Evaluation of infrapatellar fat pad elasticity in knee osteoarthritis using IVIM-DWI-based virtual MR elastography: repeatability and reproducibility analysis

Abstract Background Fibrosis of the infrapatellar fat pad (IPFP) leads to changes in its stiffness, which may impact knee osteoarthritis. However, few studies have utilized virtual MR elastography to assess the variations of the IPFP. This study aimed to evaluate the value of intravoxel incoherent m...

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Main Authors: Hui Tan, Qiuju Fan, Yong Yu, Nan Yu, Shaoyu Wang, Shunlin Guo
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Musculoskeletal Disorders
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Online Access:https://doi.org/10.1186/s12891-025-08660-6
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author Hui Tan
Qiuju Fan
Yong Yu
Nan Yu
Shaoyu Wang
Shunlin Guo
author_facet Hui Tan
Qiuju Fan
Yong Yu
Nan Yu
Shaoyu Wang
Shunlin Guo
author_sort Hui Tan
collection DOAJ
description Abstract Background Fibrosis of the infrapatellar fat pad (IPFP) leads to changes in its stiffness, which may impact knee osteoarthritis. However, few studies have utilized virtual MR elastography to assess the variations of the IPFP. This study aimed to evaluate the value of intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI)-based virtual MR elastography (vMRE) in the IPFP by assessing the test-retest repeatability, as well as intra- and inter-observer reproducibility. Methods A total of 71 subjects underwent IVIM-DWI examinations, which were conducted twice with an interval of 30–60 min using an 18-channel knee coil at 3T. Shifted apparent diffusion coefficient (sADC) was calculated from two different sets of b-values (b = 200/800 sec/mm2 and 200/1500 sec/mm2) and then converted to IVIM-DWI MRI-based virtual shear modulus (μdiff_800 and μdiff_1500). Two readers independently delineated regions of interest (ROI) within the IPFP on the vMRE stiffness map to obtain the mean and standard deviation (SD) values of μdiff. Short-term test-retest repeatability, as well as intra- and inter-observer agreement were assessed using the intra-class correlation coefficient (ICC), the coefficient of variation (CoV), and Bland-Altman limits of agreement (LoA). Results The mean and SD values of μdiff_1500, along with the mean value of μdiff_800 exhibited excellent intra- and inter-observer reproducibility agreement (ICC ≥ 0.90 and CoV ≤ 10%, P˂ 0.001). The intra- and inter-observer ICCs for the mean values of μdiff_800 were 0.917 and 0.901, respectively, while the ICCs for the SD values of μdiff_800 were 0.870 and 0.863, with CoV exceeding 10% (P˂ 0.001). The test-retest repeatability of the average value of μdiff_1500 was excellent (ICC = 0.902; CoV = 6.8%) compared to μdiff_800 (ICC = 0.877; CoV = 15.3%). Test–retest repeatability of SD for μdiff_1500 was good (ICC = 0.803; CoV = 11.5%) in comparison to SD for μdiff_800 (ICC = 0.796; CoV = 13.5%). Conclusions IVIM-DWI-based vMRE demonstrated significant potential as a reliable tool for measuring tissue elasticity in the IPFP, exhibiting higher repeatability for μdiff_1500 than for μdiff_800.
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spelling doaj-art-b0dc629ab0d54010bf3122818b9b7be82025-08-20T02:30:14ZengBMCBMC Musculoskeletal Disorders1471-24742025-04-0126111210.1186/s12891-025-08660-6Evaluation of infrapatellar fat pad elasticity in knee osteoarthritis using IVIM-DWI-based virtual MR elastography: repeatability and reproducibility analysisHui Tan0Qiuju Fan1Yong Yu2Nan Yu3Shaoyu Wang4Shunlin Guo5The First School of Clinical Medicine, Lanzhou UniversityDepartment of Radiology, Affiliated Hospital of Shaanxi University of Chinese MedicineDepartment of Radiology, Affiliated Hospital of Shaanxi University of Chinese MedicineDepartment of Radiology, Affiliated Hospital of Shaanxi University of Chinese MedicineMR Senior Scientific Marketing Specialist, Siemens HealthineersThe First School of Clinical Medicine, Lanzhou UniversityAbstract Background Fibrosis of the infrapatellar fat pad (IPFP) leads to changes in its stiffness, which may impact knee osteoarthritis. However, few studies have utilized virtual MR elastography to assess the variations of the IPFP. This study aimed to evaluate the value of intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI)-based virtual MR elastography (vMRE) in the IPFP by assessing the test-retest repeatability, as well as intra- and inter-observer reproducibility. Methods A total of 71 subjects underwent IVIM-DWI examinations, which were conducted twice with an interval of 30–60 min using an 18-channel knee coil at 3T. Shifted apparent diffusion coefficient (sADC) was calculated from two different sets of b-values (b = 200/800 sec/mm2 and 200/1500 sec/mm2) and then converted to IVIM-DWI MRI-based virtual shear modulus (μdiff_800 and μdiff_1500). Two readers independently delineated regions of interest (ROI) within the IPFP on the vMRE stiffness map to obtain the mean and standard deviation (SD) values of μdiff. Short-term test-retest repeatability, as well as intra- and inter-observer agreement were assessed using the intra-class correlation coefficient (ICC), the coefficient of variation (CoV), and Bland-Altman limits of agreement (LoA). Results The mean and SD values of μdiff_1500, along with the mean value of μdiff_800 exhibited excellent intra- and inter-observer reproducibility agreement (ICC ≥ 0.90 and CoV ≤ 10%, P˂ 0.001). The intra- and inter-observer ICCs for the mean values of μdiff_800 were 0.917 and 0.901, respectively, while the ICCs for the SD values of μdiff_800 were 0.870 and 0.863, with CoV exceeding 10% (P˂ 0.001). The test-retest repeatability of the average value of μdiff_1500 was excellent (ICC = 0.902; CoV = 6.8%) compared to μdiff_800 (ICC = 0.877; CoV = 15.3%). Test–retest repeatability of SD for μdiff_1500 was good (ICC = 0.803; CoV = 11.5%) in comparison to SD for μdiff_800 (ICC = 0.796; CoV = 13.5%). Conclusions IVIM-DWI-based vMRE demonstrated significant potential as a reliable tool for measuring tissue elasticity in the IPFP, exhibiting higher repeatability for μdiff_1500 than for μdiff_800.https://doi.org/10.1186/s12891-025-08660-6Intravoxel incoherent motionDiffusion-weighted imagingElastographyOsteoarthritisInfrapatellar fat pad
spellingShingle Hui Tan
Qiuju Fan
Yong Yu
Nan Yu
Shaoyu Wang
Shunlin Guo
Evaluation of infrapatellar fat pad elasticity in knee osteoarthritis using IVIM-DWI-based virtual MR elastography: repeatability and reproducibility analysis
BMC Musculoskeletal Disorders
Intravoxel incoherent motion
Diffusion-weighted imaging
Elastography
Osteoarthritis
Infrapatellar fat pad
title Evaluation of infrapatellar fat pad elasticity in knee osteoarthritis using IVIM-DWI-based virtual MR elastography: repeatability and reproducibility analysis
title_full Evaluation of infrapatellar fat pad elasticity in knee osteoarthritis using IVIM-DWI-based virtual MR elastography: repeatability and reproducibility analysis
title_fullStr Evaluation of infrapatellar fat pad elasticity in knee osteoarthritis using IVIM-DWI-based virtual MR elastography: repeatability and reproducibility analysis
title_full_unstemmed Evaluation of infrapatellar fat pad elasticity in knee osteoarthritis using IVIM-DWI-based virtual MR elastography: repeatability and reproducibility analysis
title_short Evaluation of infrapatellar fat pad elasticity in knee osteoarthritis using IVIM-DWI-based virtual MR elastography: repeatability and reproducibility analysis
title_sort evaluation of infrapatellar fat pad elasticity in knee osteoarthritis using ivim dwi based virtual mr elastography repeatability and reproducibility analysis
topic Intravoxel incoherent motion
Diffusion-weighted imaging
Elastography
Osteoarthritis
Infrapatellar fat pad
url https://doi.org/10.1186/s12891-025-08660-6
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