Genetic relationship among Trapa species assessed by RAPD markers
To assess the genetic relationship between 10 species of genus Trapa, 16 samples were used for RAPD assay. 18 primers were screened from the 50 random primer, and a total of 205 DNA bands were amplified, among which 165 (79.5%) were polymprphic. Based on the RAPD data, Nei's genetic identity an...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.02.0191 |
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| Summary: | To assess the genetic relationship between 10 species of genus Trapa, 16 samples were used for RAPD assay. 18 primers were screened from the 50 random primer, and a total of 205 DNA bands were amplified, among which 165 (79.5%) were polymprphic. Based on the RAPD data, Nei's genetic identity and genetic diatance among the samples were calculated and a dendrogram was constructed by using UPGMA's method. According to the RAPD analysis combined with field observation and the cluster analysis of morphological characters, we suggest that Trapa plants in China can be classified into 3 species, namely T. natans L., T. incisa Sieb. et Zucc. and T. maximowiczii Korsh. The usual four-horn characteristic of the fruits in T. natans and T. incisa, but not in T. maximowqiczii, can be changed under certain conditions so as to assume the shape of fruits with only two instead of four horns. Therefore, the reasonableness and reliability of taking the number of fruit horns as a criterion for discrimination between different species of Trapa are open to question. T. natans and T. japonicaare are most probably the wild or congener species from which the cultivated Trapa were evolved. The T. maximowiczii is believed to be the most original species of Trapa presently existing. |
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| ISSN: | 1008-9209 2097-5155 |