Revealing the Influence of Rootstock Choice on Clementine Mandarin Leaves and Peel Volatile Profile

This study investigates the impact of rootstock selection on the essential oil (EO) composition of clementine mandarin (<i>Citrus clementina</i> Hort. ex Tan.) var. SRA 63 cultivated in southern Greece. EOs were extracted from the peel and leaves of trees grafted on four commonly used ro...

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Main Authors: Vasileios Ziogas, Evgenia Panou, Konstantia Graikou, Christos Ganos, Evgenia Ntamposi, Ioanna Chinou
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Horticulturae
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Online Access:https://www.mdpi.com/2311-7524/11/5/523
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author Vasileios Ziogas
Evgenia Panou
Konstantia Graikou
Christos Ganos
Evgenia Ntamposi
Ioanna Chinou
author_facet Vasileios Ziogas
Evgenia Panou
Konstantia Graikou
Christos Ganos
Evgenia Ntamposi
Ioanna Chinou
author_sort Vasileios Ziogas
collection DOAJ
description This study investigates the impact of rootstock selection on the essential oil (EO) composition of clementine mandarin (<i>Citrus clementina</i> Hort. ex Tan.) var. SRA 63 cultivated in southern Greece. EOs were extracted from the peel and leaves of trees grafted on four commonly used rootstocks: Cleopatra mandarin, sour orange, Troyer citrange, and Swingle citrumelo. The GC-MS analysis revealed significant qualitative and quantitative differences in EO profiles across the different rootstock combinations. In peel EOs, limonene was the dominant compound, particularly in trees grafted onto Cleopatra mandarin and Swingle citrumelo, while Troyer citrange favored a more diverse chemical profile rich in oxygenated monoterpenes, sesquiterpenes, and aldehydes. Leaf EOs were characterized by high levels of sabinene, linalool, and limonene, with Swingle citrumelo promoting sabinene production and Troyer citrange enhancing limonene content and sesquiterpene diversity. Sour orange showed an intermediate effect, increasing both compound diversity and abundance. These results underscore the critical role played by rootstock in modulating the biosynthesis of volatile compounds, likely through physiological and molecular interactions with the scion. These findings offer valuable insights into optimizing EO yield and quality in citriculture and support the broader goal of valorizing <i>Citrus</i> by-products through targeted agricultural practices. This research contributes to the implementation of targeted agricultural practices (rootstock choice) for the development of high-value <i>Citrus</i>-based products with specific quality traits.
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spelling doaj-art-b0fcab09d9f642a194c3c4692db027e72025-08-20T01:56:31ZengMDPI AGHorticulturae2311-75242025-05-0111552310.3390/horticulturae11050523Revealing the Influence of Rootstock Choice on Clementine Mandarin Leaves and Peel Volatile ProfileVasileios Ziogas0Evgenia Panou1Konstantia Graikou2Christos Ganos3Evgenia Ntamposi4Ioanna Chinou5Institute of Olive Tree, Subtropical Plants and Viticulture, Hellenic Agricultural Organization-DIMITRA (ELGO-DIMITRA), 73134 Chania, GreeceLab of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National & Kapodistrian University of Athens, 15771 Athens, GreeceLab of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National & Kapodistrian University of Athens, 15771 Athens, GreeceLab of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National & Kapodistrian University of Athens, 15771 Athens, GreeceMinistry of Rural Development and Food, 11143 Athens, GreeceLab of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National & Kapodistrian University of Athens, 15771 Athens, GreeceThis study investigates the impact of rootstock selection on the essential oil (EO) composition of clementine mandarin (<i>Citrus clementina</i> Hort. ex Tan.) var. SRA 63 cultivated in southern Greece. EOs were extracted from the peel and leaves of trees grafted on four commonly used rootstocks: Cleopatra mandarin, sour orange, Troyer citrange, and Swingle citrumelo. The GC-MS analysis revealed significant qualitative and quantitative differences in EO profiles across the different rootstock combinations. In peel EOs, limonene was the dominant compound, particularly in trees grafted onto Cleopatra mandarin and Swingle citrumelo, while Troyer citrange favored a more diverse chemical profile rich in oxygenated monoterpenes, sesquiterpenes, and aldehydes. Leaf EOs were characterized by high levels of sabinene, linalool, and limonene, with Swingle citrumelo promoting sabinene production and Troyer citrange enhancing limonene content and sesquiterpene diversity. Sour orange showed an intermediate effect, increasing both compound diversity and abundance. These results underscore the critical role played by rootstock in modulating the biosynthesis of volatile compounds, likely through physiological and molecular interactions with the scion. These findings offer valuable insights into optimizing EO yield and quality in citriculture and support the broader goal of valorizing <i>Citrus</i> by-products through targeted agricultural practices. This research contributes to the implementation of targeted agricultural practices (rootstock choice) for the development of high-value <i>Citrus</i>-based products with specific quality traits.https://www.mdpi.com/2311-7524/11/5/523<i>Citrus</i>clementineSRA 63essential oilsrootstockCleopatra
spellingShingle Vasileios Ziogas
Evgenia Panou
Konstantia Graikou
Christos Ganos
Evgenia Ntamposi
Ioanna Chinou
Revealing the Influence of Rootstock Choice on Clementine Mandarin Leaves and Peel Volatile Profile
Horticulturae
<i>Citrus</i>
clementine
SRA 63
essential oils
rootstock
Cleopatra
title Revealing the Influence of Rootstock Choice on Clementine Mandarin Leaves and Peel Volatile Profile
title_full Revealing the Influence of Rootstock Choice on Clementine Mandarin Leaves and Peel Volatile Profile
title_fullStr Revealing the Influence of Rootstock Choice on Clementine Mandarin Leaves and Peel Volatile Profile
title_full_unstemmed Revealing the Influence of Rootstock Choice on Clementine Mandarin Leaves and Peel Volatile Profile
title_short Revealing the Influence of Rootstock Choice on Clementine Mandarin Leaves and Peel Volatile Profile
title_sort revealing the influence of rootstock choice on clementine mandarin leaves and peel volatile profile
topic <i>Citrus</i>
clementine
SRA 63
essential oils
rootstock
Cleopatra
url https://www.mdpi.com/2311-7524/11/5/523
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