Decoding B Cells in Autoimmune Diseases Through ScRNA + BCR-Seq: Current Knowledge and Future Directions
The combined application of single-cell RNA sequencing (scRNA-seq) and single-cell B-cell receptor sequencing (scBCR-seq) offers a multidimensional perspective for dissecting the immunopathological mechanisms of B cells in autoimmune diseases. This review systematically summarizes the principles of...
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MDPI AG
2025-04-01
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| author | Kai Quan Huifang Wang Peng Su Yuanyuan Xu Xinsheng Yao |
| author_facet | Kai Quan Huifang Wang Peng Su Yuanyuan Xu Xinsheng Yao |
| author_sort | Kai Quan |
| collection | DOAJ |
| description | The combined application of single-cell RNA sequencing (scRNA-seq) and single-cell B-cell receptor sequencing (scBCR-seq) offers a multidimensional perspective for dissecting the immunopathological mechanisms of B cells in autoimmune diseases. This review systematically summarizes the principles of these techniques, the analytical framework, and their key applications in diseases such as systemic lupus erythematosus et. al. It reveals the dynamic correlations between the transcriptome of B-cell subsets and B-cell receptor (BCR) clones. Furthermore, we focus on the potential roles of dual BCR B cells and B/T biphenotypic cells in autoimmunity, emphasizing their exacerbation of disease progression through abnormal clonal expansion and autoantibody secretion. By sorting through cutting-edge advancements and bottleneck issues, this article aims to propel the innovation of multi-omics research and precision treatment paradigms for autoimmune diseases. |
| format | Article |
| id | doaj-art-b15cfee8c7e249ab8f92ad4953a07db0 |
| institution | DOAJ |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj-art-b15cfee8c7e249ab8f92ad4953a07db02025-08-20T03:06:27ZengMDPI AGCells2073-44092025-04-0114753910.3390/cells14070539Decoding B Cells in Autoimmune Diseases Through ScRNA + BCR-Seq: Current Knowledge and Future DirectionsKai Quan0Huifang Wang1Peng Su2Yuanyuan Xu3Xinsheng Yao4Department of Immunology, Center of Immuno-Molecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563002, ChinaDepartment of Immunology, Center of Immuno-Molecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563002, ChinaDepartment of Immunology, Center of Immuno-Molecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563002, ChinaDepartment of Immunology, Center of Immuno-Molecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563002, ChinaDepartment of Immunology, Center of Immuno-Molecular Engineering, Innovation & Practice Base for Graduate Students Education, Zunyi Medical University, Zunyi 563002, ChinaThe combined application of single-cell RNA sequencing (scRNA-seq) and single-cell B-cell receptor sequencing (scBCR-seq) offers a multidimensional perspective for dissecting the immunopathological mechanisms of B cells in autoimmune diseases. This review systematically summarizes the principles of these techniques, the analytical framework, and their key applications in diseases such as systemic lupus erythematosus et. al. It reveals the dynamic correlations between the transcriptome of B-cell subsets and B-cell receptor (BCR) clones. Furthermore, we focus on the potential roles of dual BCR B cells and B/T biphenotypic cells in autoimmunity, emphasizing their exacerbation of disease progression through abnormal clonal expansion and autoantibody secretion. By sorting through cutting-edge advancements and bottleneck issues, this article aims to propel the innovation of multi-omics research and precision treatment paradigms for autoimmune diseases.https://www.mdpi.com/2073-4409/14/7/539single-cell RNA sequencingCDR3autoimmune diseasessingle-cell B-cell receptor sequencing |
| spellingShingle | Kai Quan Huifang Wang Peng Su Yuanyuan Xu Xinsheng Yao Decoding B Cells in Autoimmune Diseases Through ScRNA + BCR-Seq: Current Knowledge and Future Directions Cells single-cell RNA sequencing CDR3 autoimmune diseases single-cell B-cell receptor sequencing |
| title | Decoding B Cells in Autoimmune Diseases Through ScRNA + BCR-Seq: Current Knowledge and Future Directions |
| title_full | Decoding B Cells in Autoimmune Diseases Through ScRNA + BCR-Seq: Current Knowledge and Future Directions |
| title_fullStr | Decoding B Cells in Autoimmune Diseases Through ScRNA + BCR-Seq: Current Knowledge and Future Directions |
| title_full_unstemmed | Decoding B Cells in Autoimmune Diseases Through ScRNA + BCR-Seq: Current Knowledge and Future Directions |
| title_short | Decoding B Cells in Autoimmune Diseases Through ScRNA + BCR-Seq: Current Knowledge and Future Directions |
| title_sort | decoding b cells in autoimmune diseases through scrna bcr seq current knowledge and future directions |
| topic | single-cell RNA sequencing CDR3 autoimmune diseases single-cell B-cell receptor sequencing |
| url | https://www.mdpi.com/2073-4409/14/7/539 |
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