In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D
<p class="IsiAbstrakIndo"><span lang="EN-GB">The addition of growth regulator is one of the critical success factors in in vitro cultures. 2,4-D as a plant regulator in media can stimulate the cell division, enlargement of the explants and promotes the formation and g...
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Universitas Negeri Semarang
2017-12-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/11018 |
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| author | Iman Budisantoso Nurul Amalia Kamsinah Kamsinah |
| author_facet | Iman Budisantoso Nurul Amalia Kamsinah Kamsinah |
| author_sort | Iman Budisantoso |
| collection | DOAJ |
| description | <p class="IsiAbstrakIndo"><span lang="EN-GB">The addition of growth regulator is one of the critical success factors in in vitro cultures. 2,4-D as a plant regulator in media can stimulate the cell division, enlargement of the explants and promotes the formation and growth of callus. The purpose of this study was to determine the time of callus formation and to determine the best concentration of 2.4-D in inducing the growth of callus from leaf explants of </span><em><span lang="EN-GB">Vanda</span></em><span lang="EN-GB"> sp. This research was conducted by experiment with completely randomized design, which consists of six levels of treatment concentration of 2,4-D i.e. 0 ppm; 1 ppm; 1.5 ppm; 2 ppm; 2.5 ppm; and 3 ppm. The parameters observed were the percentage of callus formation and the form of callus from </span><em><span lang="EN-GB">Vanda</span></em><span lang="EN-GB"> sp leaf explants. The results were statistically analyzed by using MINITAB program version 17. Analysis of variance (ANOVA) was performed and the difference between means score/value was separated by F test at p < 0.05. The results showed that 2,4-D treatment give significant effect (F 5,12 = 3,20; p = 0,046 < 0,05) on the callus growth time and its percentage. Application of 2 ppm 2.4-D was the best concentration for accelerating the callus growth time (14.3 days after planting) and increasing the percentage of callus formation (83.3%). Most of callus type were proliferative callus (36.11%) and senescence callus (11.11%). The results of this research are very important to grow the callus from Vanda leaves orchid explant because it is very diffucult to grow.</span></p> |
| format | Article |
| id | doaj-art-b0c720c0bbf54b9aa7abe69f48a6fa24 |
| institution | DOAJ |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-b0c720c0bbf54b9aa7abe69f48a6fa242025-08-20T03:04:34ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102017-12-019349249710.15294/biosaintifika.v9i3.110186586In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-DIman Budisantoso0Nurul Amalia1Kamsinah Kamsinah2Faculty of Biology, Universitas Jenderal Soedirman, IndonesiaFaculty of Biology, Universitas Jenderal SoedirmanFaculty of Biology, Universitas Jenderal Soedirman, Indonesia<p class="IsiAbstrakIndo"><span lang="EN-GB">The addition of growth regulator is one of the critical success factors in in vitro cultures. 2,4-D as a plant regulator in media can stimulate the cell division, enlargement of the explants and promotes the formation and growth of callus. The purpose of this study was to determine the time of callus formation and to determine the best concentration of 2.4-D in inducing the growth of callus from leaf explants of </span><em><span lang="EN-GB">Vanda</span></em><span lang="EN-GB"> sp. This research was conducted by experiment with completely randomized design, which consists of six levels of treatment concentration of 2,4-D i.e. 0 ppm; 1 ppm; 1.5 ppm; 2 ppm; 2.5 ppm; and 3 ppm. The parameters observed were the percentage of callus formation and the form of callus from </span><em><span lang="EN-GB">Vanda</span></em><span lang="EN-GB"> sp leaf explants. The results were statistically analyzed by using MINITAB program version 17. Analysis of variance (ANOVA) was performed and the difference between means score/value was separated by F test at p < 0.05. The results showed that 2,4-D treatment give significant effect (F 5,12 = 3,20; p = 0,046 < 0,05) on the callus growth time and its percentage. Application of 2 ppm 2.4-D was the best concentration for accelerating the callus growth time (14.3 days after planting) and increasing the percentage of callus formation (83.3%). Most of callus type were proliferative callus (36.11%) and senescence callus (11.11%). The results of this research are very important to grow the callus from Vanda leaves orchid explant because it is very diffucult to grow.</span></p>https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/11018Callus InductionLeaf ExplantVanda, 2,4-D |
| spellingShingle | Iman Budisantoso Nurul Amalia Kamsinah Kamsinah In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D Biosaintifika: Journal of Biology & Biology Education Callus Induction Leaf Explant Vanda, 2,4-D |
| title | In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D |
| title_full | In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D |
| title_fullStr | In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D |
| title_full_unstemmed | In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D |
| title_short | In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D |
| title_sort | in vitro callus induction from leaf explants of vanda sp stimulated by 2 4 d |
| topic | Callus Induction Leaf Explant Vanda, 2,4-D |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/11018 |
| work_keys_str_mv | AT imanbudisantoso invitrocallusinductionfromleafexplantsofvandaspstimulatedby24d AT nurulamalia invitrocallusinductionfromleafexplantsofvandaspstimulatedby24d AT kamsinahkamsinah invitrocallusinductionfromleafexplantsofvandaspstimulatedby24d |