Urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathy
Objective The chronic kidney disease (CKD) is widely diagnosed on the basis of albuminuria and the glomerular filtration rate. A more precise diagnosis of CKD, however, requires the assessment of other factors. Urinary adiponectin recently attracted attention for CKD assessment, but evaluation is di...
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| Format: | Article |
| Language: | English |
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BMJ Publishing Group
2019-05-01
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| Series: | BMJ Open Diabetes Research & Care |
| Online Access: | https://drc.bmj.com/content/7/1/e000661.full |
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| author | Shinnosuke Yamakado Hiroki Cho Mikio Inada Mika Morikawa Yong-Huang Jiang Kenji Saito Kazunari Nakaishi Satoshi Watabe Hitomi Takagi Mugiho Kaneda Akira Nakatsuma Masaki Ninomiya Hitomi Imachi Takeshi Arai Takuo Yoshimoto Koji Murao Jyun-Hao Chang Shih-Min Chen Yi-Chen Shih Min-Jing Zeng Liang-Yin Ke Chu-Huang Chen Teruki Yoshimura Toshiaki Miura Etsuro Ito |
| author_facet | Shinnosuke Yamakado Hiroki Cho Mikio Inada Mika Morikawa Yong-Huang Jiang Kenji Saito Kazunari Nakaishi Satoshi Watabe Hitomi Takagi Mugiho Kaneda Akira Nakatsuma Masaki Ninomiya Hitomi Imachi Takeshi Arai Takuo Yoshimoto Koji Murao Jyun-Hao Chang Shih-Min Chen Yi-Chen Shih Min-Jing Zeng Liang-Yin Ke Chu-Huang Chen Teruki Yoshimura Toshiaki Miura Etsuro Ito |
| author_sort | Shinnosuke Yamakado |
| collection | DOAJ |
| description | Objective The chronic kidney disease (CKD) is widely diagnosed on the basis of albuminuria and the glomerular filtration rate. A more precise diagnosis of CKD, however, requires the assessment of other factors. Urinary adiponectin recently attracted attention for CKD assessment, but evaluation is difficult due to the very low concentration of urinary adiponectin in normal subjects.Research design and methods We developed an ultrasensitive ELISA coupled with thionicotinamide-adenine dinucleotide cycling to detect trace amounts of proteins, which allows us to measure urinary adiponectin at the subattomole level. We measured urinary adiponectin levels in 59 patients with diabetes mellitus (DM) and 24 subjects without DM (normal) to test our hypothesis that urinary adiponectin levels increase with progression of CKD due to DM.Results The urinary adiponectin levels were 14.88±3.16 (ng/mg creatinine, mean±SEM) for patients with DM, and 3.06±0.33 (ng/mg creatinine) for normal subjects. The threshold between them was 4.0 ng/mg creatinine. The urinary adiponectin levels increased with an increase in the CKD risk. Furthermore, urinary adiponectin mainly formed a medium-molecular weight multimer (a hexamer) in patients with DM, whereas it formed only a low-molecular weight multimer (a trimer) in normal subjects. That is, the increase in urinary adiponectin in patients with DM led to the emergence of a medium-molecular weight form in urine.Conclusions Our new assay showed that urinary adiponectin could be a new diagnostic index for CKD. This assay is a non-invasive test using only urine, thus reducing the patient burden. |
| format | Article |
| id | doaj-art-b100368e04c046eea405aea7cc3cf226 |
| institution | DOAJ |
| issn | 2052-4897 |
| language | English |
| publishDate | 2019-05-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | BMJ Open Diabetes Research & Care |
| spelling | doaj-art-b100368e04c046eea405aea7cc3cf2262025-08-20T02:49:02ZengBMJ Publishing GroupBMJ Open Diabetes Research & Care2052-48972019-05-017110.1136/bmjdrc-2019-000661Urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathyShinnosuke Yamakado0Hiroki Cho1Mikio Inada2Mika Morikawa3Yong-Huang Jiang4Kenji Saito5Kazunari Nakaishi6Satoshi Watabe7Hitomi Takagi8Mugiho Kaneda9Akira Nakatsuma10Masaki Ninomiya11Hitomi Imachi12Takeshi Arai13Takuo Yoshimoto14Koji Murao15Jyun-Hao Chang16Shih-Min Chen17Yi-Chen Shih18Min-Jing Zeng19Liang-Yin Ke20Chu-Huang Chen21Teruki Yoshimura22Toshiaki Miura23Etsuro Ito24Department of Biology, Waseda University, Shinjuku, Tokyo, JapanDepartment of Biology, Waseda University, Shinjuku, Tokyo, JapanDepartment of Biology, Waseda University, Shinjuku, Tokyo, JapanR&D Headquarters, TAUNS Laboratories, Izunokuni, Shizuoka, JapanR&D Headquarters, TAUNS Laboratories, Izunokuni, Shizuoka, Japan1 Department of Rheumatology, Fukushima Medical University School of Medicine, Fukushima, JapanR&D Headquarters, TAUNS Laboratories, Izunokuni, Shizuoka, JapanR&D Headquarters, TAUNS Laboratories, Izunokuni, Shizuoka, JapanKagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Sanuki, Kagawa, JapanKagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Sanuki, Kagawa, JapanKagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Sanuki, Kagawa, JapanKagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Sanuki, Kagawa, JapanFaculty of Medicine, Kagawa University, Miki, Kagawa, JapanFaculty of Medicine, Kagawa University, Miki, Kagawa, JapanFaculty of Medicine, Kagawa University, Miki, Kagawa, JapanFaculty of Medicine, Kagawa University, Miki, Kagawa, JapanLipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung, TaiwanLipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung, TaiwanLipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung, TaiwanLipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung, TaiwanLipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan5 Vascular and Medicinal Research, Texas Heart Institute, Houston, Texas, USAFaculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, JapanGraduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido, JapanDepartment of Biology, Waseda University, Shinjuku, Tokyo, JapanObjective The chronic kidney disease (CKD) is widely diagnosed on the basis of albuminuria and the glomerular filtration rate. A more precise diagnosis of CKD, however, requires the assessment of other factors. Urinary adiponectin recently attracted attention for CKD assessment, but evaluation is difficult due to the very low concentration of urinary adiponectin in normal subjects.Research design and methods We developed an ultrasensitive ELISA coupled with thionicotinamide-adenine dinucleotide cycling to detect trace amounts of proteins, which allows us to measure urinary adiponectin at the subattomole level. We measured urinary adiponectin levels in 59 patients with diabetes mellitus (DM) and 24 subjects without DM (normal) to test our hypothesis that urinary adiponectin levels increase with progression of CKD due to DM.Results The urinary adiponectin levels were 14.88±3.16 (ng/mg creatinine, mean±SEM) for patients with DM, and 3.06±0.33 (ng/mg creatinine) for normal subjects. The threshold between them was 4.0 ng/mg creatinine. The urinary adiponectin levels increased with an increase in the CKD risk. Furthermore, urinary adiponectin mainly formed a medium-molecular weight multimer (a hexamer) in patients with DM, whereas it formed only a low-molecular weight multimer (a trimer) in normal subjects. That is, the increase in urinary adiponectin in patients with DM led to the emergence of a medium-molecular weight form in urine.Conclusions Our new assay showed that urinary adiponectin could be a new diagnostic index for CKD. This assay is a non-invasive test using only urine, thus reducing the patient burden.https://drc.bmj.com/content/7/1/e000661.full |
| spellingShingle | Shinnosuke Yamakado Hiroki Cho Mikio Inada Mika Morikawa Yong-Huang Jiang Kenji Saito Kazunari Nakaishi Satoshi Watabe Hitomi Takagi Mugiho Kaneda Akira Nakatsuma Masaki Ninomiya Hitomi Imachi Takeshi Arai Takuo Yoshimoto Koji Murao Jyun-Hao Chang Shih-Min Chen Yi-Chen Shih Min-Jing Zeng Liang-Yin Ke Chu-Huang Chen Teruki Yoshimura Toshiaki Miura Etsuro Ito Urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathy BMJ Open Diabetes Research & Care |
| title | Urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathy |
| title_full | Urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathy |
| title_fullStr | Urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathy |
| title_full_unstemmed | Urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathy |
| title_short | Urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathy |
| title_sort | urinary adiponectin as a new diagnostic index for chronic kidney disease due to diabetic nephropathy |
| url | https://drc.bmj.com/content/7/1/e000661.full |
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