Integrated two-photon and optoacoustic microscopy for functional neuroimaging
Abstract Understanding the interaction between cerebral vasculature and neurons is critical for studying neurovascular processes and their roles in brain function and neurological disorders. Existing functional neuroimaging approaches face trade-offs between resolution, penetration depth, and spatio...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-07819-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849238644114587648 |
|---|---|
| author | Shruti Sundar Tian Jin Yu-Hang Liu Zhenyue Chen Michael Reiss Alexey Kurnikov Pavel Subochev Daniel Razansky |
| author_facet | Shruti Sundar Tian Jin Yu-Hang Liu Zhenyue Chen Michael Reiss Alexey Kurnikov Pavel Subochev Daniel Razansky |
| author_sort | Shruti Sundar |
| collection | DOAJ |
| description | Abstract Understanding the interaction between cerebral vasculature and neurons is critical for studying neurovascular processes and their roles in brain function and neurological disorders. Existing functional neuroimaging approaches face trade-offs between resolution, penetration depth, and spatiotemporal alignment, limiting their ability to comprehensively image neurovascular anatomy and function in vivo. To address this challenge, we developed a dual-modality system that combines optical-resolution optoacoustic microscopy with two-photon fluorescence microscopy. The system enables imaging of microcapillaries with submicron resolution at up to 140 μm depth and neurons beyond 300 μm depth in the mouse cortex, thus providing complementary information. Using a semi-simultaneous acquisition protocol, the system alternately captures data across time and depth planes, ensuring spatiotemporal alignment, minimizing motion artifacts, and enabling robust co-registration of multimodal datasets for comprehensive studies of neurovascular coupling in health and disease. |
| format | Article |
| id | doaj-art-b0c884d5ed674d6ea7ebd3db7fd1a966 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-b0c884d5ed674d6ea7ebd3db7fd1a9662025-08-20T04:01:26ZengNature PortfolioScientific Reports2045-23222025-07-0115111010.1038/s41598-025-07819-4Integrated two-photon and optoacoustic microscopy for functional neuroimagingShruti Sundar0Tian Jin1Yu-Hang Liu2Zhenyue Chen3Michael Reiss4Alexey Kurnikov5Pavel Subochev6Daniel Razansky7Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering , Faculty of Medicine, University of ZurichInstitute of Pharmacology and Toxicology and Institute for Biomedical Engineering , Faculty of Medicine, University of ZurichInstitute of Pharmacology and Toxicology and Institute for Biomedical Engineering , Faculty of Medicine, University of ZurichSchool of Physics Science and Engineering, Tongji UniversityInstitute of Pharmacology and Toxicology and Institute for Biomedical Engineering , Faculty of Medicine, University of ZurichInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical UniversityInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical UniversityInstitute of Pharmacology and Toxicology and Institute for Biomedical Engineering , Faculty of Medicine, University of ZurichAbstract Understanding the interaction between cerebral vasculature and neurons is critical for studying neurovascular processes and their roles in brain function and neurological disorders. Existing functional neuroimaging approaches face trade-offs between resolution, penetration depth, and spatiotemporal alignment, limiting their ability to comprehensively image neurovascular anatomy and function in vivo. To address this challenge, we developed a dual-modality system that combines optical-resolution optoacoustic microscopy with two-photon fluorescence microscopy. The system enables imaging of microcapillaries with submicron resolution at up to 140 μm depth and neurons beyond 300 μm depth in the mouse cortex, thus providing complementary information. Using a semi-simultaneous acquisition protocol, the system alternately captures data across time and depth planes, ensuring spatiotemporal alignment, minimizing motion artifacts, and enabling robust co-registration of multimodal datasets for comprehensive studies of neurovascular coupling in health and disease.https://doi.org/10.1038/s41598-025-07819-4Dual-modality imagingTwo-photon fluorescence microscopyOptoacoustic microscopyFunctional neuroimaging |
| spellingShingle | Shruti Sundar Tian Jin Yu-Hang Liu Zhenyue Chen Michael Reiss Alexey Kurnikov Pavel Subochev Daniel Razansky Integrated two-photon and optoacoustic microscopy for functional neuroimaging Scientific Reports Dual-modality imaging Two-photon fluorescence microscopy Optoacoustic microscopy Functional neuroimaging |
| title | Integrated two-photon and optoacoustic microscopy for functional neuroimaging |
| title_full | Integrated two-photon and optoacoustic microscopy for functional neuroimaging |
| title_fullStr | Integrated two-photon and optoacoustic microscopy for functional neuroimaging |
| title_full_unstemmed | Integrated two-photon and optoacoustic microscopy for functional neuroimaging |
| title_short | Integrated two-photon and optoacoustic microscopy for functional neuroimaging |
| title_sort | integrated two photon and optoacoustic microscopy for functional neuroimaging |
| topic | Dual-modality imaging Two-photon fluorescence microscopy Optoacoustic microscopy Functional neuroimaging |
| url | https://doi.org/10.1038/s41598-025-07819-4 |
| work_keys_str_mv | AT shrutisundar integratedtwophotonandoptoacousticmicroscopyforfunctionalneuroimaging AT tianjin integratedtwophotonandoptoacousticmicroscopyforfunctionalneuroimaging AT yuhangliu integratedtwophotonandoptoacousticmicroscopyforfunctionalneuroimaging AT zhenyuechen integratedtwophotonandoptoacousticmicroscopyforfunctionalneuroimaging AT michaelreiss integratedtwophotonandoptoacousticmicroscopyforfunctionalneuroimaging AT alexeykurnikov integratedtwophotonandoptoacousticmicroscopyforfunctionalneuroimaging AT pavelsubochev integratedtwophotonandoptoacousticmicroscopyforfunctionalneuroimaging AT danielrazansky integratedtwophotonandoptoacousticmicroscopyforfunctionalneuroimaging |