Toxoplasmosis in Sheep Caused by <i>Toxoplasma gondii</i> Clonal Type I
Ovine toxoplasmosis has a significant negative impact on sheep farming, and abortion is the main clinical manifestation. The objective of the present study was to survey ovine toxoplasmosis and assess its potential harm <i>T. gondii</i> in sheep. Sheep serum or heart fluid was collected...
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MDPI AG
2025-04-01
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| author | Yurong Yang Yiheng Ma Kai Quan Bingyan Guo Yibao Jiang |
| author_facet | Yurong Yang Yiheng Ma Kai Quan Bingyan Guo Yibao Jiang |
| author_sort | Yurong Yang |
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| description | Ovine toxoplasmosis has a significant negative impact on sheep farming, and abortion is the main clinical manifestation. The objective of the present study was to survey ovine toxoplasmosis and assess its potential harm <i>T. gondii</i> in sheep. Sheep serum or heart fluid was collected from 1035 sheep, along with the tissue or blood samples collected from 164 of them. We investigated ovine toxoplasmosis by a modified agglutination test (MAT) (<i>n</i> = 1035 sheep) and by a polymerase chain reaction (PCR) (<i>n</i> = 164 sheep) in China. The results showed that 75 sheep were seropositive (titer ≥ 1:100), with a prevalence of 7.2%, as assessed by MAT. The molecular prevalence of <i>T. gondii</i> in sheep was 16.4% (27/164), as assessed by PCR. From these 164 tissue or blood samples, 22 tissues and 2 blood samples were selected in order to isolate <i>T. gondii</i> by mouse bioassay. A viable <i>T. gondii</i> strain (TgSheepCHn15, ToxoDB #10) was isolated from sheep tissues in the veterinary clinic. This strain was avirulent for Swiss mice, and the survival time of mice was 97 ± 31 days. This is the first instance of the isolation of a Type I strain from sheep with avirulence in mice. The prevalence of <i>T. gondii</i> has been decreasing in sheep from China. However, <i>T. gondii</i> remains present in sheep herds and should not be ignored. |
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| spelling | doaj-art-b10dc0c8c1e54576b463b9458b3b08452025-08-20T02:17:19ZengMDPI AGAnimals2076-26152025-04-01158107410.3390/ani15081074Toxoplasmosis in Sheep Caused by <i>Toxoplasma gondii</i> Clonal Type IYurong Yang0Yiheng Ma1Kai Quan2Bingyan Guo3Yibao Jiang4College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, ChinaCollege of Animal Science, Henan University of Animal Husbandry and Economy, Zhengzhou 450000, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450000, ChinaOvine toxoplasmosis has a significant negative impact on sheep farming, and abortion is the main clinical manifestation. The objective of the present study was to survey ovine toxoplasmosis and assess its potential harm <i>T. gondii</i> in sheep. Sheep serum or heart fluid was collected from 1035 sheep, along with the tissue or blood samples collected from 164 of them. We investigated ovine toxoplasmosis by a modified agglutination test (MAT) (<i>n</i> = 1035 sheep) and by a polymerase chain reaction (PCR) (<i>n</i> = 164 sheep) in China. The results showed that 75 sheep were seropositive (titer ≥ 1:100), with a prevalence of 7.2%, as assessed by MAT. The molecular prevalence of <i>T. gondii</i> in sheep was 16.4% (27/164), as assessed by PCR. From these 164 tissue or blood samples, 22 tissues and 2 blood samples were selected in order to isolate <i>T. gondii</i> by mouse bioassay. A viable <i>T. gondii</i> strain (TgSheepCHn15, ToxoDB #10) was isolated from sheep tissues in the veterinary clinic. This strain was avirulent for Swiss mice, and the survival time of mice was 97 ± 31 days. This is the first instance of the isolation of a Type I strain from sheep with avirulence in mice. The prevalence of <i>T. gondii</i> has been decreasing in sheep from China. However, <i>T. gondii</i> remains present in sheep herds and should not be ignored.https://www.mdpi.com/2076-2615/15/8/1074ovine abortiongenotypic diversityepidemiologyToxoDB # 10isolation |
| spellingShingle | Yurong Yang Yiheng Ma Kai Quan Bingyan Guo Yibao Jiang Toxoplasmosis in Sheep Caused by <i>Toxoplasma gondii</i> Clonal Type I Animals ovine abortion genotypic diversity epidemiology ToxoDB # 10 isolation |
| title | Toxoplasmosis in Sheep Caused by <i>Toxoplasma gondii</i> Clonal Type I |
| title_full | Toxoplasmosis in Sheep Caused by <i>Toxoplasma gondii</i> Clonal Type I |
| title_fullStr | Toxoplasmosis in Sheep Caused by <i>Toxoplasma gondii</i> Clonal Type I |
| title_full_unstemmed | Toxoplasmosis in Sheep Caused by <i>Toxoplasma gondii</i> Clonal Type I |
| title_short | Toxoplasmosis in Sheep Caused by <i>Toxoplasma gondii</i> Clonal Type I |
| title_sort | toxoplasmosis in sheep caused by i toxoplasma gondii i clonal type i |
| topic | ovine abortion genotypic diversity epidemiology ToxoDB # 10 isolation |
| url | https://www.mdpi.com/2076-2615/15/8/1074 |
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