Retinoic Acid-Related Orphan Receptor Alpha May Regulate the State of Hair Follicle Stem Cells by Upregulating the Expression of BNIP3

The hair, an exclusive keratinized dermal appendage in mammals, stands as a quintessential outcome of adaptive evolution, conferring resilience against adverse environmental conditions. The ontogenesis of the coat displays a pronounced rhythmic pattern, with hair follicle stem cells (HFSCs) emerging...

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Main Authors: Yu Zhang, Xuefei Zhao, Shuqi Li, Yanchun Xu, Suying Bai, Wei Zhang
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/14/23/3477
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author Yu Zhang
Xuefei Zhao
Shuqi Li
Yanchun Xu
Suying Bai
Wei Zhang
author_facet Yu Zhang
Xuefei Zhao
Shuqi Li
Yanchun Xu
Suying Bai
Wei Zhang
author_sort Yu Zhang
collection DOAJ
description The hair, an exclusive keratinized dermal appendage in mammals, stands as a quintessential outcome of adaptive evolution, conferring resilience against adverse environmental conditions. The ontogenesis of the coat displays a pronounced rhythmic pattern, with hair follicle stem cells (HFSCs) emerging as pivotal facilitators of hair follicle reconstitution. The retinoic acid-related orphan receptor alpha, a nuclear receptor with extensive involvement in the regulation of cellular physiological states, exerts its functions predominantly through the modulation of downstream target gene transcription. The <i>Bnip3</i> gene exhibits a robust correlation with cellular apoptosis and autophagy, which are indispensable physiological mechanisms underlying the maintenance of HFSC homeostasis. Consequently, the expression level of <i>Bnip3</i> may be intimately linked to the status of HFSCs. In this investigative endeavor, we employed rat HFSCs as a model system to validate the regulatory impact of RORA on <i>Bnip3</i> gene expression. Our findings unequivocally demonstrate that <i>Bnip3</i> serves as a direct downstream target of RORA. Specifically, RORA binds to the motif within the <i>Bnip3</i> promoter region, thereby upregulating <i>Bnip3</i> expression levels. In light of our research findings, we propose that RORA holds potential as a target for modulating the status of HFSCs.
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spelling doaj-art-b0d15516f1ef449ea4e5c95d4937db9d2025-08-20T01:55:34ZengMDPI AGAnimals2076-26152024-12-011423347710.3390/ani14233477Retinoic Acid-Related Orphan Receptor Alpha May Regulate the State of Hair Follicle Stem Cells by Upregulating the Expression of BNIP3Yu Zhang0Xuefei Zhao1Shuqi Li2Yanchun Xu3Suying Bai4Wei Zhang5College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, ChinaCollege of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, ChinaCollege of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, ChinaCollege of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, ChinaCollege of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, ChinaCollege of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, ChinaThe hair, an exclusive keratinized dermal appendage in mammals, stands as a quintessential outcome of adaptive evolution, conferring resilience against adverse environmental conditions. The ontogenesis of the coat displays a pronounced rhythmic pattern, with hair follicle stem cells (HFSCs) emerging as pivotal facilitators of hair follicle reconstitution. The retinoic acid-related orphan receptor alpha, a nuclear receptor with extensive involvement in the regulation of cellular physiological states, exerts its functions predominantly through the modulation of downstream target gene transcription. The <i>Bnip3</i> gene exhibits a robust correlation with cellular apoptosis and autophagy, which are indispensable physiological mechanisms underlying the maintenance of HFSC homeostasis. Consequently, the expression level of <i>Bnip3</i> may be intimately linked to the status of HFSCs. In this investigative endeavor, we employed rat HFSCs as a model system to validate the regulatory impact of RORA on <i>Bnip3</i> gene expression. Our findings unequivocally demonstrate that <i>Bnip3</i> serves as a direct downstream target of RORA. Specifically, RORA binds to the motif within the <i>Bnip3</i> promoter region, thereby upregulating <i>Bnip3</i> expression levels. In light of our research findings, we propose that RORA holds potential as a target for modulating the status of HFSCs.https://www.mdpi.com/2076-2615/14/23/3477hair follicle stem cellsretinoic acid-related orphan receptor alpha<i>Bnip3</i>
spellingShingle Yu Zhang
Xuefei Zhao
Shuqi Li
Yanchun Xu
Suying Bai
Wei Zhang
Retinoic Acid-Related Orphan Receptor Alpha May Regulate the State of Hair Follicle Stem Cells by Upregulating the Expression of BNIP3
Animals
hair follicle stem cells
retinoic acid-related orphan receptor alpha
<i>Bnip3</i>
title Retinoic Acid-Related Orphan Receptor Alpha May Regulate the State of Hair Follicle Stem Cells by Upregulating the Expression of BNIP3
title_full Retinoic Acid-Related Orphan Receptor Alpha May Regulate the State of Hair Follicle Stem Cells by Upregulating the Expression of BNIP3
title_fullStr Retinoic Acid-Related Orphan Receptor Alpha May Regulate the State of Hair Follicle Stem Cells by Upregulating the Expression of BNIP3
title_full_unstemmed Retinoic Acid-Related Orphan Receptor Alpha May Regulate the State of Hair Follicle Stem Cells by Upregulating the Expression of BNIP3
title_short Retinoic Acid-Related Orphan Receptor Alpha May Regulate the State of Hair Follicle Stem Cells by Upregulating the Expression of BNIP3
title_sort retinoic acid related orphan receptor alpha may regulate the state of hair follicle stem cells by upregulating the expression of bnip3
topic hair follicle stem cells
retinoic acid-related orphan receptor alpha
<i>Bnip3</i>
url https://www.mdpi.com/2076-2615/14/23/3477
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