Comparative Metabolite Profiling of Antarctic and Korean Mosses: Insights into Adaptation Mechanisms of Antarctic Moss Species

This study investigates the relationship between secondary metabolites and stress tolerance in moss species, with a specific emphasis on comparing Antarctic and Korean mosses. Analyses of total phenolic content (TPC) and total flavonoid content (TFC) revealed that Antarctic mosses contain these comp...

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Main Authors: Marufa Naznin, Raisul Awal Mahmood, Md Badrul Alam, Kil Ho Shin, Kyungwon Min, Sang-Han Lee, Hyoungseok Lee, Sunghwan Kim
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/14/2148
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author Marufa Naznin
Raisul Awal Mahmood
Md Badrul Alam
Kil Ho Shin
Kyungwon Min
Sang-Han Lee
Hyoungseok Lee
Sunghwan Kim
author_facet Marufa Naznin
Raisul Awal Mahmood
Md Badrul Alam
Kil Ho Shin
Kyungwon Min
Sang-Han Lee
Hyoungseok Lee
Sunghwan Kim
author_sort Marufa Naznin
collection DOAJ
description This study investigates the relationship between secondary metabolites and stress tolerance in moss species, with a specific emphasis on comparing Antarctic and Korean mosses. Analyses of total phenolic content (TPC) and total flavonoid content (TFC) revealed that Antarctic mosses contain these compounds at significantly higher levels compared to the Korean mosses. These findings are consistent with greater antioxidant activities observed in Antarctic mosses through DPPH and ABTS<sup>•+</sup> radical scavenging assays. In this study, a total of 620 metabolites were identified from the moss samples. The results showed that Antarctic mosses exhibited a high number and diversity of compounds including terpenoids, flavonoids, lipids, and other classes. Additionally, Antarctic mosses had fewer lipids with carbon chain lengths below 18 and a higher content of unsaturated lipids, indicating adaptations to maintain membrane fluidity under cold stress. The phylogenetic relationships suggested a correlation between metabolite profiles and genetic adaptations between these species. This research highlights the complex biochemical strategies that mosses, particularly those in Antarctic regions, employ to adapt the environmental stressors. The high abundance of secondary metabolites in Antarctic mosses not only serves as a defense mechanism against oxidative stress but also suggests their potential applications in various biotechnological aspects. This study reveals new avenues for exploring the ecological roles and potential uses of these resilient plant species.
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spelling doaj-art-b0ca1f6a87d8429fab2ecf42736d32402025-08-20T03:56:46ZengMDPI AGPlants2223-77472025-07-011414214810.3390/plants14142148Comparative Metabolite Profiling of Antarctic and Korean Mosses: Insights into Adaptation Mechanisms of Antarctic Moss SpeciesMarufa Naznin0Raisul Awal Mahmood1Md Badrul Alam2Kil Ho Shin3Kyungwon Min4Sang-Han Lee5Hyoungseok Lee6Sunghwan Kim7Department of Chemistry, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Chemistry, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Republic of KoreaParkor Bio & Green, Incheon 21532, Republic of KoreaDivision of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of KoreaDepartment of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Republic of KoreaDivision of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of KoreaDepartment of Chemistry, Kyungpook National University, Daegu 41566, Republic of KoreaThis study investigates the relationship between secondary metabolites and stress tolerance in moss species, with a specific emphasis on comparing Antarctic and Korean mosses. Analyses of total phenolic content (TPC) and total flavonoid content (TFC) revealed that Antarctic mosses contain these compounds at significantly higher levels compared to the Korean mosses. These findings are consistent with greater antioxidant activities observed in Antarctic mosses through DPPH and ABTS<sup>•+</sup> radical scavenging assays. In this study, a total of 620 metabolites were identified from the moss samples. The results showed that Antarctic mosses exhibited a high number and diversity of compounds including terpenoids, flavonoids, lipids, and other classes. Additionally, Antarctic mosses had fewer lipids with carbon chain lengths below 18 and a higher content of unsaturated lipids, indicating adaptations to maintain membrane fluidity under cold stress. The phylogenetic relationships suggested a correlation between metabolite profiles and genetic adaptations between these species. This research highlights the complex biochemical strategies that mosses, particularly those in Antarctic regions, employ to adapt the environmental stressors. The high abundance of secondary metabolites in Antarctic mosses not only serves as a defense mechanism against oxidative stress but also suggests their potential applications in various biotechnological aspects. This study reveals new avenues for exploring the ecological roles and potential uses of these resilient plant species.https://www.mdpi.com/2223-7747/14/14/2148Antarctic mossantioxidantstress responsechanges in metabolite profile
spellingShingle Marufa Naznin
Raisul Awal Mahmood
Md Badrul Alam
Kil Ho Shin
Kyungwon Min
Sang-Han Lee
Hyoungseok Lee
Sunghwan Kim
Comparative Metabolite Profiling of Antarctic and Korean Mosses: Insights into Adaptation Mechanisms of Antarctic Moss Species
Plants
Antarctic moss
antioxidant
stress response
changes in metabolite profile
title Comparative Metabolite Profiling of Antarctic and Korean Mosses: Insights into Adaptation Mechanisms of Antarctic Moss Species
title_full Comparative Metabolite Profiling of Antarctic and Korean Mosses: Insights into Adaptation Mechanisms of Antarctic Moss Species
title_fullStr Comparative Metabolite Profiling of Antarctic and Korean Mosses: Insights into Adaptation Mechanisms of Antarctic Moss Species
title_full_unstemmed Comparative Metabolite Profiling of Antarctic and Korean Mosses: Insights into Adaptation Mechanisms of Antarctic Moss Species
title_short Comparative Metabolite Profiling of Antarctic and Korean Mosses: Insights into Adaptation Mechanisms of Antarctic Moss Species
title_sort comparative metabolite profiling of antarctic and korean mosses insights into adaptation mechanisms of antarctic moss species
topic Antarctic moss
antioxidant
stress response
changes in metabolite profile
url https://www.mdpi.com/2223-7747/14/14/2148
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