Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials
We investigated the metabolome of the iron- and sulfur-oxidizing, extremely thermoacidophilic archaeon Metallosphaera sedula grown on mineral pyrite (FeS2). The extraction of organic materials from these microorganisms is a major challenge because of the tight contact and interaction between cells a...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1473270/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841555113273982976 |
|---|---|
| author | Sebastian V. Gfellner Sebastian V. Gfellner Cyril Colas Cyril Colas Cyril Colas Guillaume Gabant Guillaume Gabant Janina Groninga Martine Cadene Martine Cadene Tetyana Milojevic Tetyana Milojevic |
| author_facet | Sebastian V. Gfellner Sebastian V. Gfellner Cyril Colas Cyril Colas Cyril Colas Guillaume Gabant Guillaume Gabant Janina Groninga Martine Cadene Martine Cadene Tetyana Milojevic Tetyana Milojevic |
| author_sort | Sebastian V. Gfellner |
| collection | DOAJ |
| description | We investigated the metabolome of the iron- and sulfur-oxidizing, extremely thermoacidophilic archaeon Metallosphaera sedula grown on mineral pyrite (FeS2). The extraction of organic materials from these microorganisms is a major challenge because of the tight contact and interaction between cells and mineral materials. Therefore, we applied an improved protocol to break the microbial cells and separate their organic constituents from the mineral surface, to extract lipophilic compounds through liquid–liquid extraction, and performed metabolomics analyses using MALDI-TOF MS and UHPLC-UHR-Q/TOF. Using this approach, we identified several molecules involved in central carbon metabolism and in the modified Entner-Doudoroff pathway found in Archaea, sulfur metabolism-related compounds, and molecules involved in the adaptation of M. sedula to extreme environments, such as metal tolerance and acid resistance. Furthermore, we identified molecules involved in microbial interactions, i.e., cell surface interactions through biofilm formation and cell–cell interactions through quorum sensing, which relies on messenger molecules for microbial communication. Moreover, we successfully extracted and identified different saturated thiophene-bearing quinones using software for advanced compound identification (MetaboScape). These quinones are respiratory chain electron carriers in M. sedula, with biomarker potential for life detection in extreme environmental conditions. |
| format | Article |
| id | doaj-art-b165cfb0e7b243358ed92ad3626cff84 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-b165cfb0e7b243358ed92ad3626cff842025-01-08T06:11:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14732701473270Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materialsSebastian V. Gfellner0Sebastian V. Gfellner1Cyril Colas2Cyril Colas3Cyril Colas4Guillaume Gabant5Guillaume Gabant6Janina Groninga7Martine Cadene8Martine Cadene9Tetyana Milojevic10Tetyana Milojevic11UPR4301 Centre de Biophysique Moléculaire (CBM), Orléans, FranceUniversité d'Orléans, Orléans, FranceUPR4301 Centre de Biophysique Moléculaire (CBM), Orléans, FranceUniversité d'Orléans, Orléans, FranceUMR7311 Institut de Chimie Organique et Analytique (ICOA), Orléans, FranceUPR4301 Centre de Biophysique Moléculaire (CBM), Orléans, FranceUniversité d'Orléans, Orléans, FranceCenter for Marine Environmental Sciences, University of Bremen, Bremen, GermanyUPR4301 Centre de Biophysique Moléculaire (CBM), Orléans, FranceUniversité d'Orléans, Orléans, FranceUPR4301 Centre de Biophysique Moléculaire (CBM), Orléans, FranceUniversité d'Orléans, Orléans, FranceWe investigated the metabolome of the iron- and sulfur-oxidizing, extremely thermoacidophilic archaeon Metallosphaera sedula grown on mineral pyrite (FeS2). The extraction of organic materials from these microorganisms is a major challenge because of the tight contact and interaction between cells and mineral materials. Therefore, we applied an improved protocol to break the microbial cells and separate their organic constituents from the mineral surface, to extract lipophilic compounds through liquid–liquid extraction, and performed metabolomics analyses using MALDI-TOF MS and UHPLC-UHR-Q/TOF. Using this approach, we identified several molecules involved in central carbon metabolism and in the modified Entner-Doudoroff pathway found in Archaea, sulfur metabolism-related compounds, and molecules involved in the adaptation of M. sedula to extreme environments, such as metal tolerance and acid resistance. Furthermore, we identified molecules involved in microbial interactions, i.e., cell surface interactions through biofilm formation and cell–cell interactions through quorum sensing, which relies on messenger molecules for microbial communication. Moreover, we successfully extracted and identified different saturated thiophene-bearing quinones using software for advanced compound identification (MetaboScape). These quinones are respiratory chain electron carriers in M. sedula, with biomarker potential for life detection in extreme environmental conditions.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1473270/fullmetabolomicschemolithotrophsthiophene-bearing quinonesquorum sensingorganic extraction from minerals |
| spellingShingle | Sebastian V. Gfellner Sebastian V. Gfellner Cyril Colas Cyril Colas Cyril Colas Guillaume Gabant Guillaume Gabant Janina Groninga Martine Cadene Martine Cadene Tetyana Milojevic Tetyana Milojevic Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials Frontiers in Microbiology metabolomics chemolithotrophs thiophene-bearing quinones quorum sensing organic extraction from minerals |
| title | Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials |
| title_full | Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials |
| title_fullStr | Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials |
| title_full_unstemmed | Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials |
| title_short | Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials |
| title_sort | improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic archaea grown on mineral materials |
| topic | metabolomics chemolithotrophs thiophene-bearing quinones quorum sensing organic extraction from minerals |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1473270/full |
| work_keys_str_mv | AT sebastianvgfellner improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT sebastianvgfellner improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT cyrilcolas improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT cyrilcolas improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT cyrilcolas improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT guillaumegabant improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT guillaumegabant improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT janinagroninga improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT martinecadene improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT martinecadene improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT tetyanamilojevic improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials AT tetyanamilojevic improvedprotocolformetaboliteextractionandidentificationofrespiratoryquinonesinextremophilicarchaeagrownonmineralmaterials |