Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation.
Emerging studies hint at the roles of autophagy-related proteins in various cellular processes. To understand if autophagy-related proteins influence genome stability, we sought to examine a cohort of 35 autophagy mutants in Saccharomyces cerevisiae. We observe cells lacking Atg11 show poor mitotic...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-04-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.3003069 |
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| author | Md Hashim Reza Rashi Aggarwal Jigyasa Verma Nitesh Kumar Podh Ratul Chowdhury Gunjan Mehta Ravi Manjithaya Kaustuv Sanyal |
| author_facet | Md Hashim Reza Rashi Aggarwal Jigyasa Verma Nitesh Kumar Podh Ratul Chowdhury Gunjan Mehta Ravi Manjithaya Kaustuv Sanyal |
| author_sort | Md Hashim Reza |
| collection | DOAJ |
| description | Emerging studies hint at the roles of autophagy-related proteins in various cellular processes. To understand if autophagy-related proteins influence genome stability, we sought to examine a cohort of 35 autophagy mutants in Saccharomyces cerevisiae. We observe cells lacking Atg11 show poor mitotic stability of minichromosomes. Single-molecule tracking assays and live cell microscopy reveal that Atg11 molecules dynamically localize to the spindle pole bodies (SPBs) in a microtubule (MT)-dependent manner. Loss of Atg11 leads to a delayed cell cycle progression. Such cells accumulate at metaphase at an elevated temperature that is relieved when the spindle assembly checkpoint (SAC) is inactivated. Indeed, atg11∆ cells have stabilized securin levels, that prevent anaphase onset. Ipl1-mediated activation of SAC also confirms that atg11∆ mutants are defective in chromosome biorientation. Atg11 functions in the Kar9-dependent spindle positioning pathway. Stabilized Clb4 levels in atg11∆ cells suggest that Atg11 maintains Kar9 asymmetry by facilitating proper dynamic instability of astral microtubules (aMTs). Loss of Spc72 asymmetry contributes to non-random SPB inheritance in atg11∆ cells. Overall, this study uncovers an essential non-canonical role of Atg11 in the MT-mediated process of chromosome segregation. |
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| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj-art-b169b7ee534f4fbfa2dfe1492623fef72025-08-20T02:26:27ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852025-04-01234e300306910.1371/journal.pbio.3003069Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation.Md Hashim RezaRashi AggarwalJigyasa VermaNitesh Kumar PodhRatul ChowdhuryGunjan MehtaRavi ManjithayaKaustuv SanyalEmerging studies hint at the roles of autophagy-related proteins in various cellular processes. To understand if autophagy-related proteins influence genome stability, we sought to examine a cohort of 35 autophagy mutants in Saccharomyces cerevisiae. We observe cells lacking Atg11 show poor mitotic stability of minichromosomes. Single-molecule tracking assays and live cell microscopy reveal that Atg11 molecules dynamically localize to the spindle pole bodies (SPBs) in a microtubule (MT)-dependent manner. Loss of Atg11 leads to a delayed cell cycle progression. Such cells accumulate at metaphase at an elevated temperature that is relieved when the spindle assembly checkpoint (SAC) is inactivated. Indeed, atg11∆ cells have stabilized securin levels, that prevent anaphase onset. Ipl1-mediated activation of SAC also confirms that atg11∆ mutants are defective in chromosome biorientation. Atg11 functions in the Kar9-dependent spindle positioning pathway. Stabilized Clb4 levels in atg11∆ cells suggest that Atg11 maintains Kar9 asymmetry by facilitating proper dynamic instability of astral microtubules (aMTs). Loss of Spc72 asymmetry contributes to non-random SPB inheritance in atg11∆ cells. Overall, this study uncovers an essential non-canonical role of Atg11 in the MT-mediated process of chromosome segregation.https://doi.org/10.1371/journal.pbio.3003069 |
| spellingShingle | Md Hashim Reza Rashi Aggarwal Jigyasa Verma Nitesh Kumar Podh Ratul Chowdhury Gunjan Mehta Ravi Manjithaya Kaustuv Sanyal Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation. PLoS Biology |
| title | Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation. |
| title_full | Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation. |
| title_fullStr | Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation. |
| title_full_unstemmed | Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation. |
| title_short | Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation. |
| title_sort | autophagy related protein atg11 is essential for microtubule mediated chromosome segregation |
| url | https://doi.org/10.1371/journal.pbio.3003069 |
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