Stimulation of Rabbit Squamous Epidermis Cells Using Extracts of Mistletoe Dendrophthoe pentandra L. Miq in a Topical Gel
Quercetin-like compounds (QLCs) are secondary metabolite compounds of flavonol found in the leaf extract from the mistletoe Dendrophthoe pentandra L. Miq. This study aims to determine the ability of QLC to stimulate epithelialization in rabbit skin. The leaves were macerated with methanol, ethyl ace...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/vmi/4081052 |
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| author | Lazuardi Mochamad Chi-Hsien Chien Jie-Long He Eka Pramyrtha Hestianah Hani Plumeriastuti |
| author_facet | Lazuardi Mochamad Chi-Hsien Chien Jie-Long He Eka Pramyrtha Hestianah Hani Plumeriastuti |
| author_sort | Lazuardi Mochamad |
| collection | DOAJ |
| description | Quercetin-like compounds (QLCs) are secondary metabolite compounds of flavonol found in the leaf extract from the mistletoe Dendrophthoe pentandra L. Miq. This study aims to determine the ability of QLC to stimulate epithelialization in rabbit skin. The leaves were macerated with methanol, ethyl acetate, and n-hexane solvents, and crude macerates were separated and purified into QLC using preparative high-performance liquid chromatography. The purified QLC as an analyte was prepared in serial concentrations of 4.5%, 6%, 8%, and 10% and then formulated by new methods as topical gel preparations. The epithelialization stimulation test was performed on 60 rabbits divided into 20 rabbits for the trial group, the other 20 rabbits for the positive control group, and negative control groups. The trial group was split into 4 trial subgroups for topical gel application at each concentration. Gels were topically applied to the exfoliated skin of rabbits in the trial group twice a day for 5 days. The probit analysis showed that an epithelialization of 25%, 50%, and 75% of the in vivo study endpoint was found at a QLC concentration of 4.644%, 5.185%, and 5.790%. Rectangular cuboidal QLC particles with an average size of 0.01 μm–0.1 μm have shown the ability to stimulate epidermal epithelial proliferation starting from a concentration of 4.5% in topical gels with new formulations that can boost the epithelialization of the upper layers of the skin (p<0.05). |
| format | Article |
| id | doaj-art-b0f12aee66e3406ca90d05fc2029eeb6 |
| institution | DOAJ |
| issn | 2042-0048 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-b0f12aee66e3406ca90d05fc2029eeb62025-08-20T03:16:39ZengWileyVeterinary Medicine International2042-00482025-01-01202510.1155/vmi/4081052Stimulation of Rabbit Squamous Epidermis Cells Using Extracts of Mistletoe Dendrophthoe pentandra L. Miq in a Topical GelLazuardi Mochamad0Chi-Hsien Chien1Jie-Long He2Eka Pramyrtha Hestianah3Hani Plumeriastuti4Sub-Division Veterinary Pharmacy ScienceDepartment of Post-Baccalaureate Veterinary MedicineDepartment of Post-Baccalaureate Veterinary MedicineSub-Division Veterinary HistologySub-Division Veterinary HistologyQuercetin-like compounds (QLCs) are secondary metabolite compounds of flavonol found in the leaf extract from the mistletoe Dendrophthoe pentandra L. Miq. This study aims to determine the ability of QLC to stimulate epithelialization in rabbit skin. The leaves were macerated with methanol, ethyl acetate, and n-hexane solvents, and crude macerates were separated and purified into QLC using preparative high-performance liquid chromatography. The purified QLC as an analyte was prepared in serial concentrations of 4.5%, 6%, 8%, and 10% and then formulated by new methods as topical gel preparations. The epithelialization stimulation test was performed on 60 rabbits divided into 20 rabbits for the trial group, the other 20 rabbits for the positive control group, and negative control groups. The trial group was split into 4 trial subgroups for topical gel application at each concentration. Gels were topically applied to the exfoliated skin of rabbits in the trial group twice a day for 5 days. The probit analysis showed that an epithelialization of 25%, 50%, and 75% of the in vivo study endpoint was found at a QLC concentration of 4.644%, 5.185%, and 5.790%. Rectangular cuboidal QLC particles with an average size of 0.01 μm–0.1 μm have shown the ability to stimulate epidermal epithelial proliferation starting from a concentration of 4.5% in topical gels with new formulations that can boost the epithelialization of the upper layers of the skin (p<0.05).http://dx.doi.org/10.1155/vmi/4081052 |
| spellingShingle | Lazuardi Mochamad Chi-Hsien Chien Jie-Long He Eka Pramyrtha Hestianah Hani Plumeriastuti Stimulation of Rabbit Squamous Epidermis Cells Using Extracts of Mistletoe Dendrophthoe pentandra L. Miq in a Topical Gel Veterinary Medicine International |
| title | Stimulation of Rabbit Squamous Epidermis Cells Using Extracts of Mistletoe Dendrophthoe pentandra L. Miq in a Topical Gel |
| title_full | Stimulation of Rabbit Squamous Epidermis Cells Using Extracts of Mistletoe Dendrophthoe pentandra L. Miq in a Topical Gel |
| title_fullStr | Stimulation of Rabbit Squamous Epidermis Cells Using Extracts of Mistletoe Dendrophthoe pentandra L. Miq in a Topical Gel |
| title_full_unstemmed | Stimulation of Rabbit Squamous Epidermis Cells Using Extracts of Mistletoe Dendrophthoe pentandra L. Miq in a Topical Gel |
| title_short | Stimulation of Rabbit Squamous Epidermis Cells Using Extracts of Mistletoe Dendrophthoe pentandra L. Miq in a Topical Gel |
| title_sort | stimulation of rabbit squamous epidermis cells using extracts of mistletoe dendrophthoe pentandra l miq in a topical gel |
| url | http://dx.doi.org/10.1155/vmi/4081052 |
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