Synergy between HDAC and PARP Inhibitors on Proliferation of a Human Anaplastic Thyroid Cancer-Derived Cell Line
Anaplastic thyroid carcinoma (ATC) is a very aggressive human malignancy, having a marked degree of invasiveness and no features of thyroid differentiation. It is known that either HDAC inhibitors or PARP inhibitors have antiproliferative effects on thyroid cancer cells. Therefore, in this study the...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2015/978371 |
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| author | Federica Baldan Catia Mio Lorenzo Allegri Cinzia Puppin Diego Russo Sebastiano Filetti Giuseppe Damante |
| author_facet | Federica Baldan Catia Mio Lorenzo Allegri Cinzia Puppin Diego Russo Sebastiano Filetti Giuseppe Damante |
| author_sort | Federica Baldan |
| collection | DOAJ |
| description | Anaplastic thyroid carcinoma (ATC) is a very aggressive human malignancy, having a marked degree of invasiveness and no features of thyroid differentiation. It is known that either HDAC inhibitors or PARP inhibitors have antiproliferative effects on thyroid cancer cells. Therefore, in this study the possible synergy between the two types of compounds has been investigated. The ATC-derived cell line SW1736 has been treated with the HDAC inhibitor suberoylanilide hydroxamic acid (SAHA) and the PARP inhibitor PJ34, alone or in combination. In terms of cell viability, the combination index value was always lower than 1 at various tested dosages, indicating, therefore, synergy in a wide range of doses for both compounds. Synergy was also observed in induction of apoptosis. In terms of thyroid-specific gene expression, synergy was observed for TSHR mRNA levels but not for NIS, TTF1, TTF2, and PAX8 mRNA levels. Altogether, these data suggest that the combined use of HDAC and PARP inhibitors may be a useful strategy for treatment of ATC. |
| format | Article |
| id | doaj-art-b0fbff6418d946359ecb64ddb98c0be4 |
| institution | OA Journals |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-b0fbff6418d946359ecb64ddb98c0be42025-08-20T02:23:36ZengWileyInternational Journal of Endocrinology1687-83371687-83452015-01-01201510.1155/2015/978371978371Synergy between HDAC and PARP Inhibitors on Proliferation of a Human Anaplastic Thyroid Cancer-Derived Cell LineFederica Baldan0Catia Mio1Lorenzo Allegri2Cinzia Puppin3Diego Russo4Sebastiano Filetti5Giuseppe Damante6Department of Medical and Biological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine, ItalyDepartment of Medical and Biological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine, ItalyDepartment of Medical and Biological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine, ItalyDepartment of Medical and Biological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine, ItalyDepartment of Health Sciences, University “Magna Graecia” of Catanzaro, 88100 Catanzaro, ItalyDepartment of Internal Medicine and Medical Specialties, University of Roma “La Sapienza”, 00198 Rome, ItalyDepartment of Medical and Biological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine, ItalyAnaplastic thyroid carcinoma (ATC) is a very aggressive human malignancy, having a marked degree of invasiveness and no features of thyroid differentiation. It is known that either HDAC inhibitors or PARP inhibitors have antiproliferative effects on thyroid cancer cells. Therefore, in this study the possible synergy between the two types of compounds has been investigated. The ATC-derived cell line SW1736 has been treated with the HDAC inhibitor suberoylanilide hydroxamic acid (SAHA) and the PARP inhibitor PJ34, alone or in combination. In terms of cell viability, the combination index value was always lower than 1 at various tested dosages, indicating, therefore, synergy in a wide range of doses for both compounds. Synergy was also observed in induction of apoptosis. In terms of thyroid-specific gene expression, synergy was observed for TSHR mRNA levels but not for NIS, TTF1, TTF2, and PAX8 mRNA levels. Altogether, these data suggest that the combined use of HDAC and PARP inhibitors may be a useful strategy for treatment of ATC.http://dx.doi.org/10.1155/2015/978371 |
| spellingShingle | Federica Baldan Catia Mio Lorenzo Allegri Cinzia Puppin Diego Russo Sebastiano Filetti Giuseppe Damante Synergy between HDAC and PARP Inhibitors on Proliferation of a Human Anaplastic Thyroid Cancer-Derived Cell Line International Journal of Endocrinology |
| title | Synergy between HDAC and PARP Inhibitors on Proliferation of a Human Anaplastic Thyroid Cancer-Derived Cell Line |
| title_full | Synergy between HDAC and PARP Inhibitors on Proliferation of a Human Anaplastic Thyroid Cancer-Derived Cell Line |
| title_fullStr | Synergy between HDAC and PARP Inhibitors on Proliferation of a Human Anaplastic Thyroid Cancer-Derived Cell Line |
| title_full_unstemmed | Synergy between HDAC and PARP Inhibitors on Proliferation of a Human Anaplastic Thyroid Cancer-Derived Cell Line |
| title_short | Synergy between HDAC and PARP Inhibitors on Proliferation of a Human Anaplastic Thyroid Cancer-Derived Cell Line |
| title_sort | synergy between hdac and parp inhibitors on proliferation of a human anaplastic thyroid cancer derived cell line |
| url | http://dx.doi.org/10.1155/2015/978371 |
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