Cascade-heterogated proton nanotransistors for multiplex pH-interval imaging
Abstract The pH in organisms is strictly regulated within an appropriate range for homeostasis, and many pathological processes often result in pH dysregulation to distinct intervals that serves as targets for diagnosis and treatment. Thus, a mechanism of selective and gating response for different...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-61255-6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849402010923696128 |
|---|---|
| author | Ye Yang Binlong Chen Fangjie Wan Chuanxun Fu Meifang Chen Bin Ma Ruiyang Zhao Meijie Pan Qiang Zhang Yiguang Wang |
| author_facet | Ye Yang Binlong Chen Fangjie Wan Chuanxun Fu Meifang Chen Bin Ma Ruiyang Zhao Meijie Pan Qiang Zhang Yiguang Wang |
| author_sort | Ye Yang |
| collection | DOAJ |
| description | Abstract The pH in organisms is strictly regulated within an appropriate range for homeostasis, and many pathological processes often result in pH dysregulation to distinct intervals that serves as targets for diagnosis and treatment. Thus, a mechanism of selective and gating response for different proton levels in an integrated nanodevice is needed to report acidic biological environments with specific pH dynamic ranges. Here we report the design of band-pass light-up nanokits (BLINK) with pH heterogating capacity in opposite directions to achieve selective reporting of the acidic intervals (minimum to 0.25 pH unit) of pathophysiological environments. The proton heterogated property of BLINK integrates ultra-fast (<15 ms) and cascade nanophase transition of transistor-like polymeric modules and tandem FRET relay between encoded fluorophores, permitting the selective transformation of acidic extracellular or intracellular signals to exponentially amplified fluorescence readout without introducing the signals outside the targeted pH ranges. This study offers a valuable tool for pH sensing, and paves the way for the development of interval-based optical imaging technology. |
| format | Article |
| id | doaj-art-b113220593bf43cb91e322f94ea0e3d5 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-b113220593bf43cb91e322f94ea0e3d52025-08-20T03:37:38ZengNature PortfolioNature Communications2041-17232025-07-0116111310.1038/s41467-025-61255-6Cascade-heterogated proton nanotransistors for multiplex pH-interval imagingYe Yang0Binlong Chen1Fangjie Wan2Chuanxun Fu3Meifang Chen4Bin Ma5Ruiyang Zhao6Meijie Pan7Qiang Zhang8Yiguang Wang9State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking UniversityAbstract The pH in organisms is strictly regulated within an appropriate range for homeostasis, and many pathological processes often result in pH dysregulation to distinct intervals that serves as targets for diagnosis and treatment. Thus, a mechanism of selective and gating response for different proton levels in an integrated nanodevice is needed to report acidic biological environments with specific pH dynamic ranges. Here we report the design of band-pass light-up nanokits (BLINK) with pH heterogating capacity in opposite directions to achieve selective reporting of the acidic intervals (minimum to 0.25 pH unit) of pathophysiological environments. The proton heterogated property of BLINK integrates ultra-fast (<15 ms) and cascade nanophase transition of transistor-like polymeric modules and tandem FRET relay between encoded fluorophores, permitting the selective transformation of acidic extracellular or intracellular signals to exponentially amplified fluorescence readout without introducing the signals outside the targeted pH ranges. This study offers a valuable tool for pH sensing, and paves the way for the development of interval-based optical imaging technology.https://doi.org/10.1038/s41467-025-61255-6 |
| spellingShingle | Ye Yang Binlong Chen Fangjie Wan Chuanxun Fu Meifang Chen Bin Ma Ruiyang Zhao Meijie Pan Qiang Zhang Yiguang Wang Cascade-heterogated proton nanotransistors for multiplex pH-interval imaging Nature Communications |
| title | Cascade-heterogated proton nanotransistors for multiplex pH-interval imaging |
| title_full | Cascade-heterogated proton nanotransistors for multiplex pH-interval imaging |
| title_fullStr | Cascade-heterogated proton nanotransistors for multiplex pH-interval imaging |
| title_full_unstemmed | Cascade-heterogated proton nanotransistors for multiplex pH-interval imaging |
| title_short | Cascade-heterogated proton nanotransistors for multiplex pH-interval imaging |
| title_sort | cascade heterogated proton nanotransistors for multiplex ph interval imaging |
| url | https://doi.org/10.1038/s41467-025-61255-6 |
| work_keys_str_mv | AT yeyang cascadeheterogatedprotonnanotransistorsformultiplexphintervalimaging AT binlongchen cascadeheterogatedprotonnanotransistorsformultiplexphintervalimaging AT fangjiewan cascadeheterogatedprotonnanotransistorsformultiplexphintervalimaging AT chuanxunfu cascadeheterogatedprotonnanotransistorsformultiplexphintervalimaging AT meifangchen cascadeheterogatedprotonnanotransistorsformultiplexphintervalimaging AT binma cascadeheterogatedprotonnanotransistorsformultiplexphintervalimaging AT ruiyangzhao cascadeheterogatedprotonnanotransistorsformultiplexphintervalimaging AT meijiepan cascadeheterogatedprotonnanotransistorsformultiplexphintervalimaging AT qiangzhang cascadeheterogatedprotonnanotransistorsformultiplexphintervalimaging AT yiguangwang cascadeheterogatedprotonnanotransistorsformultiplexphintervalimaging |