dc8c846320cba6 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1993-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b16cc829ba11441cb2dc8c846320cba62025-02-03T01:25:56ZengWileyMediators of Inflammation0962-93511466-18611993-01-012322923310.1155/S0962935193000316Antibodies directed to antigens secreted by murine epithelioid macrophages modulate BCG-induced granulomataMario Mariano0Tania Aguiar1Celia R. W. Carneiro2Edilberto Postol3Venânclo A. F. Alves4Ricardo Ribeiro-dos-Santos5Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, SP, BrazilDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo, SP, BrazilLudwig Institute for Cancer Research, São Paulo branch, São Paulo, SP, BrazilLudwig Institute for Cancer Research, São Paulo branch, São Paulo, SP, BrazilDepartment of Pathology, Adolpho Lutz Institute, SP, BrazilDepartment of Biochemistry and Molecular Biology, Oswaldo Cruz Institute, Rio de Janeiro, RJ, BrazilThe authors have previously shown that epithelioid cells isolated from mice secrete a factor, called macrophage deactivating factor (MDF), that promptly deactivates superoxide release by activated macrophages and neutrophils. In this paper some biological properties of a polyclonal rat antiserum directed to MDF and other substances secreted by these cells are described. The immunoglobulin fraction of this antiserum reacted, by immunocytochemical methods, with epitopes in the cell membrane of macrophages adherent to coverslips subcutaneously implanted for 14 days; but not for 5 days. It also reacted with antigens within and outside cells in BCG-induced granulomas. This antiserum blocked completely the macrophage deactivating activity of epithelioid cell culture supernatants. Anti-IL-10 monoclonal antibody, did not block MDF activity. The administration of the immunoglobulin fraction from immunized rats to C5 deficient mice bearing BCG-induced granulomatas in the footpad, significantly reduced the size of the lesions. A marked necrosis of inflammatory cells and mononuclear cells phagocyting debris of necrotic cells were observed in these lesions.http://dx.doi.org/10.1155/S0962935193000316 |
| spellingShingle | Mario Mariano Tania Aguiar Celia R. W. Carneiro Edilberto Postol Venânclo A. F. Alves Ricardo Ribeiro-dos-Santos Antibodies directed to antigens secreted by murine epithelioid macrophages modulate BCG-induced granulomata Mediators of Inflammation |
| title | Antibodies directed to antigens secreted by murine epithelioid macrophages modulate BCG-induced granulomata |
| title_full | Antibodies directed to antigens secreted by murine epithelioid macrophages modulate BCG-induced granulomata |
| title_fullStr | Antibodies directed to antigens secreted by murine epithelioid macrophages modulate BCG-induced granulomata |
| title_full_unstemmed | Antibodies directed to antigens secreted by murine epithelioid macrophages modulate BCG-induced granulomata |
| title_short | Antibodies directed to antigens secreted by murine epithelioid macrophages modulate BCG-induced granulomata |
| title_sort | antibodies directed to antigens secreted by murine epithelioid macrophages modulate bcg induced granulomata |
| url | http://dx.doi.org/10.1155/S0962935193000316 |
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