A Novel and Robust Method for Investigating Fungal Biofilm
Candida auris, labeled an urgent threat by the CDC, shows significant resilience to treatments and disinfectants via biofilm formation, complicating treatment/disease management. The inconsistencies in biofilm architecture observed across studies hinder the understanding of its role in pathogenesis....
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2025-01-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5146&type=0 |
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| author | Biswambhar Biswas Shumaiza Asif Rekha Puria Anil Thakur |
| author_facet | Biswambhar Biswas Shumaiza Asif Rekha Puria Anil Thakur |
| author_sort | Biswambhar Biswas |
| collection | DOAJ |
| description | Candida auris, labeled an urgent threat by the CDC, shows significant resilience to treatments and disinfectants via biofilm formation, complicating treatment/disease management. The inconsistencies in biofilm architecture observed across studies hinder the understanding of its role in pathogenesis. Our novel in vitro technique cultivates C. auris biofilms on gelatin-coated coverslips, reliably producing multilayer biofilms with extracellular polymeric substances (EPS). This method, applicable to other Candida species like C. glabrata and C. albicans, is cost-effective and mimics the niche of biofilm formation. It is suitable for high-throughput drug screening and repurposing efforts, aiding in the development of new therapeutics. Our technique represents a significant advancement in Candida biofilm research, addressing the need for consistent, reproducible biofilm models. We detail a step-by-step procedure for creating a substratum for biofilm growth and measuring biofilm thickness using confocal laser scanning microscopy (CLSM) and ultrastructure by scanning electron microscopy (SEM). This method provides consistent outcomes across various Candida species. |
| format | Article |
| id | doaj-art-b0f4f9ae43ce45db80826193e7be9018 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-b0f4f9ae43ce45db80826193e7be90182025-02-07T08:16:31ZengBio-protocol LLCBio-Protocol2331-83252025-01-0115110.21769/BioProtoc.5146A Novel and Robust Method for Investigating Fungal BiofilmBiswambhar Biswas0Shumaiza Asif1Rekha Puria2Anil Thakur3Laboratory of Protein Translation and Fungal Pathogenesis, Regional Centre for Biotechnology, Faridabad, IndiaLaboratory of Protein Translation and Fungal Pathogenesis, Regional Centre for Biotechnology, Faridabad, IndiaDepartment of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, IndiaLaboratory of Protein Translation and Fungal Pathogenesis, Regional Centre for Biotechnology, Faridabad, IndiaCandida auris, labeled an urgent threat by the CDC, shows significant resilience to treatments and disinfectants via biofilm formation, complicating treatment/disease management. The inconsistencies in biofilm architecture observed across studies hinder the understanding of its role in pathogenesis. Our novel in vitro technique cultivates C. auris biofilms on gelatin-coated coverslips, reliably producing multilayer biofilms with extracellular polymeric substances (EPS). This method, applicable to other Candida species like C. glabrata and C. albicans, is cost-effective and mimics the niche of biofilm formation. It is suitable for high-throughput drug screening and repurposing efforts, aiding in the development of new therapeutics. Our technique represents a significant advancement in Candida biofilm research, addressing the need for consistent, reproducible biofilm models. We detail a step-by-step procedure for creating a substratum for biofilm growth and measuring biofilm thickness using confocal laser scanning microscopy (CLSM) and ultrastructure by scanning electron microscopy (SEM). This method provides consistent outcomes across various Candida species.https://bio-protocol.org/en/bpdetail?id=5146&type=0 |
| spellingShingle | Biswambhar Biswas Shumaiza Asif Rekha Puria Anil Thakur A Novel and Robust Method for Investigating Fungal Biofilm Bio-Protocol |
| title | A Novel and Robust Method for Investigating Fungal Biofilm |
| title_full | A Novel and Robust Method for Investigating Fungal Biofilm |
| title_fullStr | A Novel and Robust Method for Investigating Fungal Biofilm |
| title_full_unstemmed | A Novel and Robust Method for Investigating Fungal Biofilm |
| title_short | A Novel and Robust Method for Investigating Fungal Biofilm |
| title_sort | novel and robust method for investigating fungal biofilm |
| url | https://bio-protocol.org/en/bpdetail?id=5146&type=0 |
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