A face-off between Smaug and Caspar modulates primordial germ cell count and identity in Drosophila embryos
Proper formation and specification of Primordial Germ Cells (PGCs) is of special significance as they gradually transform into Germline Stem Cells (GSCs) that are ultimately responsible for generating the gametes. Intriguingly, not only the PGCs constitute the only immortal cell type but several spe...
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Taylor & Francis Group
2025-12-01
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19336934.2024.2438473 |
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| author | Girish Deshpande Subhradip Das Adheena Elsa Roy Girish S Ratnaparkhi |
| author_facet | Girish Deshpande Subhradip Das Adheena Elsa Roy Girish S Ratnaparkhi |
| author_sort | Girish Deshpande |
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| description | Proper formation and specification of Primordial Germ Cells (PGCs) is of special significance as they gradually transform into Germline Stem Cells (GSCs) that are ultimately responsible for generating the gametes. Intriguingly, not only the PGCs constitute the only immortal cell type but several specific determinants also underlying PGC specification such as Vasa, Nanos and Germ-cell-less are conserved through evolution. In Drosophila melanogaster, PGC formation and specification depends on two independent factors, the maternally deposited specialized cytoplasm (or germ plasm) enriched in germline determinants, and the mechanisms that execute the even partitioning of these determinants between the daughter cells. Prior work has shown that Oskar protein is necessary and sufficient to assemble the functional germ plasm, whereas centrosomes associated with the nuclei that invade the germ plasm are responsible for its equitable distribution. Our recent data suggests that Caspar, the Drosophila orthologue of human Fas-associated factor-1 (FAF1) is a novel regulator that modulates both mechanisms that underlie the determination of PGC fate. Consistently, early blastoderm embryos derived from females compromised for caspar display reduced levels of Oskar and defective centrosomes. |
| format | Article |
| id | doaj-art-b10104bf6b374fb9a3ce2d929a8c6e59 |
| institution | Kabale University |
| issn | 1933-6934 1933-6942 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
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| series | Fly |
| spelling | doaj-art-b10104bf6b374fb9a3ce2d929a8c6e592025-01-08T08:54:08ZengTaylor & Francis GroupFly1933-69341933-69422025-12-0119110.1080/19336934.2024.2438473A face-off between Smaug and Caspar modulates primordial germ cell count and identity in Drosophila embryosGirish Deshpande0Subhradip Das1Adheena Elsa Roy2Girish S Ratnaparkhi3Department of Biology, Indian Institute of Science Education & Research, Pune, IndiaDepartment of Biology, Indian Institute of Science Education & Research, Pune, IndiaDepartment of Biology, Indian Institute of Science Education & Research, Pune, IndiaDepartment of Biology, Indian Institute of Science Education & Research, Pune, IndiaProper formation and specification of Primordial Germ Cells (PGCs) is of special significance as they gradually transform into Germline Stem Cells (GSCs) that are ultimately responsible for generating the gametes. Intriguingly, not only the PGCs constitute the only immortal cell type but several specific determinants also underlying PGC specification such as Vasa, Nanos and Germ-cell-less are conserved through evolution. In Drosophila melanogaster, PGC formation and specification depends on two independent factors, the maternally deposited specialized cytoplasm (or germ plasm) enriched in germline determinants, and the mechanisms that execute the even partitioning of these determinants between the daughter cells. Prior work has shown that Oskar protein is necessary and sufficient to assemble the functional germ plasm, whereas centrosomes associated with the nuclei that invade the germ plasm are responsible for its equitable distribution. Our recent data suggests that Caspar, the Drosophila orthologue of human Fas-associated factor-1 (FAF1) is a novel regulator that modulates both mechanisms that underlie the determination of PGC fate. Consistently, early blastoderm embryos derived from females compromised for caspar display reduced levels of Oskar and defective centrosomes.https://www.tandfonline.com/doi/10.1080/19336934.2024.2438473FAF1Drosophilagerm plasmmaternalPGCsOskar |
| spellingShingle | Girish Deshpande Subhradip Das Adheena Elsa Roy Girish S Ratnaparkhi A face-off between Smaug and Caspar modulates primordial germ cell count and identity in Drosophila embryos Fly FAF1 Drosophila germ plasm maternal PGCs Oskar |
| title | A face-off between Smaug and Caspar modulates primordial germ cell count and identity in Drosophila embryos |
| title_full | A face-off between Smaug and Caspar modulates primordial germ cell count and identity in Drosophila embryos |
| title_fullStr | A face-off between Smaug and Caspar modulates primordial germ cell count and identity in Drosophila embryos |
| title_full_unstemmed | A face-off between Smaug and Caspar modulates primordial germ cell count and identity in Drosophila embryos |
| title_short | A face-off between Smaug and Caspar modulates primordial germ cell count and identity in Drosophila embryos |
| title_sort | face off between smaug and caspar modulates primordial germ cell count and identity in drosophila embryos |
| topic | FAF1 Drosophila germ plasm maternal PGCs Oskar |
| url | https://www.tandfonline.com/doi/10.1080/19336934.2024.2438473 |
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