Novel Pathogenicity of Secretory Proteins from Acinetobacter Radioresistens in Experimental Models
Background: Acinetobacter species are environmental microorganisms that inhabit the skin and can become opportunistic pathogens in immunocompromised hosts. This study investigated the pathogenicity of proteins released extracellularly by Acinetobacter radioresistens (A. radioresistens), isolated fro...
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| Format: | Article |
| Language: | English |
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Society of Tokyo Women's Medical University
2024-03-01
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| Series: | Tokyo Women's Medical University Journal |
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| Online Access: | https://www.jstage.jst.go.jp/article/twmuj/8/0/8_2023019/_pdf/-char/en |
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| author | Yuki Suzuki Toshifumi Osaka Hidehiro Ueshiba Naoko Yanagisawa Satoru Nagata |
| author_facet | Yuki Suzuki Toshifumi Osaka Hidehiro Ueshiba Naoko Yanagisawa Satoru Nagata |
| author_sort | Yuki Suzuki |
| collection | DOAJ |
| description | Background: Acinetobacter species are environmental microorganisms that inhabit the skin and can become opportunistic pathogens in immunocompromised hosts. This study investigated the pathogenicity of proteins released extracellularly by Acinetobacter radioresistens (A. radioresistens), isolated from the small intestine of two patients with severe Kawasaki disease, in a mouse model. Methods: The sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of culture supernatants derived from Acinetobacter species showed large variations in protein compositions among species and strains. The biological effects of soluble proteins secreted by A. radioresistens were evaluated in mice by intraperitoneal injection of culture supernatants. Results: The administration of culture supernatant derived from A. radioresistens caused weight loss, electrolyte imbalance, and decreased aldosterone levels. The sustained infiltration of the inflammatory mononuclear cells was observed in the liver of mice 6 weeks after the last administration. Conclusions: A. radioresistens expressed virulence through its extracellular proteins, leading to acute adrenal gland insufficiency and chronic inflammation of the livers. |
| format | Article |
| id | doaj-art-b12ea0c7997f417ab189c49494f1c113 |
| institution | DOAJ |
| issn | 2432-6186 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Society of Tokyo Women's Medical University |
| record_format | Article |
| series | Tokyo Women's Medical University Journal |
| spelling | doaj-art-b12ea0c7997f417ab189c49494f1c1132025-08-20T02:39:31ZengSociety of Tokyo Women's Medical UniversityTokyo Women's Medical University Journal2432-61862024-03-0180243710.24488/twmuj.2023019twmujNovel Pathogenicity of Secretory Proteins from Acinetobacter Radioresistens in Experimental ModelsYuki Suzuki0Toshifumi Osaka1Hidehiro Ueshiba2Naoko Yanagisawa3Satoru Nagata4Department of Pediatrics, Tokyo Women's Medical UniversityDepartment of Microbiology and Immunology, Tokyo Women's Medical UniversityDepartment of Microbiology and Immunology, Tokyo Women's Medical UniversityDepartment of Microbiology and Immunology, Tokyo Women's Medical UniversityDepartment of Pediatrics, Tokyo Women's Medical UniversityBackground: Acinetobacter species are environmental microorganisms that inhabit the skin and can become opportunistic pathogens in immunocompromised hosts. This study investigated the pathogenicity of proteins released extracellularly by Acinetobacter radioresistens (A. radioresistens), isolated from the small intestine of two patients with severe Kawasaki disease, in a mouse model. Methods: The sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of culture supernatants derived from Acinetobacter species showed large variations in protein compositions among species and strains. The biological effects of soluble proteins secreted by A. radioresistens were evaluated in mice by intraperitoneal injection of culture supernatants. Results: The administration of culture supernatant derived from A. radioresistens caused weight loss, electrolyte imbalance, and decreased aldosterone levels. The sustained infiltration of the inflammatory mononuclear cells was observed in the liver of mice 6 weeks after the last administration. Conclusions: A. radioresistens expressed virulence through its extracellular proteins, leading to acute adrenal gland insufficiency and chronic inflammation of the livers.https://www.jstage.jst.go.jp/article/twmuj/8/0/8_2023019/_pdf/-char/enacinetobacter radioresistenssecretory proteinshepatitisaldosteroneelectrolyte imbalance |
| spellingShingle | Yuki Suzuki Toshifumi Osaka Hidehiro Ueshiba Naoko Yanagisawa Satoru Nagata Novel Pathogenicity of Secretory Proteins from Acinetobacter Radioresistens in Experimental Models Tokyo Women's Medical University Journal acinetobacter radioresistens secretory proteins hepatitis aldosterone electrolyte imbalance |
| title | Novel Pathogenicity of Secretory Proteins from Acinetobacter Radioresistens in Experimental Models |
| title_full | Novel Pathogenicity of Secretory Proteins from Acinetobacter Radioresistens in Experimental Models |
| title_fullStr | Novel Pathogenicity of Secretory Proteins from Acinetobacter Radioresistens in Experimental Models |
| title_full_unstemmed | Novel Pathogenicity of Secretory Proteins from Acinetobacter Radioresistens in Experimental Models |
| title_short | Novel Pathogenicity of Secretory Proteins from Acinetobacter Radioresistens in Experimental Models |
| title_sort | novel pathogenicity of secretory proteins from acinetobacter radioresistens in experimental models |
| topic | acinetobacter radioresistens secretory proteins hepatitis aldosterone electrolyte imbalance |
| url | https://www.jstage.jst.go.jp/article/twmuj/8/0/8_2023019/_pdf/-char/en |
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