Macromolecular crystallography and biology at the Linac Coherent Light Source

The Linac Coherent Light Source (LCLS) has significantly impacted the field of biology by providing advanced capabilities for probing the structure and dynamics of biological molecules with high precision. The ultrashort coherent X-ray pulses from the LCLS have enabled ultrafast, time-resolved, seri...

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Main Authors: Sandra Mous, Mark S. Hunter, Frédéric Poitevin, Sébastien Boutet, Leland B. Gee
Format: Article
Language:English
Published: International Union of Crystallography 2025-05-01
Series:Journal of Synchrotron Radiation
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Online Access:https://journals.iucr.org/paper?S1600577525002735
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author Sandra Mous
Mark S. Hunter
Frédéric Poitevin
Sébastien Boutet
Leland B. Gee
author_facet Sandra Mous
Mark S. Hunter
Frédéric Poitevin
Sébastien Boutet
Leland B. Gee
author_sort Sandra Mous
collection DOAJ
description The Linac Coherent Light Source (LCLS) has significantly impacted the field of biology by providing advanced capabilities for probing the structure and dynamics of biological molecules with high precision. The ultrashort coherent X-ray pulses from the LCLS have enabled ultrafast, time-resolved, serial femtosecond crystallography that is inaccessible at conventional synchrotron light sources. Since the facility's founding, scientists have captured detailed insights into biological processes at atomic resolution and fundamental timescales. The ability to observe these processes in real time and under conditions closely resembling their natural state is transforming our approach to studying biochemical mechanisms and developing new medical and energy applications. This work recounts some of the history of the LCLS, advances in biological research enabled by the LCLS, key biological areas that have been impacted and how the LCLS has helped to unravel complex biological phenomena in these fields.
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spelling doaj-art-b0ba54ef294d49068f6fa1f44f476da32025-08-20T02:28:27ZengInternational Union of CrystallographyJournal of Synchrotron Radiation1600-57752025-05-0132354856610.1107/S1600577525002735he5688Macromolecular crystallography and biology at the Linac Coherent Light SourceSandra Mous0Mark S. Hunter1Frédéric Poitevin2Sébastien Boutet3Leland B. Gee4Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USALinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USALinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USALinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USALinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USAThe Linac Coherent Light Source (LCLS) has significantly impacted the field of biology by providing advanced capabilities for probing the structure and dynamics of biological molecules with high precision. The ultrashort coherent X-ray pulses from the LCLS have enabled ultrafast, time-resolved, serial femtosecond crystallography that is inaccessible at conventional synchrotron light sources. Since the facility's founding, scientists have captured detailed insights into biological processes at atomic resolution and fundamental timescales. The ability to observe these processes in real time and under conditions closely resembling their natural state is transforming our approach to studying biochemical mechanisms and developing new medical and energy applications. This work recounts some of the history of the LCLS, advances in biological research enabled by the LCLS, key biological areas that have been impacted and how the LCLS has helped to unravel complex biological phenomena in these fields.https://journals.iucr.org/paper?S1600577525002735x-ray free-electron lasersserial femtosecond crystallographyprotein crystallographytime-resolved crystallographyroom-temperature crystallography
spellingShingle Sandra Mous
Mark S. Hunter
Frédéric Poitevin
Sébastien Boutet
Leland B. Gee
Macromolecular crystallography and biology at the Linac Coherent Light Source
Journal of Synchrotron Radiation
x-ray free-electron lasers
serial femtosecond crystallography
protein crystallography
time-resolved crystallography
room-temperature crystallography
title Macromolecular crystallography and biology at the Linac Coherent Light Source
title_full Macromolecular crystallography and biology at the Linac Coherent Light Source
title_fullStr Macromolecular crystallography and biology at the Linac Coherent Light Source
title_full_unstemmed Macromolecular crystallography and biology at the Linac Coherent Light Source
title_short Macromolecular crystallography and biology at the Linac Coherent Light Source
title_sort macromolecular crystallography and biology at the linac coherent light source
topic x-ray free-electron lasers
serial femtosecond crystallography
protein crystallography
time-resolved crystallography
room-temperature crystallography
url https://journals.iucr.org/paper?S1600577525002735
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AT markshunter macromolecularcrystallographyandbiologyatthelinaccoherentlightsource
AT fredericpoitevin macromolecularcrystallographyandbiologyatthelinaccoherentlightsource
AT sebastienboutet macromolecularcrystallographyandbiologyatthelinaccoherentlightsource
AT lelandbgee macromolecularcrystallographyandbiologyatthelinaccoherentlightsource