A Transcriptomic Signature of Mouse Liver Progenitor Cells
Liver progenitor cells (LPCs) can proliferate extensively, are able to differentiate into hepatocytes and cholangiocytes, and contribute to liver regeneration. The presence of LPCs, however, often accompanies liver disease and hepatocellular carcinoma (HCC), indicating that they may be a cancer stem...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/5702873 |
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| author | Adam M. Passman Jasmine Low Roslyn London Janina E. E. Tirnitz-Parker Atsushi Miyajima Minoru Tanaka Helene Strick-Marchand Gretchen J. Darlington Megan Finch-Edmondson Scott Ochsner Cornelia Zhu James Whelan Bernard A. Callus George C. T. Yeoh |
| author_facet | Adam M. Passman Jasmine Low Roslyn London Janina E. E. Tirnitz-Parker Atsushi Miyajima Minoru Tanaka Helene Strick-Marchand Gretchen J. Darlington Megan Finch-Edmondson Scott Ochsner Cornelia Zhu James Whelan Bernard A. Callus George C. T. Yeoh |
| author_sort | Adam M. Passman |
| collection | DOAJ |
| description | Liver progenitor cells (LPCs) can proliferate extensively, are able to differentiate into hepatocytes and cholangiocytes, and contribute to liver regeneration. The presence of LPCs, however, often accompanies liver disease and hepatocellular carcinoma (HCC), indicating that they may be a cancer stem cell. Understanding LPC biology and establishing a sensitive, rapid, and reliable method to detect their presence in the liver will assist diagnosis and facilitate monitoring of treatment outcomes in patients with liver pathologies. A transcriptomic meta-analysis of over 400 microarrays was undertaken to compare LPC lines against datasets of muscle and embryonic stem cell lines, embryonic and developed liver (DL), and HCC. Three gene clusters distinguishing LPCs from other liver cell types were identified. Pathways overrepresented in these clusters denote the proliferative nature of LPCs and their association with HCC. Our analysis also revealed 26 novel markers, LPC markers, including Mcm2 and Ltbp3, and eight known LPC markers, including M2pk and Ncam. These markers specified the presence of LPCs in pathological liver tissue by qPCR and correlated with LPC abundance determined using immunohistochemistry. These results showcase the value of global transcript profiling to identify pathways and markers that may be used to detect LPCs in injured or diseased liver. |
| format | Article |
| id | doaj-art-b0dfd949a72441b68d7de746f42adaad |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b0dfd949a72441b68d7de746f42adaad2025-08-20T02:23:34ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/57028735702873A Transcriptomic Signature of Mouse Liver Progenitor CellsAdam M. Passman0Jasmine Low1Roslyn London2Janina E. E. Tirnitz-Parker3Atsushi Miyajima4Minoru Tanaka5Helene Strick-Marchand6Gretchen J. Darlington7Megan Finch-Edmondson8Scott Ochsner9Cornelia Zhu10James Whelan11Bernard A. Callus12George C. T. Yeoh13School of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, AustraliaSchool of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, AustraliaSchool of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, AustraliaSchool of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, AustraliaInstitute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-8654, JapanInstitute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-8654, JapanUnité de Génétique de la Différenciation, Institut Pasteur, 75015 Paris, FranceHuffington Center on Aging, Baylor College of Medicine, Houston, TX 77030, USADepartment of Physiology, NUS Yong Loo Lin School of Medicine, 117411, SingaporeDepartment of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USASchool of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, AustraliaARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Crawley, WA 6009, AustraliaSchool of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, AustraliaSchool of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, AustraliaLiver progenitor cells (LPCs) can proliferate extensively, are able to differentiate into hepatocytes and cholangiocytes, and contribute to liver regeneration. The presence of LPCs, however, often accompanies liver disease and hepatocellular carcinoma (HCC), indicating that they may be a cancer stem cell. Understanding LPC biology and establishing a sensitive, rapid, and reliable method to detect their presence in the liver will assist diagnosis and facilitate monitoring of treatment outcomes in patients with liver pathologies. A transcriptomic meta-analysis of over 400 microarrays was undertaken to compare LPC lines against datasets of muscle and embryonic stem cell lines, embryonic and developed liver (DL), and HCC. Three gene clusters distinguishing LPCs from other liver cell types were identified. Pathways overrepresented in these clusters denote the proliferative nature of LPCs and their association with HCC. Our analysis also revealed 26 novel markers, LPC markers, including Mcm2 and Ltbp3, and eight known LPC markers, including M2pk and Ncam. These markers specified the presence of LPCs in pathological liver tissue by qPCR and correlated with LPC abundance determined using immunohistochemistry. These results showcase the value of global transcript profiling to identify pathways and markers that may be used to detect LPCs in injured or diseased liver.http://dx.doi.org/10.1155/2016/5702873 |
| spellingShingle | Adam M. Passman Jasmine Low Roslyn London Janina E. E. Tirnitz-Parker Atsushi Miyajima Minoru Tanaka Helene Strick-Marchand Gretchen J. Darlington Megan Finch-Edmondson Scott Ochsner Cornelia Zhu James Whelan Bernard A. Callus George C. T. Yeoh A Transcriptomic Signature of Mouse Liver Progenitor Cells Stem Cells International |
| title | A Transcriptomic Signature of Mouse Liver Progenitor Cells |
| title_full | A Transcriptomic Signature of Mouse Liver Progenitor Cells |
| title_fullStr | A Transcriptomic Signature of Mouse Liver Progenitor Cells |
| title_full_unstemmed | A Transcriptomic Signature of Mouse Liver Progenitor Cells |
| title_short | A Transcriptomic Signature of Mouse Liver Progenitor Cells |
| title_sort | transcriptomic signature of mouse liver progenitor cells |
| url | http://dx.doi.org/10.1155/2016/5702873 |
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