A Novel Linear Evaluation of Chromatographic Peak Features in Pharmacopoeias Using an Inverse Fourier Transform Algorithm
The system suitability testing of chromatography is an indispensable procedure in pharmaceutical analysis, and it must comply with rules in related pharmacopoeias. An inverse Fourier transform algorithm was developed to accurately evaluate chromatographic features versus a standard Gaussian peak sha...
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MDPI AG
2025-06-01
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| Series: | Biophysica |
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| Online Access: | https://www.mdpi.com/2673-4125/5/2/21 |
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| author | Shuping Chen Weiyuan Zhu Sai Huang Baoling Zheng |
| author_facet | Shuping Chen Weiyuan Zhu Sai Huang Baoling Zheng |
| author_sort | Shuping Chen |
| collection | DOAJ |
| description | The system suitability testing of chromatography is an indispensable procedure in pharmaceutical analysis, and it must comply with rules in related pharmacopoeias. An inverse Fourier transform algorithm was developed to accurately evaluate chromatographic features versus a standard Gaussian peak shape. The regular chromatogram is considered a pseudo-frequency spectrum and can be converted to a nominal time signal via inverse Fourier transformation. The system suitability parameters of peak width, theoretical plate number, tailing factor, and noise testing were evaluated using linear regressions directly and compared with the compendial rules. This novel method is simple, accurate, robust, reliable, and efficient for the evaluation of chromatographic peak features. |
| format | Article |
| id | doaj-art-b0dadcac1a624cf4baa949792dffd79b |
| institution | Kabale University |
| issn | 2673-4125 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biophysica |
| spelling | doaj-art-b0dadcac1a624cf4baa949792dffd79b2025-08-20T03:27:21ZengMDPI AGBiophysica2673-41252025-06-01522110.3390/biophysica5020021A Novel Linear Evaluation of Chromatographic Peak Features in Pharmacopoeias Using an Inverse Fourier Transform AlgorithmShuping Chen0Weiyuan Zhu1Sai Huang2Baoling Zheng3Nexus Scitech Centre of Canada, 17 White Oak Crescent, Richmond Hill, ON L4B 3R7, CanadaNexus Scitech Centre of Canada, 17 White Oak Crescent, Richmond Hill, ON L4B 3R7, CanadaFujian Superimposegraph Co., Ltd., Floor 20-1402. 338, Hualin Road, Fuzhou 350013, ChinaFujian Superimposegraph Co., Ltd., Floor 20-1402. 338, Hualin Road, Fuzhou 350013, ChinaThe system suitability testing of chromatography is an indispensable procedure in pharmaceutical analysis, and it must comply with rules in related pharmacopoeias. An inverse Fourier transform algorithm was developed to accurately evaluate chromatographic features versus a standard Gaussian peak shape. The regular chromatogram is considered a pseudo-frequency spectrum and can be converted to a nominal time signal via inverse Fourier transformation. The system suitability parameters of peak width, theoretical plate number, tailing factor, and noise testing were evaluated using linear regressions directly and compared with the compendial rules. This novel method is simple, accurate, robust, reliable, and efficient for the evaluation of chromatographic peak features.https://www.mdpi.com/2673-4125/5/2/21chromatographic peak featuresystem suitabilitypharmaceutical analysisinverse Fourier transformGaussian and distortion peaks |
| spellingShingle | Shuping Chen Weiyuan Zhu Sai Huang Baoling Zheng A Novel Linear Evaluation of Chromatographic Peak Features in Pharmacopoeias Using an Inverse Fourier Transform Algorithm Biophysica chromatographic peak feature system suitability pharmaceutical analysis inverse Fourier transform Gaussian and distortion peaks |
| title | A Novel Linear Evaluation of Chromatographic Peak Features in Pharmacopoeias Using an Inverse Fourier Transform Algorithm |
| title_full | A Novel Linear Evaluation of Chromatographic Peak Features in Pharmacopoeias Using an Inverse Fourier Transform Algorithm |
| title_fullStr | A Novel Linear Evaluation of Chromatographic Peak Features in Pharmacopoeias Using an Inverse Fourier Transform Algorithm |
| title_full_unstemmed | A Novel Linear Evaluation of Chromatographic Peak Features in Pharmacopoeias Using an Inverse Fourier Transform Algorithm |
| title_short | A Novel Linear Evaluation of Chromatographic Peak Features in Pharmacopoeias Using an Inverse Fourier Transform Algorithm |
| title_sort | novel linear evaluation of chromatographic peak features in pharmacopoeias using an inverse fourier transform algorithm |
| topic | chromatographic peak feature system suitability pharmaceutical analysis inverse Fourier transform Gaussian and distortion peaks |
| url | https://www.mdpi.com/2673-4125/5/2/21 |
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