Agromyces arachidis sp. nov. Isolated from a Peanut (Arachis hypogaea) Crop Field

A Gram-positive, yellowish bacterium strain AK-1T was isolated from soil sample collected from peanut (Arachis hypogaea) crop field and studied by using a polyphasic approach. The organism had morphological and chemotaxonomic properties consistent with its classification in the genus Agromyces. Phyl...

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Main Authors: Chandandeep Kaur, Anil Kumar Pinnaka, Nitin Kumar Singh, Monu Bala, Shanmugam Mayilraj
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2013/831308
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author Chandandeep Kaur
Anil Kumar Pinnaka
Nitin Kumar Singh
Monu Bala
Shanmugam Mayilraj
author_facet Chandandeep Kaur
Anil Kumar Pinnaka
Nitin Kumar Singh
Monu Bala
Shanmugam Mayilraj
author_sort Chandandeep Kaur
collection DOAJ
description A Gram-positive, yellowish bacterium strain AK-1T was isolated from soil sample collected from peanut (Arachis hypogaea) crop field and studied by using a polyphasic approach. The organism had morphological and chemotaxonomic properties consistent with its classification in the genus Agromyces. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain AK-1T was closely related to Agromyces aurantiacus (98.6%) followed by Agromyces soli (98.3%), Agromyces tropicus (97.6%), Agromyces ulmi (97.3%), Agromyces flavus (97.2%), and Agromyces italicus (97.0%), whereas the sequence similarity values with respect to the other Agromyces species with validly published names were between 95.3 and 96.7 %. However, the DNA-DNA hybridization values obtained between strain AK-1T and other related strains were well below the threshold that is required for the proposal of a novel species. The DNA G+C content of the strain is 71.8 mol%. The above data in combination with the phenotypic distinctiveness of AK-1T clearly indicate that the strain represents a novel species, for which the name Agromyces arachidis sp. nov. is proposed. The type strain is AK-1T (=MTCC 10524T = JCM 19251T).
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series International Journal of Microbiology
spelling doaj-art-b11fca4786f4467c828a4a8017d65b0f2025-08-20T02:23:15ZengWileyInternational Journal of Microbiology1687-918X1687-91982013-01-01201310.1155/2013/831308831308Agromyces arachidis sp. nov. Isolated from a Peanut (Arachis hypogaea) Crop FieldChandandeep Kaur0Anil Kumar Pinnaka1Nitin Kumar Singh2Monu Bala3Shanmugam Mayilraj4Microbial Type Culture Collection and Gene Bank (MTCC), CSIR Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160 036, IndiaMicrobial Type Culture Collection and Gene Bank (MTCC), CSIR Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160 036, IndiaMicrobial Type Culture Collection and Gene Bank (MTCC), CSIR Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160 036, IndiaMicrobial Type Culture Collection and Gene Bank (MTCC), CSIR Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160 036, IndiaMicrobial Type Culture Collection and Gene Bank (MTCC), CSIR Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh 160 036, IndiaA Gram-positive, yellowish bacterium strain AK-1T was isolated from soil sample collected from peanut (Arachis hypogaea) crop field and studied by using a polyphasic approach. The organism had morphological and chemotaxonomic properties consistent with its classification in the genus Agromyces. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain AK-1T was closely related to Agromyces aurantiacus (98.6%) followed by Agromyces soli (98.3%), Agromyces tropicus (97.6%), Agromyces ulmi (97.3%), Agromyces flavus (97.2%), and Agromyces italicus (97.0%), whereas the sequence similarity values with respect to the other Agromyces species with validly published names were between 95.3 and 96.7 %. However, the DNA-DNA hybridization values obtained between strain AK-1T and other related strains were well below the threshold that is required for the proposal of a novel species. The DNA G+C content of the strain is 71.8 mol%. The above data in combination with the phenotypic distinctiveness of AK-1T clearly indicate that the strain represents a novel species, for which the name Agromyces arachidis sp. nov. is proposed. The type strain is AK-1T (=MTCC 10524T = JCM 19251T).http://dx.doi.org/10.1155/2013/831308
spellingShingle Chandandeep Kaur
Anil Kumar Pinnaka
Nitin Kumar Singh
Monu Bala
Shanmugam Mayilraj
Agromyces arachidis sp. nov. Isolated from a Peanut (Arachis hypogaea) Crop Field
International Journal of Microbiology
title Agromyces arachidis sp. nov. Isolated from a Peanut (Arachis hypogaea) Crop Field
title_full Agromyces arachidis sp. nov. Isolated from a Peanut (Arachis hypogaea) Crop Field
title_fullStr Agromyces arachidis sp. nov. Isolated from a Peanut (Arachis hypogaea) Crop Field
title_full_unstemmed Agromyces arachidis sp. nov. Isolated from a Peanut (Arachis hypogaea) Crop Field
title_short Agromyces arachidis sp. nov. Isolated from a Peanut (Arachis hypogaea) Crop Field
title_sort agromyces arachidis sp nov isolated from a peanut arachis hypogaea crop field
url http://dx.doi.org/10.1155/2013/831308
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