A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice
Abstract Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reade...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-12-01
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| Series: | npj Science of Food |
| Online Access: | https://doi.org/10.1038/s41538-024-00342-2 |
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| author | Saowalak Adunphatcharaphon Oluwatobi Kolawole Thanasat Sooksimuang Waraporn Panchan Wannee Wasuthep Anuwut Petdum Grit Pichayawaytin Rungroj Jintamethasawat Narusorn Doljirapisit Armote Somboonkaew Prae Noppakuadrittidej Sudtida Kaew-amdee Manlika Makornwattana Julie Meneely Christopher T. Elliott Awanwee Petchkongkaew Nitsara Karoonuthaisiri |
| author_facet | Saowalak Adunphatcharaphon Oluwatobi Kolawole Thanasat Sooksimuang Waraporn Panchan Wannee Wasuthep Anuwut Petdum Grit Pichayawaytin Rungroj Jintamethasawat Narusorn Doljirapisit Armote Somboonkaew Prae Noppakuadrittidej Sudtida Kaew-amdee Manlika Makornwattana Julie Meneely Christopher T. Elliott Awanwee Petchkongkaew Nitsara Karoonuthaisiri |
| author_sort | Saowalak Adunphatcharaphon |
| collection | DOAJ |
| description | Abstract Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins—aflatoxin B1, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B1 in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77% to 127%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice. |
| format | Article |
| id | doaj-art-b0e7cdd080004392b15112d80025f8a9 |
| institution | Kabale University |
| issn | 2396-8370 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Science of Food |
| spelling | doaj-art-b0e7cdd080004392b15112d80025f8a92025-01-05T12:47:43ZengNature Portfolionpj Science of Food2396-83702024-12-018111210.1038/s41538-024-00342-2A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in riceSaowalak Adunphatcharaphon0Oluwatobi Kolawole1Thanasat Sooksimuang2Waraporn Panchan3Wannee Wasuthep4Anuwut Petdum5Grit Pichayawaytin6Rungroj Jintamethasawat7Narusorn Doljirapisit8Armote Somboonkaew9Prae Noppakuadrittidej10Sudtida Kaew-amdee11Manlika Makornwattana12Julie Meneely13Christopher T. Elliott14Awanwee Petchkongkaew15Nitsara Karoonuthaisiri16International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)Institute for Global Food Security, Queen’s University BelfastInternational Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)International Joint Research Center on Food Security (IJC-FOODSEC)Abstract Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins—aflatoxin B1, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B1 in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77% to 127%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice.https://doi.org/10.1038/s41538-024-00342-2 |
| spellingShingle | Saowalak Adunphatcharaphon Oluwatobi Kolawole Thanasat Sooksimuang Waraporn Panchan Wannee Wasuthep Anuwut Petdum Grit Pichayawaytin Rungroj Jintamethasawat Narusorn Doljirapisit Armote Somboonkaew Prae Noppakuadrittidej Sudtida Kaew-amdee Manlika Makornwattana Julie Meneely Christopher T. Elliott Awanwee Petchkongkaew Nitsara Karoonuthaisiri A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice npj Science of Food |
| title | A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice |
| title_full | A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice |
| title_fullStr | A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice |
| title_full_unstemmed | A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice |
| title_short | A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice |
| title_sort | multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice |
| url | https://doi.org/10.1038/s41538-024-00342-2 |
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