Characterization and Assembly Dynamics of the Microbiome Associated with Swine Anaerobic Lagoon Manure Treated with Biochar
Biochar has significant potential for livestock microbiomes and crop agriculture regarding greenhouse gas emissions reduction. Therefore, a pilot study was designed to investigate the effect of biochar application on the surface of swine manure from an open lagoon and the associated microbial commun...
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2025-03-01
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| author | A. Nathan Frazier William Willis Heather Robbe Anna Ortiz Jacek A. Koziel |
| author_facet | A. Nathan Frazier William Willis Heather Robbe Anna Ortiz Jacek A. Koziel |
| author_sort | A. Nathan Frazier |
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| description | Biochar has significant potential for livestock microbiomes and crop agriculture regarding greenhouse gas emissions reduction. Therefore, a pilot study was designed to investigate the effect of biochar application on the surface of swine manure from an open lagoon and the associated microbial communities. Samples were collected from four different treatment groups: control (<i>n</i> = 4), coarse biochar (<i>n</i> = 4), fine biochar (<i>n</i> = 4), and ultra-fine biochar (<i>n</i> = 4). Additionally, aged manure in bulk was collected (<i>n</i> = 4) to assess alterations from the control group. The method of 16S rRNA amplicon sequencing along with microbial analyses was performed. Diversity was significantly different between aged manure in bulk samples and all treatment groups (Kruskal–Wallis; <i>p</i> < 0.05). Additionally, distinct community compositions were seen using both weighted and unweighted UniFrac distance matrices (PERMANOVA; <i>p</i> < 0.01). Differential abundance analysis revealed four distinct features within all treatment groups that were enriched (q < 0.001): <i>Idiomarina</i> spp., <i>Geovibrio thiophilus</i>, <i>Parapusillimonas granuli</i>, and an uncultured Gammaproteobacteria species. Similarly, <i>Comamonas</i> spp. and <i>Brumimicrobium aurantiacum</i> (q-value < 0.001) were significantly depleted by all the treatments. Stochastic and functional analyses revealed that biochar treatments were not deterministically altering assembly patterns, and functional redundancy was evident regardless of compositional shifts. |
| format | Article |
| id | doaj-art-b15272efdc9a49ba9bbda98bcb1ba247 |
| institution | OA Journals |
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| language | English |
| publishDate | 2025-03-01 |
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| series | Microorganisms |
| spelling | doaj-art-b15272efdc9a49ba9bbda98bcb1ba2472025-08-20T02:18:20ZengMDPI AGMicroorganisms2076-26072025-03-0113475810.3390/microorganisms13040758Characterization and Assembly Dynamics of the Microbiome Associated with Swine Anaerobic Lagoon Manure Treated with BiocharA. Nathan Frazier0William Willis1Heather Robbe2Anna Ortiz3Jacek A. Koziel4United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Bushland, TX 79012, USAUnited States Department of Agriculture-Agricultural Research Service (USDA-ARS), Bushland, TX 79012, USAUnited States Department of Agriculture-Agricultural Research Service (USDA-ARS), Bushland, TX 79012, USAUnited States Department of Agriculture-Agricultural Research Service (USDA-ARS), Bushland, TX 79012, USAUnited States Department of Agriculture-Agricultural Research Service (USDA-ARS), Bushland, TX 79012, USABiochar has significant potential for livestock microbiomes and crop agriculture regarding greenhouse gas emissions reduction. Therefore, a pilot study was designed to investigate the effect of biochar application on the surface of swine manure from an open lagoon and the associated microbial communities. Samples were collected from four different treatment groups: control (<i>n</i> = 4), coarse biochar (<i>n</i> = 4), fine biochar (<i>n</i> = 4), and ultra-fine biochar (<i>n</i> = 4). Additionally, aged manure in bulk was collected (<i>n</i> = 4) to assess alterations from the control group. The method of 16S rRNA amplicon sequencing along with microbial analyses was performed. Diversity was significantly different between aged manure in bulk samples and all treatment groups (Kruskal–Wallis; <i>p</i> < 0.05). Additionally, distinct community compositions were seen using both weighted and unweighted UniFrac distance matrices (PERMANOVA; <i>p</i> < 0.01). Differential abundance analysis revealed four distinct features within all treatment groups that were enriched (q < 0.001): <i>Idiomarina</i> spp., <i>Geovibrio thiophilus</i>, <i>Parapusillimonas granuli</i>, and an uncultured Gammaproteobacteria species. Similarly, <i>Comamonas</i> spp. and <i>Brumimicrobium aurantiacum</i> (q-value < 0.001) were significantly depleted by all the treatments. Stochastic and functional analyses revealed that biochar treatments were not deterministically altering assembly patterns, and functional redundancy was evident regardless of compositional shifts.https://www.mdpi.com/2076-2607/13/4/758ammoniabiochargreenhouse gas emissionsmicrobial ecologyswine manure |
| spellingShingle | A. Nathan Frazier William Willis Heather Robbe Anna Ortiz Jacek A. Koziel Characterization and Assembly Dynamics of the Microbiome Associated with Swine Anaerobic Lagoon Manure Treated with Biochar Microorganisms ammonia biochar greenhouse gas emissions microbial ecology swine manure |
| title | Characterization and Assembly Dynamics of the Microbiome Associated with Swine Anaerobic Lagoon Manure Treated with Biochar |
| title_full | Characterization and Assembly Dynamics of the Microbiome Associated with Swine Anaerobic Lagoon Manure Treated with Biochar |
| title_fullStr | Characterization and Assembly Dynamics of the Microbiome Associated with Swine Anaerobic Lagoon Manure Treated with Biochar |
| title_full_unstemmed | Characterization and Assembly Dynamics of the Microbiome Associated with Swine Anaerobic Lagoon Manure Treated with Biochar |
| title_short | Characterization and Assembly Dynamics of the Microbiome Associated with Swine Anaerobic Lagoon Manure Treated with Biochar |
| title_sort | characterization and assembly dynamics of the microbiome associated with swine anaerobic lagoon manure treated with biochar |
| topic | ammonia biochar greenhouse gas emissions microbial ecology swine manure |
| url | https://www.mdpi.com/2076-2607/13/4/758 |
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