Lysosomal Exoglycosidase Profile and Secretory Function in the Salivary Glands of Rats with Streptozotocin-Induced Diabetes
Before this study, there had been no research evaluating the relationship between a lysosomal exoglycosidase profile and secretory function in the salivary glands of rats with streptozotocin- (STZ-) induced type 1 diabetes. In our work, rats were divided into 4 groups of 8 animals each: control grou...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/9850398 |
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| author | Mateusz Maciejczyk Agnieszka Kossakowska Julita Szulimowska Anna Klimiuk Małgorzata Knaś Halina Car Wiesława Niklińska Jerzy Robert Ładny Adrian Chabowski Anna Zalewska |
| author_facet | Mateusz Maciejczyk Agnieszka Kossakowska Julita Szulimowska Anna Klimiuk Małgorzata Knaś Halina Car Wiesława Niklińska Jerzy Robert Ładny Adrian Chabowski Anna Zalewska |
| author_sort | Mateusz Maciejczyk |
| collection | DOAJ |
| description | Before this study, there had been no research evaluating the relationship between a lysosomal exoglycosidase profile and secretory function in the salivary glands of rats with streptozotocin- (STZ-) induced type 1 diabetes. In our work, rats were divided into 4 groups of 8 animals each: control groups (C2, C4) and diabetic groups (STZ2, STZ4). The secretory function of salivary glands—nonstimulated and stimulated salivary flow, α-amylase, total protein—and salivary exoglycosidase activities—N-acetyl-β-hexosaminidase (HEX, HEX A, and HEX B), β-glucuronidase, α-fucosidase, β-galactosidase, and α-mannosidase—was estimated both in the parotid and submandibular glands of STZ-diabetic and control rats. The study has demonstrated that the activity of most salivary exoglycosidases is significantly higher in the parotid and submandibular glands of STZ-diabetic rats as compared to the healthy controls and that it increases as the disease progresses. Reduced secretory function of diabetic salivary glands was also observed. A significant inverse correlation between HEX B, α-amylase activity, and stimulated salivary flow in diabetic parotid gland has also been shown. Summarizing, STZ-induced diabetes leads to a change in the lysosomal exoglycosidase profile and reduced function of the salivary glands. |
| format | Article |
| id | doaj-art-b136fc006c8c4243a442935692231d34 |
| institution | DOAJ |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-b136fc006c8c4243a442935692231d342025-08-20T03:20:47ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/98503989850398Lysosomal Exoglycosidase Profile and Secretory Function in the Salivary Glands of Rats with Streptozotocin-Induced DiabetesMateusz Maciejczyk0Agnieszka Kossakowska1Julita Szulimowska2Anna Klimiuk3Małgorzata Knaś4Halina Car5Wiesława Niklińska6Jerzy Robert Ładny7Adrian Chabowski8Anna Zalewska9Department of Physiology, Medical University of Bialystok, 2c Mickiewicza Street, 15-233 Bialystok, PolandDepartment of Conservative Dentistry, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, PolandDepartment of Pedodontics, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, PolandDepartment of Conservative Dentistry, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, PolandDepartment of Cosmetology, Lomza State University of Applied Sciences, Akademicka 1 str, 18-400 Lomza, PolandDepartment of Experimental Pharmacology, Medical University of Bialystok, 37 Szpitalna Street, 15-767 Bialystok, PolandDepartment of Histology and Embryology, Medical University of Bialystok, 13 Waszyngtona Street, Bialystok, PolandDepartment of Emergency Medicine and Disasters, Medical University of Bialystok, 37 Szpitalna Street, 15-767 Bialystok, PolandDepartment of Physiology, Medical University of Bialystok, 2c Mickiewicza Street, 15-233 Bialystok, PolandDepartment of Conservative Dentistry, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, PolandBefore this study, there had been no research evaluating the relationship between a lysosomal exoglycosidase profile and secretory function in the salivary glands of rats with streptozotocin- (STZ-) induced type 1 diabetes. In our work, rats were divided into 4 groups of 8 animals each: control groups (C2, C4) and diabetic groups (STZ2, STZ4). The secretory function of salivary glands—nonstimulated and stimulated salivary flow, α-amylase, total protein—and salivary exoglycosidase activities—N-acetyl-β-hexosaminidase (HEX, HEX A, and HEX B), β-glucuronidase, α-fucosidase, β-galactosidase, and α-mannosidase—was estimated both in the parotid and submandibular glands of STZ-diabetic and control rats. The study has demonstrated that the activity of most salivary exoglycosidases is significantly higher in the parotid and submandibular glands of STZ-diabetic rats as compared to the healthy controls and that it increases as the disease progresses. Reduced secretory function of diabetic salivary glands was also observed. A significant inverse correlation between HEX B, α-amylase activity, and stimulated salivary flow in diabetic parotid gland has also been shown. Summarizing, STZ-induced diabetes leads to a change in the lysosomal exoglycosidase profile and reduced function of the salivary glands.http://dx.doi.org/10.1155/2017/9850398 |
| spellingShingle | Mateusz Maciejczyk Agnieszka Kossakowska Julita Szulimowska Anna Klimiuk Małgorzata Knaś Halina Car Wiesława Niklińska Jerzy Robert Ładny Adrian Chabowski Anna Zalewska Lysosomal Exoglycosidase Profile and Secretory Function in the Salivary Glands of Rats with Streptozotocin-Induced Diabetes Journal of Diabetes Research |
| title | Lysosomal Exoglycosidase Profile and Secretory Function in the Salivary Glands of Rats with Streptozotocin-Induced Diabetes |
| title_full | Lysosomal Exoglycosidase Profile and Secretory Function in the Salivary Glands of Rats with Streptozotocin-Induced Diabetes |
| title_fullStr | Lysosomal Exoglycosidase Profile and Secretory Function in the Salivary Glands of Rats with Streptozotocin-Induced Diabetes |
| title_full_unstemmed | Lysosomal Exoglycosidase Profile and Secretory Function in the Salivary Glands of Rats with Streptozotocin-Induced Diabetes |
| title_short | Lysosomal Exoglycosidase Profile and Secretory Function in the Salivary Glands of Rats with Streptozotocin-Induced Diabetes |
| title_sort | lysosomal exoglycosidase profile and secretory function in the salivary glands of rats with streptozotocin induced diabetes |
| url | http://dx.doi.org/10.1155/2017/9850398 |
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