Uncoupling protein gene UCP1-3826A/G, UCP2 Ins/Del and UCP3-55C/T polymorphisms in obese Turkish children
Background. Mitochondrial uncoupling proteins (UCP) 1, 2 and 3 are members of the anion carrier protein family located in the inner mitochondrial membrane. There are various controversial reports on UCP genotypes and obesity in adults and children. This study aims to investigate the link betw...
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| Format: | Article |
| Language: | English |
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Hacettepe University Institute of Child Health
2020-12-01
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| Series: | The Turkish Journal of Pediatrics |
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| Online Access: | https://turkjpediatr.org/article/view/534 |
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| author | Hasibe Verdi Sibel Tulgar Kınık H Pınar Baysan-Çebi Yaprak Yılmaz Yalçın Ayşe Canan Yazıcı-Güvercin Beril Aydın Neslihan Başcıl Tütüncü F Belgin Ataç |
| author_facet | Hasibe Verdi Sibel Tulgar Kınık H Pınar Baysan-Çebi Yaprak Yılmaz Yalçın Ayşe Canan Yazıcı-Güvercin Beril Aydın Neslihan Başcıl Tütüncü F Belgin Ataç |
| author_sort | Hasibe Verdi |
| collection | DOAJ |
| description |
Background. Mitochondrial uncoupling proteins (UCP) 1, 2 and 3 are members of the anion carrier protein family located in the inner mitochondrial membrane. There are various controversial reports on UCP genotypes and obesity in adults and children. This study aims to investigate the link between mostly studied UCP polymorphisms (UCP1-3826A/G, UCP2 Insertion/Deletion (Ins/Del) polymorphism of exon 8, and UCP3-55C/T Polymorphisms) and obesity in Turkish children. Furthermore, the relationships of UCP polymorphisms are also analyzed within the scope of metabolic parameters of obese children.
Methods. Molecular screening of the UCP1, UCP2, and UCP3 gene polymorphisms was carried out in 189 children aged 6 to 18 years, 102 of who had exogenous obesity (54 girls) and 87 of whom were healthy controls (48 girls). In the obese group, fasting lipids, glucose and insulin levels were measured. In 60 obese children, an oral glucose tolerance test (OGTT) was performed with 0, 30, 60, 90 and 120 minutes of sampling for plasma glucose and insulin levels.
Results. The frequency of UCP polymorphisms was similar in obese and non-obese children. In obese children, fasting lipids, glucose and insulin levels were not different among the UCP 1, 2 and 3 genotypes. While no relationship was found between the UCP 1 and 3 genotypes and glucose/insulin levels during OGTT, carriers of the Insertion allele with UCP2 Ins/Del polymorphism had significantly higher 30-minute insulin levels (p=0.018).
Conclusions. Polymorphisms of the UCP1-3826A/G, UCP2 Ins/Del, and UCP3-55C/T are not associated with obesity and related pathologies in Turkish children. However, the presence of the Ins allele of the UCP2 gene has been found to have an unfavorable influence on early insulin excursion after glucose loading.
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| format | Article |
| id | doaj-art-b1114fa8cd3c4324b39ecda2ff77d4bb |
| institution | OA Journals |
| issn | 0041-4301 2791-6421 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Hacettepe University Institute of Child Health |
| record_format | Article |
| series | The Turkish Journal of Pediatrics |
| spelling | doaj-art-b1114fa8cd3c4324b39ecda2ff77d4bb2025-08-20T02:01:51ZengHacettepe University Institute of Child HealthThe Turkish Journal of Pediatrics0041-43012791-64212020-12-0162610.24953/turkjped.2020.06.003Uncoupling protein gene UCP1-3826A/G, UCP2 Ins/Del and UCP3-55C/T polymorphisms in obese Turkish childrenHasibe Verdi0Sibel Tulgar Kınık1H Pınar Baysan-Çebi2Yaprak Yılmaz Yalçın3Ayşe Canan Yazıcı-Güvercin4Beril Aydın5Neslihan Başcıl Tütüncü6F Belgin Ataç7Departments of Medical Biology, Başkent University Faculty of Medicine, Ankara.Departments of Medical Biology, Başkent University Faculty of Medicine, Ankara.Departments of Medical Biology, Başkent University Faculty of Medicine, Ankara.Departments of Medical Biology, Başkent University Faculty of Medicine, Ankara.Department of Biostatistics, İstanbul Aydın University, Faculty of Medicine, İstanbul, Turkey.Departments of Pediatrics, Başkent University Faculty of Medicine, Ankara.Departments of Endocrinology and Metabolism, Başkent University Faculty of Medicine, Ankara.Departments of Medical Biology, Başkent University Faculty of Medicine, Ankara. Background. Mitochondrial uncoupling proteins (UCP) 1, 2 and 3 are members of the anion carrier protein family located in the inner mitochondrial membrane. There are various controversial reports on UCP genotypes and obesity in adults and children. This study aims to investigate the link between mostly studied UCP polymorphisms (UCP1-3826A/G, UCP2 Insertion/Deletion (Ins/Del) polymorphism of exon 8, and UCP3-55C/T Polymorphisms) and obesity in Turkish children. Furthermore, the relationships of UCP polymorphisms are also analyzed within the scope of metabolic parameters of obese children. Methods. Molecular screening of the UCP1, UCP2, and UCP3 gene polymorphisms was carried out in 189 children aged 6 to 18 years, 102 of who had exogenous obesity (54 girls) and 87 of whom were healthy controls (48 girls). In the obese group, fasting lipids, glucose and insulin levels were measured. In 60 obese children, an oral glucose tolerance test (OGTT) was performed with 0, 30, 60, 90 and 120 minutes of sampling for plasma glucose and insulin levels. Results. The frequency of UCP polymorphisms was similar in obese and non-obese children. In obese children, fasting lipids, glucose and insulin levels were not different among the UCP 1, 2 and 3 genotypes. While no relationship was found between the UCP 1 and 3 genotypes and glucose/insulin levels during OGTT, carriers of the Insertion allele with UCP2 Ins/Del polymorphism had significantly higher 30-minute insulin levels (p=0.018). Conclusions. Polymorphisms of the UCP1-3826A/G, UCP2 Ins/Del, and UCP3-55C/T are not associated with obesity and related pathologies in Turkish children. However, the presence of the Ins allele of the UCP2 gene has been found to have an unfavorable influence on early insulin excursion after glucose loading. https://turkjpediatr.org/article/view/534OGTTUCPchildrenobesepolymorphism |
| spellingShingle | Hasibe Verdi Sibel Tulgar Kınık H Pınar Baysan-Çebi Yaprak Yılmaz Yalçın Ayşe Canan Yazıcı-Güvercin Beril Aydın Neslihan Başcıl Tütüncü F Belgin Ataç Uncoupling protein gene UCP1-3826A/G, UCP2 Ins/Del and UCP3-55C/T polymorphisms in obese Turkish children The Turkish Journal of Pediatrics OGTT UCP children obese polymorphism |
| title | Uncoupling protein gene UCP1-3826A/G, UCP2 Ins/Del and UCP3-55C/T polymorphisms in obese Turkish children |
| title_full | Uncoupling protein gene UCP1-3826A/G, UCP2 Ins/Del and UCP3-55C/T polymorphisms in obese Turkish children |
| title_fullStr | Uncoupling protein gene UCP1-3826A/G, UCP2 Ins/Del and UCP3-55C/T polymorphisms in obese Turkish children |
| title_full_unstemmed | Uncoupling protein gene UCP1-3826A/G, UCP2 Ins/Del and UCP3-55C/T polymorphisms in obese Turkish children |
| title_short | Uncoupling protein gene UCP1-3826A/G, UCP2 Ins/Del and UCP3-55C/T polymorphisms in obese Turkish children |
| title_sort | uncoupling protein gene ucp1 3826a g ucp2 ins del and ucp3 55c t polymorphisms in obese turkish children |
| topic | OGTT UCP children obese polymorphism |
| url | https://turkjpediatr.org/article/view/534 |
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