Safety, probiotic potential, and mycotoxin-reduction efficacy of Limosilactobacillus fermentum isolated from traditional dairy products
Lactic acid bacteria (LAB) possess safety and preservative functions, particularly in dairy products. New LAB strains were isolated and defined from conventional dairy products for their probiotic potential and functional characteristics. The isolates were evaluated for safety (including hemolytic a...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-12-01
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| Series: | Applied Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772502225004081 |
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| Summary: | Lactic acid bacteria (LAB) possess safety and preservative functions, particularly in dairy products. New LAB strains were isolated and defined from conventional dairy products for their probiotic potential and functional characteristics. The isolates were evaluated for safety (including hemolytic activity and antibiotic sensitivity), tolerance to simulated gastric conditions, and adhesion to intestinal epithelial cells. Upon these results, two strains were selected for 16S rDNA identification and bacteriocin gene presence concerning their activities, namely Limosilactobacillus fermentum DMCR 309 and DMCR 316. The results indicate their probiotic potential and show marked anti-mycotic and anti-mycotoxigenic activities, low pathogenicity, high acid generation, and outstanding gastrointestinal tolerance. Cytotoxicity and antibacterial activity against pathogens and toxigenic fungi were assessed in cell-free supernatant solutions (CFSS). Strain DMCR 316 bound 39.5–44.4 % of aflatoxins and 77 % of OCA, and DMCR 309 bound 30–34 % of aflatoxins and 71 % of OCA. Especially strain DMCR 316 showed a total (100 %) OCA reduction and outstanding suppression of ochratoxin-producing fungus (87.08–88.3 %). Strong attachment to intestinal cells and minimal toxicity of both strains indicate their fit for functional foods and food safety uses. Due to their antimicrobial and mycotoxin-reduction activities, these strains have potential applications in dairy preservation, probiotic formulations, and biocontrol measures in food industries. Further, in vivo studies are required to validate their functional efficacy, safety, and colonization dynamics under physiological conditions. |
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| ISSN: | 2772-5022 |