Mechanically regulated microcarriers with stem cell loading for skin photoaging therapy
Long-term exposure to ultraviolet radiation compromises skin structural integrity and results in disruption of normal physiological functions. Stem cells have gained attention in anti-photoaging, while controlling the tissue mechanical microenvironment of cell delivery sites is crucial for regulatin...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-04-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X24005619 |
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| author | Xiang Lin Anne M. Filppula Yuanjin Zhao Luoran Shang Hongbo Zhang |
| author_facet | Xiang Lin Anne M. Filppula Yuanjin Zhao Luoran Shang Hongbo Zhang |
| author_sort | Xiang Lin |
| collection | DOAJ |
| description | Long-term exposure to ultraviolet radiation compromises skin structural integrity and results in disruption of normal physiological functions. Stem cells have gained attention in anti-photoaging, while controlling the tissue mechanical microenvironment of cell delivery sites is crucial for regulating cell fate and achieving optimal therapeutic performances. Here, we introduce a mechanically regulated human recombinant collagen (RHC) microcarrier generated through microfluidics, which is capable of modulating stem cell differentiation to treat photoaged skin. By controlling the cross-linking parameters, the mechanical properties of microcarriers could precisely tuned to optimize the stem cell differentiation. The microcarriers are surface functionalized with fibronectin (Fn)-platelet derived growth factor-BB (PDGF-BB) to facilitate adipose derived mesenchymal stem cells (Ad-MSCs) loading. In in vivo experiments, subcutaneous injection of stem cell loaded RHC microcarriers significantly reduced skin wrinkles after ultraviolet-injury, effectively promoted collagen synthesis, and increased vascular density. These encouraging results indicate that the present mechanically regulated microcarriers have great potential to deliver stem cells and regulate their differentiation for anti-photoaging treatments. |
| format | Article |
| id | doaj-art-b14c3bfb735346fa9dc8f9068032e8e3 |
| institution | OA Journals |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-b14c3bfb735346fa9dc8f9068032e8e32025-08-20T02:15:24ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-04-014644845610.1016/j.bioactmat.2024.12.024Mechanically regulated microcarriers with stem cell loading for skin photoaging therapyXiang Lin0Anne M. Filppula1Yuanjin Zhao2Luoran Shang3Hongbo Zhang4Department of Gastrointestinal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325035, China; Joint Centre of Translational Medicine, Wenzhou Key Laboratory of Interdiscipline and Translational Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China; Pharmaceutical Sciences Laboratory, Åbo Akademi University, Turku, 20520, Finland; Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, ChinaPharmaceutical Sciences Laboratory, Åbo Akademi University, Turku, 20520, FinlandDepartment of Gastrointestinal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325035, China; Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China; Corresponding author. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325035, China.Shanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, and the Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology, Institutes of Biomedical Sciences), Fudan University, Shanghai, 200032, China; Corresponding author.Department of Gastrointestinal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325035, China; Joint Centre of Translational Medicine, Wenzhou Key Laboratory of Interdiscipline and Translational Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China; Pharmaceutical Sciences Laboratory, Åbo Akademi University, Turku, 20520, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, 20520, Finland; Corresponding author. Department of Gastrointestinal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325035, China.Long-term exposure to ultraviolet radiation compromises skin structural integrity and results in disruption of normal physiological functions. Stem cells have gained attention in anti-photoaging, while controlling the tissue mechanical microenvironment of cell delivery sites is crucial for regulating cell fate and achieving optimal therapeutic performances. Here, we introduce a mechanically regulated human recombinant collagen (RHC) microcarrier generated through microfluidics, which is capable of modulating stem cell differentiation to treat photoaged skin. By controlling the cross-linking parameters, the mechanical properties of microcarriers could precisely tuned to optimize the stem cell differentiation. The microcarriers are surface functionalized with fibronectin (Fn)-platelet derived growth factor-BB (PDGF-BB) to facilitate adipose derived mesenchymal stem cells (Ad-MSCs) loading. In in vivo experiments, subcutaneous injection of stem cell loaded RHC microcarriers significantly reduced skin wrinkles after ultraviolet-injury, effectively promoted collagen synthesis, and increased vascular density. These encouraging results indicate that the present mechanically regulated microcarriers have great potential to deliver stem cells and regulate their differentiation for anti-photoaging treatments.http://www.sciencedirect.com/science/article/pii/S2452199X24005619MicrocarriersMechanical regulationHydrogelStem cellPhotoaging therapy |
| spellingShingle | Xiang Lin Anne M. Filppula Yuanjin Zhao Luoran Shang Hongbo Zhang Mechanically regulated microcarriers with stem cell loading for skin photoaging therapy Bioactive Materials Microcarriers Mechanical regulation Hydrogel Stem cell Photoaging therapy |
| title | Mechanically regulated microcarriers with stem cell loading for skin photoaging therapy |
| title_full | Mechanically regulated microcarriers with stem cell loading for skin photoaging therapy |
| title_fullStr | Mechanically regulated microcarriers with stem cell loading for skin photoaging therapy |
| title_full_unstemmed | Mechanically regulated microcarriers with stem cell loading for skin photoaging therapy |
| title_short | Mechanically regulated microcarriers with stem cell loading for skin photoaging therapy |
| title_sort | mechanically regulated microcarriers with stem cell loading for skin photoaging therapy |
| topic | Microcarriers Mechanical regulation Hydrogel Stem cell Photoaging therapy |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X24005619 |
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