Comparative prospective study on the clinical utility of G‐banding and next‐generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan
Abstract Purpose To compare the clinical utility of G‐banding and next‐generation sequencing (NGS) for chromosomal analysis of products of conception (POC), a crucial tool for detecting fetal chromosomal abnormalities which are major causes of miscarriage and stillbirth. Methods We evaluated the cli...
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| Format: | Article |
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Wiley
2025-01-01
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| Series: | Reproductive Medicine and Biology |
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| Online Access: | https://doi.org/10.1002/rmb2.12655 |
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| author | Hidemine Honda Tsuyoshi Takiuchi Mika Handa Nao Wakui Saori Tsuji Takeshi Goto Shota Suzuki Fumie Saji Tatsuya Miyake Sakae Goto Satomi Okamura Tomomi Yamada Michiko Kodama Tadashi Kimura |
| author_facet | Hidemine Honda Tsuyoshi Takiuchi Mika Handa Nao Wakui Saori Tsuji Takeshi Goto Shota Suzuki Fumie Saji Tatsuya Miyake Sakae Goto Satomi Okamura Tomomi Yamada Michiko Kodama Tadashi Kimura |
| author_sort | Hidemine Honda |
| collection | DOAJ |
| description | Abstract Purpose To compare the clinical utility of G‐banding and next‐generation sequencing (NGS) for chromosomal analysis of products of conception (POC), a crucial tool for detecting fetal chromosomal abnormalities which are major causes of miscarriage and stillbirth. Methods We evaluated the clinical utility of both techniques in a prospective analysis of 40 patients who experienced miscarriages or stillbirths between 6 and 36 weeks of gestation under Advanced Medical Care A in Japan. Both methods were applied to the same POC samples. The primary outcome was the proportion of patients with a presumed cause of miscarriage or stillbirth among all submitted samples. Results NGS presumed the cause in 75.0% (30/40) of cases, significantly outperforming G‐banding's 42.5% (17/40) (p < 0.01). G‐banding could analyze 67.5% (27/40) of the samples owing to culture failure, whereas NGS successfully analyzed all samples (100%, 40/40) (p < 0.01). Among the successfully analyzed samples, NGS presumed the cause in 70.3% (19/27) of cases, compared with 62.9% (17/27) for G‐banding (p = 0.31). For miscarriages before 12 weeks, NGS presumed the cause in 73.5% (25/34) of cases, significantly higher than the 44.1% (15/34) (p < 0.01) presumed using G‐banding. Conclusions These results highlight the superior efficacy of NGS over G‐banding for presuming causes of miscarriage or stillbirth. |
| format | Article |
| id | doaj-art-b169367fefcb493197871d408c4017e4 |
| institution | Kabale University |
| issn | 1445-5781 1447-0578 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj-art-b169367fefcb493197871d408c4017e42025-08-20T06:16:47ZengWileyReproductive Medicine and Biology1445-57811447-05782025-01-01241n/an/a10.1002/rmb2.12655Comparative prospective study on the clinical utility of G‐banding and next‐generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in JapanHidemine Honda0Tsuyoshi Takiuchi1Mika Handa2Nao Wakui3Saori Tsuji4Takeshi Goto5Shota Suzuki6Fumie Saji7Tatsuya Miyake8Sakae Goto9Satomi Okamura10Tomomi Yamada11Michiko Kodama12Tadashi Kimura13Department of Obstetrics and Gynecology The University of Osaka Suita Osaka JapanDepartment of Obstetrics and Gynecology The University of Osaka Suita Osaka JapanDepartment of Obstetrics and Gynecology The University of Osaka Suita Osaka JapanDepartment of Obstetrics and Gynecology The University of Osaka Suita Osaka JapanDepartment of Obstetrics and Gynecology The University of Osaka Suita Osaka JapanDepartment of Obstetrics and Gynecology The University of Osaka Suita Osaka JapanDepartment of Obstetrics and Gynecology The University of Osaka Suita Osaka JapanDepartment of Obstetrics and Gynecology The University of Osaka Suita Osaka JapanDepartment of Obstetrics and Gynecology The University of Osaka Suita Osaka JapanGoto Ladies Clinic Takatsuki Osaka JapanDepartment of Medical Innovation Osaka University Hospital Suita Osaka JapanDepartment of Medical Innovation Osaka University Hospital Suita Osaka JapanDepartment of Obstetrics and Gynecology The University of Osaka Suita Osaka JapanGraduate School of Medicine Division of Medicine Sakai City Hospital Sakai Osaka JapanAbstract Purpose To compare the clinical utility of G‐banding and next‐generation sequencing (NGS) for chromosomal analysis of products of conception (POC), a crucial tool for detecting fetal chromosomal abnormalities which are major causes of miscarriage and stillbirth. Methods We evaluated the clinical utility of both techniques in a prospective analysis of 40 patients who experienced miscarriages or stillbirths between 6 and 36 weeks of gestation under Advanced Medical Care A in Japan. Both methods were applied to the same POC samples. The primary outcome was the proportion of patients with a presumed cause of miscarriage or stillbirth among all submitted samples. Results NGS presumed the cause in 75.0% (30/40) of cases, significantly outperforming G‐banding's 42.5% (17/40) (p < 0.01). G‐banding could analyze 67.5% (27/40) of the samples owing to culture failure, whereas NGS successfully analyzed all samples (100%, 40/40) (p < 0.01). Among the successfully analyzed samples, NGS presumed the cause in 70.3% (19/27) of cases, compared with 62.9% (17/27) for G‐banding (p = 0.31). For miscarriages before 12 weeks, NGS presumed the cause in 73.5% (25/34) of cases, significantly higher than the 44.1% (15/34) (p < 0.01) presumed using G‐banding. Conclusions These results highlight the superior efficacy of NGS over G‐banding for presuming causes of miscarriage or stillbirth.https://doi.org/10.1002/rmb2.12655fetal chromosomal abnormalitiesG‐bandingmiscarriagenext‐generation sequencingproducts of conception |
| spellingShingle | Hidemine Honda Tsuyoshi Takiuchi Mika Handa Nao Wakui Saori Tsuji Takeshi Goto Shota Suzuki Fumie Saji Tatsuya Miyake Sakae Goto Satomi Okamura Tomomi Yamada Michiko Kodama Tadashi Kimura Comparative prospective study on the clinical utility of G‐banding and next‐generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan Reproductive Medicine and Biology fetal chromosomal abnormalities G‐banding miscarriage next‐generation sequencing products of conception |
| title | Comparative prospective study on the clinical utility of G‐banding and next‐generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan |
| title_full | Comparative prospective study on the clinical utility of G‐banding and next‐generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan |
| title_fullStr | Comparative prospective study on the clinical utility of G‐banding and next‐generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan |
| title_full_unstemmed | Comparative prospective study on the clinical utility of G‐banding and next‐generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan |
| title_short | Comparative prospective study on the clinical utility of G‐banding and next‐generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan |
| title_sort | comparative prospective study on the clinical utility of g banding and next generation sequencing for chromosomal analysis of products of conception under advanced medical care a in japan |
| topic | fetal chromosomal abnormalities G‐banding miscarriage next‐generation sequencing products of conception |
| url | https://doi.org/10.1002/rmb2.12655 |
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