Effect of 25-hydroxycholecalciferol and high phytase doses on performance, vitamin D status, bone mineralization, and mechanistic target of rapamycin gene expression of broilers
The use of exogenous phytase and vitamin D3 metabolites such as 25-hydroxycholecalciferol (25-OH-D3) for poultry is well consolidated, but the potential for additive effects when supplementing both requires further investigation. This study investigated possible interactions between supplementation...
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Elsevier
2025-02-01
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| author | L.S. Bassi C.C.S. Martins C.A. Lozano-Poveda I.C. Dias S.G. Oliveira A. Maiorka |
| author_facet | L.S. Bassi C.C.S. Martins C.A. Lozano-Poveda I.C. Dias S.G. Oliveira A. Maiorka |
| author_sort | L.S. Bassi |
| collection | DOAJ |
| description | The use of exogenous phytase and vitamin D3 metabolites such as 25-hydroxycholecalciferol (25-OH-D3) for poultry is well consolidated, but the potential for additive effects when supplementing both requires further investigation. This study investigated possible interactions between supplementation of 25-OH-D3 and high doses of phytase for broilers fed Ca− and P-deficient diets. A total of 1 200 one-d-old male broiler chicks were randomly allocated from one of four dietary treatments in a 2 × 2 factorial arrangement: 600 or 2 000 phytase units (FYT)/kg and with or without the inclusion of 25-OH-D3 at 69 µg/kg, with 12 replicates of 25 broilers each. Two feeding phases were stablished (1-to-21 and 22-to-42 d) and all diets contained commercial levels of vitamin D3 (100 µg/kg) with total Ca and available P respectively set to 0.6 and 0.3%. Supplementation with 25-OH-D3 increased body weight gain (BWG) and reduced feed conversion ratio (FCR) from 1 to 21 d (P < 0.05), as well as increased BWG from 1 to 42 d (P < 0.05). Serum 25-OH-D3 levels at 21 and 42 d were increased with 25-OH-D3 (P < 0.001). Phytase did not affect growth performance from 1 to 21 d, but a higher dose (2 000 FYT/kg) reduced feed intake and FCR from 22 to 42 d compared to 600 FYT/kg, also reducing FCR in the total period (P < 0.05). The mRNA expression of mechanistic target of rapamycin in breast muscle assessed at 42 d was enhanced with 2 000 FYT/kg (P < 0.001). Bone weight, bone contents of ash, Ca, and P, and bone breaking strength of tibia bone measured at 42 d were not affected by any dietary treatment. Although both additives are known to improve dietary Ca and P utilization, there were no detected additive or synergic effects. The results suggested that the inclusion of 25-OH-D3 and phytase combined with regular vitamin D3 levels can help minimize losses of performance and bone mineralization of broilers fed Ca- and P-deficient diets. Furthermore, vitamin D status is refined with dietary 25-OH-D3 and potential improvements on breast meat yield can be obtained with a high phytase dose of 2 000 FYT/kg. |
| format | Article |
| id | doaj-art-b16353a57e4a443f949592a4b0cb9750 |
| institution | Kabale University |
| issn | 1751-7311 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| spelling | doaj-art-b16353a57e4a443f949592a4b0cb97502025-02-12T05:30:50ZengElsevierAnimal1751-73112025-02-01192101353Effect of 25-hydroxycholecalciferol and high phytase doses on performance, vitamin D status, bone mineralization, and mechanistic target of rapamycin gene expression of broilersL.S. Bassi0C.C.S. Martins1C.A. Lozano-Poveda2I.C. Dias3S.G. Oliveira4A. Maiorka5Department of Animal Science, Federal University of Paraná, Curitiba, PR 80035-050, Brazil; Corresponding author.dsm-firmenich, São Paulo, SP 03178-200, Brazildsm-firmenich, São Paulo, SP 03178-200, BrazilDepartment of Animal Science, Federal University of Paraná, Curitiba, PR 80035-050, BrazilDepartment of Animal Science, Federal University of Paraná, Curitiba, PR 80035-050, BrazilDepartment of Animal Science, Federal University of Paraná, Curitiba, PR 80035-050, BrazilThe use of exogenous phytase and vitamin D3 metabolites such as 25-hydroxycholecalciferol (25-OH-D3) for poultry is well consolidated, but the potential for additive effects when supplementing both requires further investigation. This study investigated possible interactions between supplementation of 25-OH-D3 and high doses of phytase for broilers fed Ca− and P-deficient diets. A total of 1 200 one-d-old male broiler chicks were randomly allocated from one of four dietary treatments in a 2 × 2 factorial arrangement: 600 or 2 000 phytase units (FYT)/kg and with or without the inclusion of 25-OH-D3 at 69 µg/kg, with 12 replicates of 25 broilers each. Two feeding phases were stablished (1-to-21 and 22-to-42 d) and all diets contained commercial levels of vitamin D3 (100 µg/kg) with total Ca and available P respectively set to 0.6 and 0.3%. Supplementation with 25-OH-D3 increased body weight gain (BWG) and reduced feed conversion ratio (FCR) from 1 to 21 d (P < 0.05), as well as increased BWG from 1 to 42 d (P < 0.05). Serum 25-OH-D3 levels at 21 and 42 d were increased with 25-OH-D3 (P < 0.001). Phytase did not affect growth performance from 1 to 21 d, but a higher dose (2 000 FYT/kg) reduced feed intake and FCR from 22 to 42 d compared to 600 FYT/kg, also reducing FCR in the total period (P < 0.05). The mRNA expression of mechanistic target of rapamycin in breast muscle assessed at 42 d was enhanced with 2 000 FYT/kg (P < 0.001). Bone weight, bone contents of ash, Ca, and P, and bone breaking strength of tibia bone measured at 42 d were not affected by any dietary treatment. Although both additives are known to improve dietary Ca and P utilization, there were no detected additive or synergic effects. The results suggested that the inclusion of 25-OH-D3 and phytase combined with regular vitamin D3 levels can help minimize losses of performance and bone mineralization of broilers fed Ca- and P-deficient diets. Furthermore, vitamin D status is refined with dietary 25-OH-D3 and potential improvements on breast meat yield can be obtained with a high phytase dose of 2 000 FYT/kg.http://www.sciencedirect.com/science/article/pii/S175173112400290825-hydroxyvitamin DBroiler chickensEnzymesMineralsVitamin D |
| spellingShingle | L.S. Bassi C.C.S. Martins C.A. Lozano-Poveda I.C. Dias S.G. Oliveira A. Maiorka Effect of 25-hydroxycholecalciferol and high phytase doses on performance, vitamin D status, bone mineralization, and mechanistic target of rapamycin gene expression of broilers Animal 25-hydroxyvitamin D Broiler chickens Enzymes Minerals Vitamin D |
| title | Effect of 25-hydroxycholecalciferol and high phytase doses on performance, vitamin D status, bone mineralization, and mechanistic target of rapamycin gene expression of broilers |
| title_full | Effect of 25-hydroxycholecalciferol and high phytase doses on performance, vitamin D status, bone mineralization, and mechanistic target of rapamycin gene expression of broilers |
| title_fullStr | Effect of 25-hydroxycholecalciferol and high phytase doses on performance, vitamin D status, bone mineralization, and mechanistic target of rapamycin gene expression of broilers |
| title_full_unstemmed | Effect of 25-hydroxycholecalciferol and high phytase doses on performance, vitamin D status, bone mineralization, and mechanistic target of rapamycin gene expression of broilers |
| title_short | Effect of 25-hydroxycholecalciferol and high phytase doses on performance, vitamin D status, bone mineralization, and mechanistic target of rapamycin gene expression of broilers |
| title_sort | effect of 25 hydroxycholecalciferol and high phytase doses on performance vitamin d status bone mineralization and mechanistic target of rapamycin gene expression of broilers |
| topic | 25-hydroxyvitamin D Broiler chickens Enzymes Minerals Vitamin D |
| url | http://www.sciencedirect.com/science/article/pii/S1751731124002908 |
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