Antioxidant, antidiabetic and molecular docking studies of D-pinitol isolated from Cleistopholis patens (Benth) leaf extract
Abstract Cleistopholis patens is a medicinal plant with secondary metabolites that possess diverse biological activities that are beneficial to humans. It is used in the management of diabetes as well as treatment of jaundice, infective hepatitis, stomach disorders among others. Synthetic drugs used...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2025-05-01
|
| Series: | Discover Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s44371-025-00200-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850243537034018816 |
|---|---|
| author | Olukayode Solomon Ajayi Emmanuel Gabriel Fakola Fiyinfoluwa Funmibi Olubiyo Olaoye Solomon Balogun Idowu Julius Olawuni Tawakalit Omolara Kelani |
| author_facet | Olukayode Solomon Ajayi Emmanuel Gabriel Fakola Fiyinfoluwa Funmibi Olubiyo Olaoye Solomon Balogun Idowu Julius Olawuni Tawakalit Omolara Kelani |
| author_sort | Olukayode Solomon Ajayi |
| collection | DOAJ |
| description | Abstract Cleistopholis patens is a medicinal plant with secondary metabolites that possess diverse biological activities that are beneficial to humans. It is used in the management of diabetes as well as treatment of jaundice, infective hepatitis, stomach disorders among others. Synthetic drugs used for treatment of diabetes mellitus often have side effects, hence, the search for natural sources of antidiabetic drug. This study set out to evaluate the phytochemicals, antioxidant capacity effects of the solvent fractions and enzyme inhibition of the isolated compound from Cleistopholis patens (Benth) leaf. The extracts were further evaluated for the total phenolic (TPC) and flavonoid content (TFC). The aqueous methanol fraction was subjected to column chromatography over stationary phases such as silica gel and sephadex LH-20, the isolated compound 1; (D-pinitol) were characterized using IR and NMR spectroscopy techniques. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, Ferric reducing antioxidant power (FRAP) and Total antioxidant capacity (TAC) assays were used to evaluate antioxidant potentials of the extract and solvent fractions. Furthermore, in silico molecular docking and ADMET analysis were carried out on the only isolated compound (D-pinitol) with other standard antidiabetic drugs to predict the its pharmacokinetic indicators and toxicity. The results exhibited that the ethyl acetate fraction has higher levels of total phenolics (129.16 ± 5.59 mg GAE/g) and flavonoids (209.84 ± 2.82 mg QUE/g) compared with the crude methanol extract (73.61 ± 2.43 mg GAE/g and 76.99 ± 9.99 mg QUE/g) respectively. The antioxidant activity studies revealed that ethyl acetate fraction demonstrated higher radical scavenging in DPPH (IC50 of 0.08 ± 0.02 mg/mL) and TAC (222.28 ± 8.71 mg AAE/g) respectively. While for reducing abilities (FRAP), ethyl acetate fraction has also the highest activity (50.22 ± 3.28 mg AAE/g) than the crude methanol extract (30.61 ± 1.74 mg AAE/g). D-pinitol exhibited α-amylase and α-glucosidase inhibitory activity with IC50 values of 59.61 ± 0.71 µg/mL and 124.83 ± 0.06 µg/mL respectively, compared with acarbose (standard drug) with 16.32 ± 0.01 µg/mL and 73.67 ± 0.02 µg/mL. Therefore, this study investigates the antioxidant potential, in vitro α-amylase and α-glucosidase inhibitory activities of Cleistopholis patens leaf extract and isolated compound, D-Pinitol. |
| format | Article |
| id | doaj-art-b0ce23feb92148a38546a0c2239fe9a8 |
| institution | OA Journals |
| issn | 3005-1193 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Chemistry |
| spelling | doaj-art-b0ce23feb92148a38546a0c2239fe9a82025-08-20T01:59:57ZengSpringerDiscover Chemistry3005-11932025-05-012111410.1007/s44371-025-00200-4Antioxidant, antidiabetic and molecular docking studies of D-pinitol isolated from Cleistopholis patens (Benth) leaf extractOlukayode Solomon Ajayi0Emmanuel Gabriel Fakola1Fiyinfoluwa Funmibi Olubiyo2Olaoye Solomon Balogun3Idowu Julius Olawuni4Tawakalit Omolara Kelani5Department of Chemistry, Obafemi Awolowo UniversityDepartment of Chemistry, Obafemi Awolowo UniversityDepartment of Chemistry, Obafemi Awolowo UniversityDepartment of Chemistry, Obafemi Awolowo UniversityDepartment of Biochemistry and Molecular Biology, Obafemi Awolowo UniversityDepartment of Industrial Chemistry, Edo University IyamhoAbstract Cleistopholis patens is a medicinal plant with secondary metabolites that possess diverse biological activities that are beneficial to humans. It is used in the management of diabetes as well as treatment of jaundice, infective hepatitis, stomach disorders among others. Synthetic drugs used for treatment of diabetes mellitus often have side effects, hence, the search for natural sources of antidiabetic drug. This study set out to evaluate the phytochemicals, antioxidant capacity effects of the solvent fractions and enzyme inhibition of the isolated compound from Cleistopholis patens (Benth) leaf. The extracts were further evaluated for the total phenolic (TPC) and flavonoid content (TFC). The aqueous methanol fraction was subjected to column chromatography over stationary phases such as silica gel and sephadex LH-20, the isolated compound 1; (D-pinitol) were characterized using IR and NMR spectroscopy techniques. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, Ferric reducing antioxidant power (FRAP) and Total antioxidant capacity (TAC) assays were used to evaluate antioxidant potentials of the extract and solvent fractions. Furthermore, in silico molecular docking and ADMET analysis were carried out on the only isolated compound (D-pinitol) with other standard antidiabetic drugs to predict the its pharmacokinetic indicators and toxicity. The results exhibited that the ethyl acetate fraction has higher levels of total phenolics (129.16 ± 5.59 mg GAE/g) and flavonoids (209.84 ± 2.82 mg QUE/g) compared with the crude methanol extract (73.61 ± 2.43 mg GAE/g and 76.99 ± 9.99 mg QUE/g) respectively. The antioxidant activity studies revealed that ethyl acetate fraction demonstrated higher radical scavenging in DPPH (IC50 of 0.08 ± 0.02 mg/mL) and TAC (222.28 ± 8.71 mg AAE/g) respectively. While for reducing abilities (FRAP), ethyl acetate fraction has also the highest activity (50.22 ± 3.28 mg AAE/g) than the crude methanol extract (30.61 ± 1.74 mg AAE/g). D-pinitol exhibited α-amylase and α-glucosidase inhibitory activity with IC50 values of 59.61 ± 0.71 µg/mL and 124.83 ± 0.06 µg/mL respectively, compared with acarbose (standard drug) with 16.32 ± 0.01 µg/mL and 73.67 ± 0.02 µg/mL. Therefore, this study investigates the antioxidant potential, in vitro α-amylase and α-glucosidase inhibitory activities of Cleistopholis patens leaf extract and isolated compound, D-Pinitol.https://doi.org/10.1007/s44371-025-00200-4Cleistopholis patensAntioxidantα-amylase, α-glucosidaseMolecular docking |
| spellingShingle | Olukayode Solomon Ajayi Emmanuel Gabriel Fakola Fiyinfoluwa Funmibi Olubiyo Olaoye Solomon Balogun Idowu Julius Olawuni Tawakalit Omolara Kelani Antioxidant, antidiabetic and molecular docking studies of D-pinitol isolated from Cleistopholis patens (Benth) leaf extract Discover Chemistry Cleistopholis patens Antioxidant α-amylase, α-glucosidase Molecular docking |
| title | Antioxidant, antidiabetic and molecular docking studies of D-pinitol isolated from Cleistopholis patens (Benth) leaf extract |
| title_full | Antioxidant, antidiabetic and molecular docking studies of D-pinitol isolated from Cleistopholis patens (Benth) leaf extract |
| title_fullStr | Antioxidant, antidiabetic and molecular docking studies of D-pinitol isolated from Cleistopholis patens (Benth) leaf extract |
| title_full_unstemmed | Antioxidant, antidiabetic and molecular docking studies of D-pinitol isolated from Cleistopholis patens (Benth) leaf extract |
| title_short | Antioxidant, antidiabetic and molecular docking studies of D-pinitol isolated from Cleistopholis patens (Benth) leaf extract |
| title_sort | antioxidant antidiabetic and molecular docking studies of d pinitol isolated from cleistopholis patens benth leaf extract |
| topic | Cleistopholis patens Antioxidant α-amylase, α-glucosidase Molecular docking |
| url | https://doi.org/10.1007/s44371-025-00200-4 |
| work_keys_str_mv | AT olukayodesolomonajayi antioxidantantidiabeticandmoleculardockingstudiesofdpinitolisolatedfromcleistopholispatensbenthleafextract AT emmanuelgabrielfakola antioxidantantidiabeticandmoleculardockingstudiesofdpinitolisolatedfromcleistopholispatensbenthleafextract AT fiyinfoluwafunmibiolubiyo antioxidantantidiabeticandmoleculardockingstudiesofdpinitolisolatedfromcleistopholispatensbenthleafextract AT olaoyesolomonbalogun antioxidantantidiabeticandmoleculardockingstudiesofdpinitolisolatedfromcleistopholispatensbenthleafextract AT idowujuliusolawuni antioxidantantidiabeticandmoleculardockingstudiesofdpinitolisolatedfromcleistopholispatensbenthleafextract AT tawakalitomolarakelani antioxidantantidiabeticandmoleculardockingstudiesofdpinitolisolatedfromcleistopholispatensbenthleafextract |